TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Kuzmanović, Maja
AU  - Tomić, Nina
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Zheng, Kai
AU  - Stampfl, Juergen
AU  - Boccaccini, Aldo
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13579
AB  - Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022.
T2  - Journal of Biomaterials Applications
T1  - Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles
SP  - 1800
EP  - 1811
VL  - 36
IS  - 10
DO  - 10.1177/08853282211073731
UR  - https://hdl.handle.net/21.15107/rcub_dais_13579
ER  - 

@article{
author = "Stevanović, Magdalena and Filipović, Nenad and Kuzmanović, Maja and Tomić, Nina and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Stampfl, Juergen and Boccaccini, Aldo",
year = "2022",
abstract = "Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022.",
journal = "Journal of Biomaterials Applications",
title = "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles",
pages = "1800-1811",
volume = "36",
number = "10",
doi = "10.1177/08853282211073731",
url = "https://hdl.handle.net/21.15107/rcub_dais_13579"
}

Stevanović, M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J.,& Boccaccini, A.. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications, 36(10), 1800-1811.
https://doi.org/10.1177/08853282211073731
https://hdl.handle.net/21.15107/rcub_dais_13579

Stevanović M, Filipović N, Kuzmanović M, Tomić N, Ušjak D, Milenković M, Zheng K, Stampfl J, Boccaccini A. Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications. 2022;36(10):1800-1811.
doi:10.1177/08853282211073731
https://hdl.handle.net/21.15107/rcub_dais_13579 .

Stevanović, Magdalena, Filipović, Nenad, Kuzmanović, Maja, Tomić, Nina, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Stampfl, Juergen, Boccaccini, Aldo, "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles" in Journal of Biomaterials Applications, 36, no. 10 (2022):1800-1811,
https://doi.org/10.1177/08853282211073731 .,
https://hdl.handle.net/21.15107/rcub_dais_13579 .

TY  - JOUR
AU  - Filipović, Nenad
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Zheng, Kai
AU  - Liverani, Liliana
AU  - Boccaccini, Aldo
AU  - Stevanović, Magdalena
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/11631
AB  - Although selenium nanoparticles (SeNPs) have gained attention in the scientific community mostly through investigation of their anticancer activity, a great potential of this nanomaterial was recognized recently regarding its antimicrobial activity. The particle form, size, and surface chemistry have been recognized as crucial parameters determining the interaction of nanomaterials with biological entities. Furthermore, considering a narrow boundary between beneficial and toxic effects for selenium per se, it is clear that investigations of biomedical applications of SeNPs are very demanding and must be done with great precautions. The goal of this work is to evaluate the effects of SeNPs surface chemistry and structure on antimicrobial activity against several common bacterial strains, including Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Bacillus subtilis (ATCC 6633), and Kocuria rhizophila (ATCC 9341), as well as Escherichia coli (ATCC 8739), Salmonella Abony (NCTC 6017), Klebsiella pneumoniae (NCIMB 9111) and Pseudomonas aeruginosa (ATCC 9027), and the standard yeast strain Candida albicans (ATCC 10231). Three types of SeNPs were synthesized by chemical reduction approach using different stabilizers and reducing agents: (i) bovine serum albumin (BSA) + ascorbic acid, (ii) chitosan + ascorbic acid, and (iii) with glucose. A thorough physicochemical characterization of the obtained SeNPs was performed to determine the effects of varying synthesis parameters on their morphology, size, structure, and surface chemistry. All SeNPs were amorphous, with spherical morphology and size in the range 70–300 nm. However, the SeNPs obtained under different synthesis conditions, i.e. by using different stabilizers as well as reducing agents, exhibited different antimicrobial activity as well as cytotoxicity which are crucial for their applications. In this paper, the antimicrobial screening of the selected systems is presented, which was determined by the broth microdilution method, and inhibitory influence on the production of monomicrobial and dual-species biofilm was evaluated. The potential mechanism of action of different systems is proposed. Additionally, the cytotoxicity of SeNPs was examined on the MRC-5 cell line, in the same concentration interval as for antimicrobial testing. It was shown that formulation SeNPs-BSA expressed a significantly lower cytotoxic effect than the other two formulations.
PB  - Frontiers Media SA
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure
VL  - 8
DO  - 10.3389/fbioe.2020.624621
UR  - https://hdl.handle.net/21.15107/rcub_dais_11631
ER  - 

@article{
author = "Filipović, Nenad and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Liverani, Liliana and Boccaccini, Aldo and Stevanović, Magdalena",
year = "2021",
abstract = "Although selenium nanoparticles (SeNPs) have gained attention in the scientific community mostly through investigation of their anticancer activity, a great potential of this nanomaterial was recognized recently regarding its antimicrobial activity. The particle form, size, and surface chemistry have been recognized as crucial parameters determining the interaction of nanomaterials with biological entities. Furthermore, considering a narrow boundary between beneficial and toxic effects for selenium per se, it is clear that investigations of biomedical applications of SeNPs are very demanding and must be done with great precautions. The goal of this work is to evaluate the effects of SeNPs surface chemistry and structure on antimicrobial activity against several common bacterial strains, including Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Bacillus subtilis (ATCC 6633), and Kocuria rhizophila (ATCC 9341), as well as Escherichia coli (ATCC 8739), Salmonella Abony (NCTC 6017), Klebsiella pneumoniae (NCIMB 9111) and Pseudomonas aeruginosa (ATCC 9027), and the standard yeast strain Candida albicans (ATCC 10231). Three types of SeNPs were synthesized by chemical reduction approach using different stabilizers and reducing agents: (i) bovine serum albumin (BSA) + ascorbic acid, (ii) chitosan + ascorbic acid, and (iii) with glucose. A thorough physicochemical characterization of the obtained SeNPs was performed to determine the effects of varying synthesis parameters on their morphology, size, structure, and surface chemistry. All SeNPs were amorphous, with spherical morphology and size in the range 70–300 nm. However, the SeNPs obtained under different synthesis conditions, i.e. by using different stabilizers as well as reducing agents, exhibited different antimicrobial activity as well as cytotoxicity which are crucial for their applications. In this paper, the antimicrobial screening of the selected systems is presented, which was determined by the broth microdilution method, and inhibitory influence on the production of monomicrobial and dual-species biofilm was evaluated. The potential mechanism of action of different systems is proposed. Additionally, the cytotoxicity of SeNPs was examined on the MRC-5 cell line, in the same concentration interval as for antimicrobial testing. It was shown that formulation SeNPs-BSA expressed a significantly lower cytotoxic effect than the other two formulations.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure",
volume = "8",
doi = "10.3389/fbioe.2020.624621",
url = "https://hdl.handle.net/21.15107/rcub_dais_11631"
}

Filipović, N., Ušjak, D., Milenković, M., Zheng, K., Liverani, L., Boccaccini, A.,& Stevanović, M.. (2021). Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure. in Frontiers in Bioengineering and Biotechnology
Frontiers Media SA., 8.
https://doi.org/10.3389/fbioe.2020.624621
https://hdl.handle.net/21.15107/rcub_dais_11631

Filipović N, Ušjak D, Milenković M, Zheng K, Liverani L, Boccaccini A, Stevanović M. Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure. in Frontiers in Bioengineering and Biotechnology. 2021;8.
doi:10.3389/fbioe.2020.624621
https://hdl.handle.net/21.15107/rcub_dais_11631 .

Filipović, Nenad, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Liverani, Liliana, Boccaccini, Aldo, Stevanović, Magdalena, "Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure" in Frontiers in Bioengineering and Biotechnology, 8 (2021),
https://doi.org/10.3389/fbioe.2020.624621 .,
https://hdl.handle.net/21.15107/rcub_dais_11631 .

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Đurđević, Jelena
AU  - Lukić, Miodrag J.
AU  - Milenković, Marina
AU  - Boccaccini, Aldo
PY  - 2015
UR  - https://dais.sanu.ac.rs/123456789/4670
AB  - n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.
T2  - Colloids and Surfaces B: Biointerfaces
T1  - 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity
SP  - 208
EP  - 215
VL  - 132
DO  - 10.1016/j.colsurfb.2015.05.024
UR  - https://hdl.handle.net/21.15107/rcub_dais_4670
ER  - 

@article{
author = "Stevanović, Magdalena and Filipović, Nenad and Đurđević, Jelena and Lukić, Miodrag J. and Milenković, Marina and Boccaccini, Aldo",
year = "2015",
abstract = "n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity",
pages = "208-215",
volume = "132",
doi = "10.1016/j.colsurfb.2015.05.024",
url = "https://hdl.handle.net/21.15107/rcub_dais_4670"
}

Stevanović, M., Filipović, N., Đurđević, J., Lukić, M. J., Milenković, M.,& Boccaccini, A.. (2015). 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity. in Colloids and Surfaces B: Biointerfaces, 132, 208-215.
https://doi.org/10.1016/j.colsurfb.2015.05.024
https://hdl.handle.net/21.15107/rcub_dais_4670

Stevanović M, Filipović N, Đurđević J, Lukić MJ, Milenković M, Boccaccini A. 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity. in Colloids and Surfaces B: Biointerfaces. 2015;132:208-215.
doi:10.1016/j.colsurfb.2015.05.024
https://hdl.handle.net/21.15107/rcub_dais_4670 .

Stevanović, Magdalena, Filipović, Nenad, Đurđević, Jelena, Lukić, Miodrag J., Milenković, Marina, Boccaccini, Aldo, "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity" in Colloids and Surfaces B: Biointerfaces, 132 (2015):208-215,
https://doi.org/10.1016/j.colsurfb.2015.05.024 .,
https://hdl.handle.net/21.15107/rcub_dais_4670 .

TY  - CONF
AU  - Boccaccini, Aldo
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Lukić, Miodrag J.
AU  - Milenković, Marina
PY  - 2015
UR  - https://dais.sanu.ac.rs/123456789/856
AB  - In the bone tissue engineering field, there is growing interest in the application of bioglass scaffolds due to their bone bonding ability and osteoconductivity. However such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. enhanced bioactivity by incorporation of bioactive molecules or growth factors and effective antibacterial properties. A large number of epidemiological, preclinical, and clinical trials suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. Studies also provide evidence that Se intake may be necessary for bone health. Poly(lactide-co-glycolide) (PLGA) micro and nanoparticles are used for the controlled delivery of several classes of medicaments such as growth factors, antibiotics, antimicrobial agents etc. Uniform, stable, amorphous SeNps have been synthesized and additionally encapsulated within spherical PLGA particles (PLGA/SeNps). Bioglass scaffolds have been synthesized by foam replica method and additionally coated by SeNp or by PLGA with encapsulated SeNp. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, Bioglass®/SeNp and Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections.
PB  - Weimar : Deutsche Gesellschaft für Materialkunde e.V.
C3  - European Symposium and Exhibition on Biomaterials and Related Areas (Euro BioMAT), Weimar, Germany, 2015
T1  - Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles
UR  - https://hdl.handle.net/21.15107/rcub_dais_856
ER  - 

@conference{
author = "Boccaccini, Aldo and Stevanović, Magdalena and Filipović, Nenad and Veselinović, Ljiljana and Lukić, Miodrag J. and Milenković, Marina",
year = "2015",
abstract = "In the bone tissue engineering field, there is growing interest in the application of bioglass scaffolds due to their bone bonding ability and osteoconductivity. However such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. enhanced bioactivity by incorporation of bioactive molecules or growth factors and effective antibacterial properties. A large number of epidemiological, preclinical, and clinical trials suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. Studies also provide evidence that Se intake may be necessary for bone health. Poly(lactide-co-glycolide) (PLGA) micro and nanoparticles are used for the controlled delivery of several classes of medicaments such as growth factors, antibiotics, antimicrobial agents etc. Uniform, stable, amorphous SeNps have been synthesized and additionally encapsulated within spherical PLGA particles (PLGA/SeNps). Bioglass scaffolds have been synthesized by foam replica method and additionally coated by SeNp or by PLGA with encapsulated SeNp. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, Bioglass®/SeNp and Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections.",
publisher = "Weimar : Deutsche Gesellschaft für Materialkunde e.V.",
journal = "European Symposium and Exhibition on Biomaterials and Related Areas (Euro BioMAT), Weimar, Germany, 2015",
title = "Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles",
url = "https://hdl.handle.net/21.15107/rcub_dais_856"
}

Boccaccini, A., Stevanović, M., Filipović, N., Veselinović, L., Lukić, M. J.,& Milenković, M.. (2015). Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles. in European Symposium and Exhibition on Biomaterials and Related Areas (Euro BioMAT), Weimar, Germany, 2015
Weimar : Deutsche Gesellschaft für Materialkunde e.V...
https://hdl.handle.net/21.15107/rcub_dais_856

Boccaccini A, Stevanović M, Filipović N, Veselinović L, Lukić MJ, Milenković M. Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles. in European Symposium and Exhibition on Biomaterials and Related Areas (Euro BioMAT), Weimar, Germany, 2015. 2015;.
https://hdl.handle.net/21.15107/rcub_dais_856 .

Boccaccini, Aldo, Stevanović, Magdalena, Filipović, Nenad, Veselinović, Ljiljana, Lukić, Miodrag J., Milenković, Marina, "Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles" in European Symposium and Exhibition on Biomaterials and Related Areas (Euro BioMAT), Weimar, Germany, 2015 (2015),
https://hdl.handle.net/21.15107/rcub_dais_856 .

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Đurđević, Jelena
AU  - Lukić, Miodrag J.
AU  - Milenković, Marina
AU  - Boccaccini, Aldo
PY  - 2015
UR  - https://dais.sanu.ac.rs/123456789/758
AB  - n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.
T2  - Colloids and Surfaces B: Biointerfaces
T1  - 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity
SP  - 208
EP  - 215
VL  - 132
DO  - 10.1016/j.colsurfb.2015.05.024
UR  - https://hdl.handle.net/21.15107/rcub_dais_758
ER  - 

@article{
author = "Stevanović, Magdalena and Filipović, Nenad and Đurđević, Jelena and Lukić, Miodrag J. and Milenković, Marina and Boccaccini, Aldo",
year = "2015",
abstract = "n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity",
pages = "208-215",
volume = "132",
doi = "10.1016/j.colsurfb.2015.05.024",
url = "https://hdl.handle.net/21.15107/rcub_dais_758"
}

Stevanović, M., Filipović, N., Đurđević, J., Lukić, M. J., Milenković, M.,& Boccaccini, A.. (2015). 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity. in Colloids and Surfaces B: Biointerfaces, 132, 208-215.
https://doi.org/10.1016/j.colsurfb.2015.05.024
https://hdl.handle.net/21.15107/rcub_dais_758

Stevanović M, Filipović N, Đurđević J, Lukić MJ, Milenković M, Boccaccini A. 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity. in Colloids and Surfaces B: Biointerfaces. 2015;132:208-215.
doi:10.1016/j.colsurfb.2015.05.024
https://hdl.handle.net/21.15107/rcub_dais_758 .

Stevanović, Magdalena, Filipović, Nenad, Đurđević, Jelena, Lukić, Miodrag J., Milenković, Marina, Boccaccini, Aldo, "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity" in Colloids and Surfaces B: Biointerfaces, 132 (2015):208-215,
https://doi.org/10.1016/j.colsurfb.2015.05.024 .,
https://hdl.handle.net/21.15107/rcub_dais_758 .