TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Sabetrasekh, Roya
AU  - Lyngstadaas, Staale Petter
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/653
AB  - Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
PB  - IOS Press
T2  - Bio-Medical Materials and Engineering
T1  - In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide
SP  - 1647
EP  - 1658
VL  - 24
IS  - 4
DO  - 10.3233/BME-140978
UR  - https://hdl.handle.net/21.15107/rcub_dais_653
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan",
year = "2014",
abstract = "Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.",
publisher = "IOS Press",
journal = "Bio-Medical Materials and Engineering",
title = "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide",
pages = "1647-1658",
volume = "24",
number = "4",
doi = "10.3233/BME-140978",
url = "https://hdl.handle.net/21.15107/rcub_dais_653"
}

Ignjatović, N., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D.. (2014). In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering
IOS Press., 24(4), 1647-1658.
https://doi.org/10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_653

Ignjatović N, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering. 2014;24(4):1647-1658.
doi:10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_653 .

Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Lyngstadaas, Staale Petter, Uskoković, Dragan, "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide" in Bio-Medical Materials and Engineering, 24, no. 4 (2014):1647-1658,
https://doi.org/10.3233/BME-140978 .,
https://hdl.handle.net/21.15107/rcub_dais_653 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Sabetrasekh, Roya
AU  - Lyngstadaas, Staale Petter
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/538
AB  - Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
T2  - Bio-Medical Materials and Engineering
T1  - In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide
SP  - 1647
EP  - 1658
VL  - 24
DO  - 10.3233/BME-140978
UR  - https://hdl.handle.net/21.15107/rcub_dais_538
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan",
year = "2014",
abstract = "Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.",
journal = "Bio-Medical Materials and Engineering",
title = "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide",
pages = "1647-1658",
volume = "24",
doi = "10.3233/BME-140978",
url = "https://hdl.handle.net/21.15107/rcub_dais_538"
}

Ignjatović, N., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D.. (2014). In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering, 24, 1647-1658.
https://doi.org/10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_538

Ignjatović N, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering. 2014;24:1647-1658.
doi:10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_538 .

Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Lyngstadaas, Staale Petter, Uskoković, Dragan, "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide" in Bio-Medical Materials and Engineering, 24 (2014):1647-1658,
https://doi.org/10.3233/BME-140978 .,
https://hdl.handle.net/21.15107/rcub_dais_538 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Sabetrasekh, Roya
AU  - Uskoković, Dragan
PY  - 2010
UR  - https://dais.sanu.ac.rs/123456789/179
AB  - The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nanoparticles contain calcium phosphate coated with bioresorbable polymer. The composite was analyzed by FT-IR, XRD and AFM methods. The average particle size of the nanocomposite ranges between 65 and 95 nm. Release profiles of tigecycline were obtained by UV–VIS spectroscopy in physiological solution at 37°C. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of lactate dehydrogenase (LDH) activity (under 37%) indicate low cytotoxicity level. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release. Having in mind the registered antibiotics concentration in other tissues, delivery systems with a higher tigecycline content show both local and systemic effects.
PB  - Springer US
T2  - Journal of Materials Science: Materials in Medicine
T1  - A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide
SP  - 231
EP  - 239
VL  - 21
IS  - 1
DO  - 10.1007/s10856-009-3854-6
UR  - https://hdl.handle.net/21.15107/rcub_dais_179
ER  - 

@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Uskoković, Dragan",
year = "2010",
abstract = "The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nanoparticles contain calcium phosphate coated with bioresorbable polymer. The composite was analyzed by FT-IR, XRD and AFM methods. The average particle size of the nanocomposite ranges between 65 and 95 nm. Release profiles of tigecycline were obtained by UV–VIS spectroscopy in physiological solution at 37°C. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of lactate dehydrogenase (LDH) activity (under 37%) indicate low cytotoxicity level. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release. Having in mind the registered antibiotics concentration in other tissues, delivery systems with a higher tigecycline content show both local and systemic effects.",
publisher = "Springer US",
journal = "Journal of Materials Science: Materials in Medicine",
title = "A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide",
pages = "231-239",
volume = "21",
number = "1",
doi = "10.1007/s10856-009-3854-6",
url = "https://hdl.handle.net/21.15107/rcub_dais_179"
}

Ignjatović, N., Ninkov, P., Sabetrasekh, R.,& Uskoković, D.. (2010). A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide. in Journal of Materials Science: Materials in Medicine
Springer US., 21(1), 231-239.
https://doi.org/10.1007/s10856-009-3854-6
https://hdl.handle.net/21.15107/rcub_dais_179

Ignjatović N, Ninkov P, Sabetrasekh R, Uskoković D. A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide. in Journal of Materials Science: Materials in Medicine. 2010;21(1):231-239.
doi:10.1007/s10856-009-3854-6
https://hdl.handle.net/21.15107/rcub_dais_179 .

Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Uskoković, Dragan, "A novel nano drug delivery system based on tigecycline-loaded calciumphosphate coated with poly-dl-lactide-co-glycolide" in Journal of Materials Science: Materials in Medicine, 21, no. 1 (2010):231-239,
https://doi.org/10.1007/s10856-009-3854-6 .,
https://hdl.handle.net/21.15107/rcub_dais_179 .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Sabetrasekh, Roya
AU  - Lyngstadaas, Staale Petter
AU  - Uskoković, Dragan
PY  - 2009
UR  - https://dais.sanu.ac.rs/123456789/4932
AB  - Calcium-phosphate/poly(dl-lactide-co-glycolide) (CP/PLGA) composite biomaterial in granular form showed a high potential in the reconstruction of bone tissue. Compared to pure polymers, the combination of CP with biodegradable polymers used in bone drug delivery systems shows certain advantages. Composite biomaterials in nano particulate (NPs) form may have significant advantages over those in micro- or submicro-particulate form. The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nano particles contain calcium phosphate coated with bioresorbable polymer and 0.6, 2 and 5wt% tigecycline. The composite was analyzed by FT-IR, XRD, HPLC and AFM methods. The average particle size of the nanocomposite increases with the augmentation of the part of antibiotics, and it ranges from 65 to 95 nm. Release profiles of tigecycline were obtained by UV-VIS spectroscopy in physiological solution at 37oC. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The in vitro cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of LDH activity (under 37%) indicate low cytotoxicity level. Inhibition of bacteria in aerobic and anaerobic conditions in vitro was analyzed after 1, 2 and 3 weeks. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009
T1  - In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline
SP  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_dais_4932
ER  - 

@conference{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan",
year = "2009",
abstract = "Calcium-phosphate/poly(dl-lactide-co-glycolide) (CP/PLGA) composite biomaterial in granular form showed a high potential in the reconstruction of bone tissue. Compared to pure polymers, the combination of CP with biodegradable polymers used in bone drug delivery systems shows certain advantages. Composite biomaterials in nano particulate (NPs) form may have significant advantages over those in micro- or submicro-particulate form. The purpose of the study presented in this paper has been to examine the possibility of the synthesis of a new nanoparticulate system for controlled and systemic drug delivery with double effect. In the first step, a drug is released from bioresorbable polymer; in the second stage, after resorption of the polymer, non-bioresorbable calcium phosphate remains the chief part of the particle and takes the role of a filler, filling a bone defect. The obtained tigecycline-loaded calcium-phosphate(CP)/poly(dl-lactide-co-glycolide)(PLGA) nano particles contain calcium phosphate coated with bioresorbable polymer and 0.6, 2 and 5wt% tigecycline. The composite was analyzed by FT-IR, XRD, HPLC and AFM methods. The average particle size of the nanocomposite increases with the augmentation of the part of antibiotics, and it ranges from 65 to 95 nm. Release profiles of tigecycline were obtained by UV-VIS spectroscopy in physiological solution at 37oC. Experimental results were analyzed using Peppas and Weibull mathematical models. Based on kinetic parameters, tigecycline release was defined as non-Fickian transport. The in vitro cytotoxicity of the nanocomposite was examined on standard cell lines of MC3T3-E1, in vitro. The obtained low values of LDH activity (under 37%) indicate low cytotoxicity level. Inhibition of bacteria in aerobic and anaerobic conditions in vitro was analyzed after 1, 2 and 3 weeks. The behaviour of the composite under real-life conditions was analyzed through implantation of the nanocomposite into living organisms, in vivo. The system with the lowest tigecycline content proved to be an adequate system for local and controlled release.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009",
title = "In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_dais_4932"
}

Ignjatović, N., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D.. (2009). In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline. in Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009
Belgrade : Institute of Technical Sciences of SASA., 71-71.
https://hdl.handle.net/21.15107/rcub_dais_4932

Ignjatović N, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline. in Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009. 2009;:71-71.
https://hdl.handle.net/21.15107/rcub_dais_4932 .

Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Lyngstadaas, Staale Petter, Uskoković, Dragan, "In vitro and in vivo performance of nanosized hydroxyapatite particles coated with poly-dl-lactide-co-glycolide as systems for drug delivery of tigecycline" in Programme and The Book of Abstracts / Eleventh Annual Conference YUCOMAT 2009, Herceg Novi, August 31 – September 4, 2009 (2009):71-71,
https://hdl.handle.net/21.15107/rcub_dais_4932 .