TY  - JOUR
AU  - Dorm, Bruna Carolina
AU  - Iemma, Mônica Rosas Costa
AU  - Neto, Benedito Domingos
AU  - Francisco, Rauany Cristina Lopes
AU  - Dinić, Ivana
AU  - Ignjatović, Nenad
AU  - Marković, Smilja
AU  - Vuković, Marina
AU  - Škapin, Srečo
AU  - Trovatti, Eliane
AU  - Mančić, Lidija
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/13685
AB  - Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8–25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine.
T2  - Life
T1  - Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles
SP  - 116
VL  - 13
IS  - 1
DO  - 10.3390/life13010116
UR  - https://hdl.handle.net/21.15107/rcub_dais_13685
ER  - 

@article{
author = "Dorm, Bruna Carolina and Iemma, Mônica Rosas Costa and Neto, Benedito Domingos and Francisco, Rauany Cristina Lopes and Dinić, Ivana and Ignjatović, Nenad and Marković, Smilja and Vuković, Marina and Škapin, Srečo and Trovatti, Eliane and Mančić, Lidija",
year = "2023",
abstract = "Hydroxyapatite attracts great attention as hard tissues implant material for bones and teeth. Its application in reconstructive medicine depends on its biocompatibility, which is in a function of composition and surface properties. The insertion of a protein element in the composition of implants can improve the cell adhesion and the osseointegration. Having this in mind, the proposal of this work was to develop L-alanine-grafted hydroxyapatite nanoparticles and to study their biocompatibility. Two L-alanine sources and three grafting methods were used for hydroxyapatite surface functionalization. The efficiency of grafting was determined based on X-ray powder diffraction, Fourier-transform infrared spectroscopy, thermal analyses, and field-emission scanning electron microscopy. The results indicated the formation of hydroxyapatite with 8–25 wt% of organic content, depending on the grafting method. Protein adsorption, cell adhesion, and viability studies were carried out to evaluate biological properties of grafted materials. The viability of MG-63 human osteoblastic cells following 24 h incubation with the alanine-grafted hydroxyapatite samples is well preserved, being in all cases above the viability of cells incubated with hydroxyapatite. The alanine-grafted hydroxyapatite prepared in situ and by simple mixture showed higher protein adsorption and cell adhesion, respectively, indicating their potential toward use in regenerative medicine.",
journal = "Life",
title = "Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles",
pages = "116",
volume = "13",
number = "1",
doi = "10.3390/life13010116",
url = "https://hdl.handle.net/21.15107/rcub_dais_13685"
}

Dorm, B. C., Iemma, M. R. C., Neto, B. D., Francisco, R. C. L., Dinić, I., Ignjatović, N., Marković, S., Vuković, M., Škapin, S., Trovatti, E.,& Mančić, L.. (2023). Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles. in Life, 13(1), 116.
https://doi.org/10.3390/life13010116
https://hdl.handle.net/21.15107/rcub_dais_13685

Dorm BC, Iemma MRC, Neto BD, Francisco RCL, Dinić I, Ignjatović N, Marković S, Vuković M, Škapin S, Trovatti E, Mančić L. Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles. in Life. 2023;13(1):116.
doi:10.3390/life13010116
https://hdl.handle.net/21.15107/rcub_dais_13685 .

Dorm, Bruna Carolina, Iemma, Mônica Rosas Costa, Neto, Benedito Domingos, Francisco, Rauany Cristina Lopes, Dinić, Ivana, Ignjatović, Nenad, Marković, Smilja, Vuković, Marina, Škapin, Srečo, Trovatti, Eliane, Mančić, Lidija, "Synthesis and Biological Properties of Alanine-Grafted Hydroxyapatite Nanoparticles" in Life, 13, no. 1 (2023):116,
https://doi.org/10.3390/life13010116 .,
https://hdl.handle.net/21.15107/rcub_dais_13685 .

TY  - CONF
AU  - Lopes, Rauany Cristina
AU  - Iemma, Mônica Rosas Costa
AU  - Montrezor, Luiz Henrique
AU  - Amaral, André Capaldo
AU  - Mančić, Lidija
AU  - Trovatti, Eliane
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15639
AB  - There are numerous researches on biomaterials, with the association of some natural and synthetic polymers and ceramic materials, for the development of hydrogels and bioinks, in an attempt to develop a biomaterial that is resistant, biocompatible and bioactive, which forms a bond with the host tissues and promotes tissue regeneration. The purpose of this work is to produce a bioink based on a hydrogel of chemically oxidized cellulose and bioglass (58S) for application in regenerative medicine. In this study, the chemically oxidized cellulose gel was obtained from a natural source of sugar cane bagasse, the material was washed, bleached, chemically treated with the TEMPO reagent and sonicated to obtain a viscous gel. Tertiary bioglass (58S) obtained by the sol-gel method was used because it showed better viability and cell proliferation. Chemically oxidized cellulose and bioglass (58S) were combined to form the composite. The results obtained were promising for characterizing the composite as a bioink. In this way, the rheological tests characterized the composite as a hydrogel. Subsequently, to form the bioink, bone cells (MG-63) were inserted inside the hydrogel. The results of the cell tests showed that after ten days the cells were still viable, as well as DAPI showing that the cells were inserted inside the material, characterizing it as a bioink and the Alizarin Red test showing the ability to form a mineralized matrix.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
T1  - Study of the properties of oxidized cellulose plus bioglass as a new bioink for application in regenerative medicine
SP  - 13
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_dais_15639
ER  - 

@conference{
author = "Lopes, Rauany Cristina and Iemma, Mônica Rosas Costa and Montrezor, Luiz Henrique and Amaral, André Capaldo and Mančić, Lidija and Trovatti, Eliane",
year = "2023",
abstract = "There are numerous researches on biomaterials, with the association of some natural and synthetic polymers and ceramic materials, for the development of hydrogels and bioinks, in an attempt to develop a biomaterial that is resistant, biocompatible and bioactive, which forms a bond with the host tissues and promotes tissue regeneration. The purpose of this work is to produce a bioink based on a hydrogel of chemically oxidized cellulose and bioglass (58S) for application in regenerative medicine. In this study, the chemically oxidized cellulose gel was obtained from a natural source of sugar cane bagasse, the material was washed, bleached, chemically treated with the TEMPO reagent and sonicated to obtain a viscous gel. Tertiary bioglass (58S) obtained by the sol-gel method was used because it showed better viability and cell proliferation. Chemically oxidized cellulose and bioglass (58S) were combined to form the composite. The results obtained were promising for characterizing the composite as a bioink. In this way, the rheological tests characterized the composite as a hydrogel. Subsequently, to form the bioink, bone cells (MG-63) were inserted inside the hydrogel. The results of the cell tests showed that after ten days the cells were still viable, as well as DAPI showing that the cells were inserted inside the material, characterizing it as a bioink and the Alizarin Red test showing the ability to form a mineralized matrix.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia",
title = "Study of the properties of oxidized cellulose plus bioglass as a new bioink for application in regenerative medicine",
pages = "13-13",
url = "https://hdl.handle.net/21.15107/rcub_dais_15639"
}

Lopes, R. C., Iemma, M. R. C., Montrezor, L. H., Amaral, A. C., Mančić, L.,& Trovatti, E.. (2023). Study of the properties of oxidized cellulose plus bioglass as a new bioink for application in regenerative medicine. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 13-13.
https://hdl.handle.net/21.15107/rcub_dais_15639

Lopes RC, Iemma MRC, Montrezor LH, Amaral AC, Mančić L, Trovatti E. Study of the properties of oxidized cellulose plus bioglass as a new bioink for application in regenerative medicine. in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia. 2023;:13-13.
https://hdl.handle.net/21.15107/rcub_dais_15639 .

Lopes, Rauany Cristina, Iemma, Mônica Rosas Costa, Montrezor, Luiz Henrique, Amaral, André Capaldo, Mančić, Lidija, Trovatti, Eliane, "Study of the properties of oxidized cellulose plus bioglass as a new bioink for application in regenerative medicine" in Program and the Book of abstracts / Twenty-first Young Researchers' Conference Materials Science and Engineering, November 29 – December 1, 2023, Belgrade, Serbia (2023):13-13,
https://hdl.handle.net/21.15107/rcub_dais_15639 .