TY  - JOUR
AU  - Stanković, Ana
AU  - Sezen, Meltem
AU  - Milenković, Marina
AU  - Kaišarević, Sonja
AU  - Andrić, Nebojša
AU  - Stevanović, Magdalena
PY  - 2016
UR  - https://dais.sanu.ac.rs/123456789/901
AB  - Copolymer poly (DL-lactide-co-glycolide) (PLGA) is extensively investigated for various biomedical applications such as controlled drug delivery or carriers in the tissue engineering. In addition, zinc oxide (ZnO) is widely used in biomedicine especially for materials like dental composites, as a constituent of creams for the treatment of a variety of skin irritations, to enhance the antibacterial activity of different medicaments and so on. Uniform, spherical ZnO nanoparticles (nano-ZnO) have been synthesized via microwave synthesis method. In addition to obtaining nano-ZnO, a further aim was to examine their immobilization in the PLGA polymer matrix (PLGA/nano-ZnO) and this was done by a simple physicochemical solvent/nonsolvent method. The samples were characterized by X-ray diffraction, scanning electron microscopy, laser diffraction particle size analyzer, differential thermal analysis, and thermal gravimetric analysis. The synthesized PLGA/nano-ZnO particles are spherical, uniform, and with diameters below 1 µm. The influence of the different solvents and the drying methods during the synthesis was investigated too. The biocompatibility of the samples is discussed in terms of in vitro toxicity on human hepatoma HepG2 cells by application of MTT assay and the antimicrobial activity was evaluated by broth microdilution method against different groups of microorganisms (Gram-positive bacteria, Gram-negative bacteria, and yeast Candida albicans).
PB  - Hindawi
T2  - Journal of Nanomaterials
T1  - PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity
SP  - 9425289
VL  - 2016
DO  - 10.1155/2016/9425289
UR  - https://hdl.handle.net/21.15107/rcub_dais_901
ER  - 

@article{
author = "Stanković, Ana and Sezen, Meltem and Milenković, Marina and Kaišarević, Sonja and Andrić, Nebojša and Stevanović, Magdalena",
year = "2016",
abstract = "Copolymer poly (DL-lactide-co-glycolide) (PLGA) is extensively investigated for various biomedical applications such as controlled drug delivery or carriers in the tissue engineering. In addition, zinc oxide (ZnO) is widely used in biomedicine especially for materials like dental composites, as a constituent of creams for the treatment of a variety of skin irritations, to enhance the antibacterial activity of different medicaments and so on. Uniform, spherical ZnO nanoparticles (nano-ZnO) have been synthesized via microwave synthesis method. In addition to obtaining nano-ZnO, a further aim was to examine their immobilization in the PLGA polymer matrix (PLGA/nano-ZnO) and this was done by a simple physicochemical solvent/nonsolvent method. The samples were characterized by X-ray diffraction, scanning electron microscopy, laser diffraction particle size analyzer, differential thermal analysis, and thermal gravimetric analysis. The synthesized PLGA/nano-ZnO particles are spherical, uniform, and with diameters below 1 µm. The influence of the different solvents and the drying methods during the synthesis was investigated too. The biocompatibility of the samples is discussed in terms of in vitro toxicity on human hepatoma HepG2 cells by application of MTT assay and the antimicrobial activity was evaluated by broth microdilution method against different groups of microorganisms (Gram-positive bacteria, Gram-negative bacteria, and yeast Candida albicans).",
publisher = "Hindawi",
journal = "Journal of Nanomaterials",
title = "PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity",
pages = "9425289",
volume = "2016",
doi = "10.1155/2016/9425289",
url = "https://hdl.handle.net/21.15107/rcub_dais_901"
}

Stanković, A., Sezen, M., Milenković, M., Kaišarević, S., Andrić, N.,& Stevanović, M.. (2016). PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity. in Journal of Nanomaterials
Hindawi., 2016, 9425289.
https://doi.org/10.1155/2016/9425289
https://hdl.handle.net/21.15107/rcub_dais_901

Stanković A, Sezen M, Milenković M, Kaišarević S, Andrić N, Stevanović M. PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity. in Journal of Nanomaterials. 2016;2016:9425289.
doi:10.1155/2016/9425289
https://hdl.handle.net/21.15107/rcub_dais_901 .

Stanković, Ana, Sezen, Meltem, Milenković, Marina, Kaišarević, Sonja, Andrić, Nebojša, Stevanović, Magdalena, "PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity" in Journal of Nanomaterials, 2016 (2016):9425289,
https://doi.org/10.1155/2016/9425289 .,
https://hdl.handle.net/21.15107/rcub_dais_901 .

TY  - CONF
AU  - Filipović, Nenad
AU  - Lukić, Miodrag J.
AU  - Sengottuvelan, Abirami
AU  - Kaišarević, Sonja
PY  - 2015
UR  - https://dais.sanu.ac.rs/123456789/833
AB  - Tissue engineering (TE) is a growing field which provides helpful alternative strategies for conventional treatments in medicine. TE involves the smart combination of cells, biomolecules and engineered porous biomaterials in the form of 3D scaffolds. When it comes to bone regeneration the use of 3D scaffolds made of calcium phosphate is a well-known concept with a great potential. Here we present the foam replica method as a procedure suitable for producing highly porous scaffolds with the pore size in the range of 100-500 μm and the mean porosity of >90%. The obtained scaffolds were further coated with selenium nanoparticles (SeNp) and SeNp immobilized within poly(epsilon caprolactone) microspheres (PCL/Se). The purpose of such coating is based on the potential anticancer activity of SeNp as well as on their prolonged release from a biodegradable polymeric carrier. Scaffolds were characterized by X-ray diffraction, scanning electron microscopy, optical microscopy, thermogravimetric/differential thermal analysis (TGA-DTA) as well as Fourier transform infrared spectroscopy (FTIR). The cytotoxicity was determined employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and all the samples have shown good biocompatibility. Based on these preliminary results the obtained system can be considered as a candidate for the repair of bone lesions and damages.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia
T1  - Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method
SP  - 3
EP  - 3
UR  - https://hdl.handle.net/21.15107/rcub_dais_833
ER  - 

@conference{
author = "Filipović, Nenad and Lukić, Miodrag J. and Sengottuvelan, Abirami and Kaišarević, Sonja",
year = "2015",
abstract = "Tissue engineering (TE) is a growing field which provides helpful alternative strategies for conventional treatments in medicine. TE involves the smart combination of cells, biomolecules and engineered porous biomaterials in the form of 3D scaffolds. When it comes to bone regeneration the use of 3D scaffolds made of calcium phosphate is a well-known concept with a great potential. Here we present the foam replica method as a procedure suitable for producing highly porous scaffolds with the pore size in the range of 100-500 μm and the mean porosity of >90%. The obtained scaffolds were further coated with selenium nanoparticles (SeNp) and SeNp immobilized within poly(epsilon caprolactone) microspheres (PCL/Se). The purpose of such coating is based on the potential anticancer activity of SeNp as well as on their prolonged release from a biodegradable polymeric carrier. Scaffolds were characterized by X-ray diffraction, scanning electron microscopy, optical microscopy, thermogravimetric/differential thermal analysis (TGA-DTA) as well as Fourier transform infrared spectroscopy (FTIR). The cytotoxicity was determined employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and all the samples have shown good biocompatibility. Based on these preliminary results the obtained system can be considered as a candidate for the repair of bone lesions and damages.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia",
title = "Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method",
pages = "3-3",
url = "https://hdl.handle.net/21.15107/rcub_dais_833"
}

Filipović, N., Lukić, M. J., Sengottuvelan, A.,& Kaišarević, S.. (2015). Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method. in Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 3-3.
https://hdl.handle.net/21.15107/rcub_dais_833

Filipović N, Lukić MJ, Sengottuvelan A, Kaišarević S. Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method. in Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia. 2015;:3-3.
https://hdl.handle.net/21.15107/rcub_dais_833 .

Filipović, Nenad, Lukić, Miodrag J., Sengottuvelan, Abirami, Kaišarević, Sonja, "Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method" in Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia (2015):3-3,
https://hdl.handle.net/21.15107/rcub_dais_833 .