TY  - JOUR
AU  - Radovanović, Mirjana
AU  - Jugović, Branimir
AU  - Gvozdenović, Milica M.
AU  - Jokić, Bojan
AU  - Grgur, Branimir
AU  - Bugarski, Branko
AU  - Knežević Jugović, Zorica
PY  - 2016
UR  - https://dais.sanu.ac.rs/123456789/16002
AB  - The immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline was studied. The support was characterized by field emission scanning electron microscopy (FESEM). The obtained magnetic particles were agglomerates of nanoparticles with sizes below 100 nm. The effects of various factors on immobilization, including time, the initial enzyme concentration, pH, and temperature, were examined. The optimum pH, temperature, and time for immobilization were established to be 7, 45°C and 75 min, respectively. The maximum amount of adsorbed α-amylase of 10/100 mg support was determined at the 5 mg/mL enzyme concentration. It appeared that α-amylase was stabilized in terms of pH and temperature by adsorption on magnetic particles coated with polyaniline. The good agreement of the equilibrium data with the Langmuir isotherm model confirmed the monolayer coverage of enzyme molecules on the surface of magnetic particles, and the maximum adsorption capacity was found to be 55.6/100 mg support at 25°C. The biocatalyst retained 55.5 ± 1.63% of its initial activity after nine cycles of reuse in starch hydrolysis at 60°C in a batch reactor. The immobilized enzyme also showed very good storage stability.
PB  - Hoboken, NJ : John Wiley & Sons
T2  - Starch
T1  - Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline
SP  - 427
EP  - 435
VL  - 68
IS  - 5-6
DO  - 10.1002/star.201500161
UR  - https://hdl.handle.net/21.15107/rcub_dais_16002
ER  - 

@article{
author = "Radovanović, Mirjana and Jugović, Branimir and Gvozdenović, Milica M. and Jokić, Bojan and Grgur, Branimir and Bugarski, Branko and Knežević Jugović, Zorica",
year = "2016",
abstract = "The immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline was studied. The support was characterized by field emission scanning electron microscopy (FESEM). The obtained magnetic particles were agglomerates of nanoparticles with sizes below 100 nm. The effects of various factors on immobilization, including time, the initial enzyme concentration, pH, and temperature, were examined. The optimum pH, temperature, and time for immobilization were established to be 7, 45°C and 75 min, respectively. The maximum amount of adsorbed α-amylase of 10/100 mg support was determined at the 5 mg/mL enzyme concentration. It appeared that α-amylase was stabilized in terms of pH and temperature by adsorption on magnetic particles coated with polyaniline. The good agreement of the equilibrium data with the Langmuir isotherm model confirmed the monolayer coverage of enzyme molecules on the surface of magnetic particles, and the maximum adsorption capacity was found to be 55.6/100 mg support at 25°C. The biocatalyst retained 55.5 ± 1.63% of its initial activity after nine cycles of reuse in starch hydrolysis at 60°C in a batch reactor. The immobilized enzyme also showed very good storage stability.",
publisher = "Hoboken, NJ : John Wiley & Sons",
journal = "Starch",
title = "Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline",
pages = "427-435",
volume = "68",
number = "5-6",
doi = "10.1002/star.201500161",
url = "https://hdl.handle.net/21.15107/rcub_dais_16002"
}

Radovanović, M., Jugović, B., Gvozdenović, M. M., Jokić, B., Grgur, B., Bugarski, B.,& Knežević Jugović, Z.. (2016). Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline. in Starch
Hoboken, NJ : John Wiley & Sons., 68(5-6), 427-435.
https://doi.org/10.1002/star.201500161
https://hdl.handle.net/21.15107/rcub_dais_16002

Radovanović M, Jugović B, Gvozdenović MM, Jokić B, Grgur B, Bugarski B, Knežević Jugović Z. Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline. in Starch. 2016;68(5-6):427-435.
doi:10.1002/star.201500161
https://hdl.handle.net/21.15107/rcub_dais_16002 .

Radovanović, Mirjana, Jugović, Branimir, Gvozdenović, Milica M., Jokić, Bojan, Grgur, Branimir, Bugarski, Branko, Knežević Jugović, Zorica, "Immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline" in Starch, 68, no. 5-6 (2016):427-435,
https://doi.org/10.1002/star.201500161 .,
https://hdl.handle.net/21.15107/rcub_dais_16002 .