TY  - CONF
AU  - Jovanović, Ivana
AU  - Petković, Marijana
AU  - Joksić, Gordana
AU  - Uskoković, Dragan
PY  - 2007
UR  - https://dais.sanu.ac.rs/123456789/6284
AB  - Particles generated of biodegradable material are extensively investigated as carriers
for sustained drug delivery. Studies have been mainly focused on the monitoring of the drug
release and of the rate of the particle degradation. However, the influence of the carrier particles
on the human immune cells was only rarely addressed. In this work, the influence of the
microparticles made of poly-D,L-lactide (PDLLA) on the function of human lymphocytes in a
three-day culture was investigated. PDLLA microparticles have been prepared by a modified
precipitation method and human lymphocytes were isolated from the blood of healthy volunteers
by a Ficoll – density gradient centrifugation. Lymphocyte proliferation test and the cytochalasin
B micronucleus test were used to assess the PDLLA particle effect on the lymphocytes. Results
showed that PDLLA particles did not influence on the proliferation of the human lymphocytes.
On the other hand, changes in the nuclei form, as well as nucleus buddings were observed.
Moreover, the appearance of micronuclei could be detected. All together, these results might
imply genotoxicity of the PDLLA particles, or some of the chemicals used for the particle
preparation/stabilization on the human lymphocytes.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Programme and The Book of Abstracts / Ninth Annual Conference YUCOMAT 2007, Herceg Novi, September 10-14, 2007
T1  - Genotoxicity of the poly-D,L-lactide microparticles on the human lymphocytes
SP  - 61
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_dais_6284
ER  - 

@conference{
author = "Jovanović, Ivana and Petković, Marijana and Joksić, Gordana and Uskoković, Dragan",
year = "2007",
abstract = "Particles generated of biodegradable material are extensively investigated as carriers
for sustained drug delivery. Studies have been mainly focused on the monitoring of the drug
release and of the rate of the particle degradation. However, the influence of the carrier particles
on the human immune cells was only rarely addressed. In this work, the influence of the
microparticles made of poly-D,L-lactide (PDLLA) on the function of human lymphocytes in a
three-day culture was investigated. PDLLA microparticles have been prepared by a modified
precipitation method and human lymphocytes were isolated from the blood of healthy volunteers
by a Ficoll – density gradient centrifugation. Lymphocyte proliferation test and the cytochalasin
B micronucleus test were used to assess the PDLLA particle effect on the lymphocytes. Results
showed that PDLLA particles did not influence on the proliferation of the human lymphocytes.
On the other hand, changes in the nuclei form, as well as nucleus buddings were observed.
Moreover, the appearance of micronuclei could be detected. All together, these results might
imply genotoxicity of the PDLLA particles, or some of the chemicals used for the particle
preparation/stabilization on the human lymphocytes.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Programme and The Book of Abstracts / Ninth Annual Conference YUCOMAT 2007, Herceg Novi, September 10-14, 2007",
title = "Genotoxicity of the poly-D,L-lactide microparticles on the human lymphocytes",
pages = "61-61",
url = "https://hdl.handle.net/21.15107/rcub_dais_6284"
}

Jovanović, I., Petković, M., Joksić, G.,& Uskoković, D.. (2007). Genotoxicity of the poly-D,L-lactide microparticles on the human lymphocytes. in Programme and The Book of Abstracts / Ninth Annual Conference YUCOMAT 2007, Herceg Novi, September 10-14, 2007
Belgrade : Institute of Technical Sciences of SASA., 61-61.
https://hdl.handle.net/21.15107/rcub_dais_6284

Jovanović I, Petković M, Joksić G, Uskoković D. Genotoxicity of the poly-D,L-lactide microparticles on the human lymphocytes. in Programme and The Book of Abstracts / Ninth Annual Conference YUCOMAT 2007, Herceg Novi, September 10-14, 2007. 2007;:61-61.
https://hdl.handle.net/21.15107/rcub_dais_6284 .

Jovanović, Ivana, Petković, Marijana, Joksić, Gordana, Uskoković, Dragan, "Genotoxicity of the poly-D,L-lactide microparticles on the human lymphocytes" in Programme and The Book of Abstracts / Ninth Annual Conference YUCOMAT 2007, Herceg Novi, September 10-14, 2007 (2007):61-61,
https://hdl.handle.net/21.15107/rcub_dais_6284 .