TY  - CONF
AU  - Ganić, Tea
AU  - Cvetković, Stefana
AU  - Vuletić, Stefana
AU  - Stević, Tatjana
AU  - Stevanović, Magdalena
AU  - Tomić, Nina
AU  - Nikolić, Biljana
AU  - Mitić Ćulafić, Dragana
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/16246
AB  - Cimet je jedna od najpoznatijih aromatičnih biljaka, koje je najčešće korišćena kao začin. Prepoznat je kao potencijalni terapeutik u narodnoj medicini, zbog čega se dugo koristi za lečenje različitih infekcija. Etarska ulja su sekundarni metaboliti koje sintetišu aromatične biljke. Za etarsko ulje cimeta (CEO) su definisane različite biološke aktivnosti, međutim fizičkohemijske karakteristike ulja su slaba rastvorljivost u vodi i laka isparljivost, što ograničava njihovu primenu. Iz tog razloga, formulacija emulzija etarskih ulja predstavlja dobro rešenje za prevazilaženje problema njihove primene. Iako se CEO smatra bezbednim za upotrebu, rezultati istraživanja variraju, ukazujući na neophodnost daljih toksikoloških istraživanja. Cilj ovog rada je bila komparativna analiza biološke aktivnosti i in vitro toksičnosti CEO i njegove emulzije (EM). Antioksidativni potencijal je ispitivan DPPH testom. MTT test je primenjen u cilju procene citotoksičnog efekta CEO i EM na fibroblastima pluća fetusa (MRC–5). Na istoj ćelijskoj liniji, ispitivan je nivo oštećenja molekula DNK, primenom alkalnog komet testa. Na osnovu rezltata DPPH testa, IC50 vrednost za CEO je bila 320 μg/mL, dok je za EM bila 54 μg/mL. Na ćelijskoj liniji MRC–5, IC50 vrednosti dobijene MTT testom su iznosile 0,13 mg/mL za CEO i 0,025 mg/mL za EM. CEO i EM nisu pokazali genotoksični potencijal. Uzimajući sve prethodno navedeno u obzir, CEO i EM, kao jaki antioksidanti koji pokazuju odsustvo genotoksičnosti i slabu citotoksičnost.
AB  - Cinnamon is among most popular aromatic plants, generally used as a spice. Over the centuries it was recognized as a potential therapeutic for different kind of infections. Essential oils are aromatic plants’ secondary metabolites. Cinnamon essential oil (CEO) possess many favorable biological activities, but its physicochemical properties, as an oil, are poor solubility in water and high volatility, which limits its application. For that reason, preparation of essential oils’ emulsions seems to be solution to overcome applicability problems. Even though CEO is one of the oils recognized as safe to be used, the results from different studies vary, indicating necessity to further study its toxicological properties. The aim of this study was to comparatively analyze CEO and its emulsion (EM) biological activity and in vitro toxicity. Antioxidative potential was investigated using DPPH assay. The MTT assay was applied for the estimation of cytotoxic effects of CEO and EM on fetal fibroblasts (MRC-5) cell line. On the same cell line, level of DNA damage was evaluated, using alkaline comet assay. According to the DPPH assay results, IC50 value for CEO was 320 μg/mL, for EM was 54 μg/mL. On MRC-5 cell line, IC50 values were 0.13 mg/mL for CEO and 0.025 mg/mL for EM. Both CEO and EM didn’t exhibit potential to induce DNA damage. Taking all above mention into account, as strong antioxidants without genotoxicity and with weak cytotoxicity, CEO and EM could be considered as good candidates for further investigation of biological activities and potential for human use.
PB  - Beograd : Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book
T1  - Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije
T1  - Evaluation of antioxidative, cytotoxic and genotoxic effect of cinnamon essential oil and its emulsion
SP  - 152
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_dais_16246
ER  - 

@conference{
author = "Ganić, Tea and Cvetković, Stefana and Vuletić, Stefana and Stević, Tatjana and Stevanović, Magdalena and Tomić, Nina and Nikolić, Biljana and Mitić Ćulafić, Dragana",
year = "2023",
abstract = "Cimet je jedna od najpoznatijih aromatičnih biljaka, koje je najčešće korišćena kao začin. Prepoznat je kao potencijalni terapeutik u narodnoj medicini, zbog čega se dugo koristi za lečenje različitih infekcija. Etarska ulja su sekundarni metaboliti koje sintetišu aromatične biljke. Za etarsko ulje cimeta (CEO) su definisane različite biološke aktivnosti, međutim fizičkohemijske karakteristike ulja su slaba rastvorljivost u vodi i laka isparljivost, što ograničava njihovu primenu. Iz tog razloga, formulacija emulzija etarskih ulja predstavlja dobro rešenje za prevazilaženje problema njihove primene. Iako se CEO smatra bezbednim za upotrebu, rezultati istraživanja variraju, ukazujući na neophodnost daljih toksikoloških istraživanja. Cilj ovog rada je bila komparativna analiza biološke aktivnosti i in vitro toksičnosti CEO i njegove emulzije (EM). Antioksidativni potencijal je ispitivan DPPH testom. MTT test je primenjen u cilju procene citotoksičnog efekta CEO i EM na fibroblastima pluća fetusa (MRC–5). Na istoj ćelijskoj liniji, ispitivan je nivo oštećenja molekula DNK, primenom alkalnog komet testa. Na osnovu rezltata DPPH testa, IC50 vrednost za CEO je bila 320 μg/mL, dok je za EM bila 54 μg/mL. Na ćelijskoj liniji MRC–5, IC50 vrednosti dobijene MTT testom su iznosile 0,13 mg/mL za CEO i 0,025 mg/mL za EM. CEO i EM nisu pokazali genotoksični potencijal. Uzimajući sve prethodno navedeno u obzir, CEO i EM, kao jaki antioksidanti koji pokazuju odsustvo genotoksičnosti i slabu citotoksičnost., Cinnamon is among most popular aromatic plants, generally used as a spice. Over the centuries it was recognized as a potential therapeutic for different kind of infections. Essential oils are aromatic plants’ secondary metabolites. Cinnamon essential oil (CEO) possess many favorable biological activities, but its physicochemical properties, as an oil, are poor solubility in water and high volatility, which limits its application. For that reason, preparation of essential oils’ emulsions seems to be solution to overcome applicability problems. Even though CEO is one of the oils recognized as safe to be used, the results from different studies vary, indicating necessity to further study its toxicological properties. The aim of this study was to comparatively analyze CEO and its emulsion (EM) biological activity and in vitro toxicity. Antioxidative potential was investigated using DPPH assay. The MTT assay was applied for the estimation of cytotoxic effects of CEO and EM on fetal fibroblasts (MRC-5) cell line. On the same cell line, level of DNA damage was evaluated, using alkaline comet assay. According to the DPPH assay results, IC50 value for CEO was 320 μg/mL, for EM was 54 μg/mL. On MRC-5 cell line, IC50 values were 0.13 mg/mL for CEO and 0.025 mg/mL for EM. Both CEO and EM didn’t exhibit potential to induce DNA damage. Taking all above mention into account, as strong antioxidants without genotoxicity and with weak cytotoxicity, CEO and EM could be considered as good candidates for further investigation of biological activities and potential for human use.",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book",
title = "Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije, Evaluation of antioxidative, cytotoxic and genotoxic effect of cinnamon essential oil and its emulsion",
pages = "152-153",
url = "https://hdl.handle.net/21.15107/rcub_dais_16246"
}

Ganić, T., Cvetković, S., Vuletić, S., Stević, T., Stevanović, M., Tomić, N., Nikolić, B.,& Mitić Ćulafić, D.. (2023). Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book
Beograd : Udruženje toksikologa Srbije., 152-153.
https://hdl.handle.net/21.15107/rcub_dais_16246

Ganić T, Cvetković S, Vuletić S, Stević T, Stevanović M, Tomić N, Nikolić B, Mitić Ćulafić D. Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book. 2023;:152-153.
https://hdl.handle.net/21.15107/rcub_dais_16246 .

Ganić, Tea, Cvetković, Stefana, Vuletić, Stefana, Stević, Tatjana, Stevanović, Magdalena, Tomić, Nina, Nikolić, Biljana, Mitić Ćulafić, Dragana, "Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book (2023):152-153,
https://hdl.handle.net/21.15107/rcub_dais_16246 .

TY  - CONF
AU  - Vuletić, Stefana
AU  - Cvetković, Stefana
AU  - Tomić, Nina
AU  - Ganić, Tea
AU  - Tomić, Sergej
AU  - Bekić, Marina
AU  - Nikolić, Biljana
AU  - Mitić Ćulafić, Dragana
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/16247
AB  - Hepatocelularni i kolorektalni karcinom se sve ćešće javlja i ima visoku stopu smrtnosti. Postojanje aktivnih mehanizama hemorezistencije otežava korisnost hemoterapije. Upravo je pojava hemorezistencije podstakla opsežna istraživanja za razvoj novih terapeutskih agenasa i strategija za lečenje ovih vrsta karcinoma. Prirodni proizvodi biljnog porekla (sekundarni metaboliti) su bogat izvor potencijalnih lekova. Zbog toga smo u istraživanju usmerili pažnju na etil-acetatni ekstrakt biljke Frangula alnus (FA) i njegovu dominantnu komponentu emodin (E) i ispitali njihovo antikancerski potencijal na ćelijama hepatocelularnog (HepG2) i kolorektalnog (HCT116) karcinoma i citotoksičnost na normalnim MRC -5 ćelijama. Pokazan je snažan antioksidativni potencijal ekstrakta (DPPH i TBA test). Citotoksičnost ispitana MTT testom pokazala je jaku toksičnost obe supstance na ćelije HepG2 i HCT116, ali bez uticaja na MRC-5 ćelije. Dalje je analiza ćelijskog ciklusa pokazala da su i FA i E izazvali zaustavljanje u G1 fazi i blago nakupljanje ćelija u G2/M fazi. Pored toga, obe supstance su uvele ćelije u apoptozu i nekrozu i uticale su na mitohondrijalni membranski potencijal. Dalje, izazvali su značajan genotoksični efekat u kometnom testu na svim ćelijskim linijama. Može se zaključiti da su FA i E dobri kandidati za nove antikancerogene agense prirodnog porekla. Međutim, neophodna su dodatna istraživanja, posebno kada je u pitanju citotoksična aktivnost i bezbednost primene ovih supstanci.
AB  - Hepatocellular and colorectal carcinoma is swiftly increasing alongside a high mortality rate. The existence of active mechanisms of chemoresistance hampers the usefulness of chemotherapy. The emergence of chemoresistance encouraged extensive research to develop new therapeutic agents and strategies for the treatment of these types of cancer. Natural products of plant origin (secondary metabolites) have been the most successful source of potential drug leads. Therefore, we turned our attention to the Frangula alnus ethyl-acetate extract (FA) and its dominant constituent emodin (E) and explored them for their anticancer potential on hepatocellular (HepG2) and colorectal (HCT116) carcinoma cells, and cytotoxicity on normal MRC-5 cells. Strong antioxidant potential of the extract was demonstrated (DPPH and TBA test). Cytotoxicity investigated by the MTT assay showed strong cell toxicity of both substances on HepG2 and HCT116 cells, but without affecting MRC-5 cells. Next, the analysis of cell cycles exhibited that both FA and E induced arrest in the G1 phase and slight accumulation of cells in the G2/M phase. In addition, both substances introduced cells into apoptosis and necrosis and modulated mitochondrial membrane potential. Further on, they caused significant genotoxic effect in comet assay applied in all cell lines. It can be concluded that FA and E are good candidates for new anticancer agents of natural origin. However, additional studies are necessary, especially when it comes to the cytotoxic activity and safety of the application of these substances.
PB  - Beograd : Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023
T1  - Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi
T1  - Frangula alnus extract and emodin as potential anticancer agents
SP  - 148
EP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_dais_16247
ER  - 

@conference{
author = "Vuletić, Stefana and Cvetković, Stefana and Tomić, Nina and Ganić, Tea and Tomić, Sergej and Bekić, Marina and Nikolić, Biljana and Mitić Ćulafić, Dragana",
year = "2023",
abstract = "Hepatocelularni i kolorektalni karcinom se sve ćešće javlja i ima visoku stopu smrtnosti. Postojanje aktivnih mehanizama hemorezistencije otežava korisnost hemoterapije. Upravo je pojava hemorezistencije podstakla opsežna istraživanja za razvoj novih terapeutskih agenasa i strategija za lečenje ovih vrsta karcinoma. Prirodni proizvodi biljnog porekla (sekundarni metaboliti) su bogat izvor potencijalnih lekova. Zbog toga smo u istraživanju usmerili pažnju na etil-acetatni ekstrakt biljke Frangula alnus (FA) i njegovu dominantnu komponentu emodin (E) i ispitali njihovo antikancerski potencijal na ćelijama hepatocelularnog (HepG2) i kolorektalnog (HCT116) karcinoma i citotoksičnost na normalnim MRC -5 ćelijama. Pokazan je snažan antioksidativni potencijal ekstrakta (DPPH i TBA test). Citotoksičnost ispitana MTT testom pokazala je jaku toksičnost obe supstance na ćelije HepG2 i HCT116, ali bez uticaja na MRC-5 ćelije. Dalje je analiza ćelijskog ciklusa pokazala da su i FA i E izazvali zaustavljanje u G1 fazi i blago nakupljanje ćelija u G2/M fazi. Pored toga, obe supstance su uvele ćelije u apoptozu i nekrozu i uticale su na mitohondrijalni membranski potencijal. Dalje, izazvali su značajan genotoksični efekat u kometnom testu na svim ćelijskim linijama. Može se zaključiti da su FA i E dobri kandidati za nove antikancerogene agense prirodnog porekla. Međutim, neophodna su dodatna istraživanja, posebno kada je u pitanju citotoksična aktivnost i bezbednost primene ovih supstanci., Hepatocellular and colorectal carcinoma is swiftly increasing alongside a high mortality rate. The existence of active mechanisms of chemoresistance hampers the usefulness of chemotherapy. The emergence of chemoresistance encouraged extensive research to develop new therapeutic agents and strategies for the treatment of these types of cancer. Natural products of plant origin (secondary metabolites) have been the most successful source of potential drug leads. Therefore, we turned our attention to the Frangula alnus ethyl-acetate extract (FA) and its dominant constituent emodin (E) and explored them for their anticancer potential on hepatocellular (HepG2) and colorectal (HCT116) carcinoma cells, and cytotoxicity on normal MRC-5 cells. Strong antioxidant potential of the extract was demonstrated (DPPH and TBA test). Cytotoxicity investigated by the MTT assay showed strong cell toxicity of both substances on HepG2 and HCT116 cells, but without affecting MRC-5 cells. Next, the analysis of cell cycles exhibited that both FA and E induced arrest in the G1 phase and slight accumulation of cells in the G2/M phase. In addition, both substances introduced cells into apoptosis and necrosis and modulated mitochondrial membrane potential. Further on, they caused significant genotoxic effect in comet assay applied in all cell lines. It can be concluded that FA and E are good candidates for new anticancer agents of natural origin. However, additional studies are necessary, especially when it comes to the cytotoxic activity and safety of the application of these substances.",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023",
title = "Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi, Frangula alnus extract and emodin as potential anticancer agents",
pages = "148-149",
url = "https://hdl.handle.net/21.15107/rcub_dais_16247"
}

Vuletić, S., Cvetković, S., Tomić, N., Ganić, T., Tomić, S., Bekić, M., Nikolić, B.,& Mitić Ćulafić, D.. (2023). Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023
Beograd : Udruženje toksikologa Srbije., 148-149.
https://hdl.handle.net/21.15107/rcub_dais_16247

Vuletić S, Cvetković S, Tomić N, Ganić T, Tomić S, Bekić M, Nikolić B, Mitić Ćulafić D. Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023. 2023;:148-149.
https://hdl.handle.net/21.15107/rcub_dais_16247 .

Vuletić, Stefana, Cvetković, Stefana, Tomić, Nina, Ganić, Tea, Tomić, Sergej, Bekić, Marina, Nikolić, Biljana, Mitić Ćulafić, Dragana, "Frangula alnus ekstrakt i emodin kao potencijalni antikancerski agensi" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book, 2023 (2023):148-149,
https://hdl.handle.net/21.15107/rcub_dais_16247 .

TY  - JOUR
AU  - Ganić, Tea
AU  - Vuletić, Stefana
AU  - Nikolić, Biljana
AU  - Stevanović, Magdalena
AU  - Kuzmanović, Maja
AU  - Kekić, Dušan
AU  - Đurović, Saša
AU  - Cvetković, Stefana
AU  - Mitić-Ćulafić, Dragana
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13509
AB  - Acinetobacter baumannii is an emerging nosocomial pathogen resistant to a wide spectrum of antibiotics, with great potential to form a biofilm, which further aggravates treatment of infections caused by it. Therefore, searching for new potent agents that are efficient against A. baumannii seems to be a necessity. One of them, which has already been proven to possess a wide spectrum of biological activities, including antimicrobial effect, is cinnamon essential oil. Still, further increase of antibacterial efficacy and improvement of bioavailability of cinnamon oil is possible by emulsification process. The aim of this study was comparative analysis of cinnamon essential oil and its emulsion against biofilm forming A. baumannii clinical isolates. Furthermore, the investigation of toxicological aspects of possible applications of essential oil and emulsion was done as well. Gas chromatography–mass spectrometry of essential oil indicated trans-cinnamaldehyde as the most abundant component. The cinnamon emulsion was synthesized from cinnamon essential oil by combining modified low- and high- energy methods. Synthesized emulsion was characterized with Fourier-transform infrared spectroscopy and photon correlation spectroscopy. Both substances exhibited significant antibacterial (minimal inhibitory concentrations in the range 0.125–0.5 mg/ml) and antibiofilm effects (inhibitions of formation and reduction of pre-formed biofilm were 47–81 and 30–62%, respectively). Compared to essential oil, the efficacy of emulsion was even stronger considering the small share of pure oil (20%) in the emulsion. The result of biofilm eradication assay was confirmed by scanning electron microscopy. Even though the cytotoxicity was high especially for the emulsion, genotoxicity was not determined. In conclusion, strong antibacterial/antibiofilm effect against A. baumannii of the cinnamon essential oil and the fact that emulsification even potentiated the activity, seems to be of great significance. Observed cytotoxicity implicated that further analysis is needed in order to clearly determine active principles being responsible for obtained antibacterial/antibiofilm and cytotoxic properties. Copyright © 2022 Ganić, Vuletić, Nikolić, Stevanović, Kuzmanović, Kekić, Đurović, Cvetković and Mitić-Ćulafić.
T2  - Frontiers in Microbiology
T1  - Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii
VL  - 13
DO  - 10.3389/fmicb.2022.989667
UR  - https://hdl.handle.net/21.15107/rcub_dais_13509
ER  - 

@article{
author = "Ganić, Tea and Vuletić, Stefana and Nikolić, Biljana and Stevanović, Magdalena and Kuzmanović, Maja and Kekić, Dušan and Đurović, Saša and Cvetković, Stefana and Mitić-Ćulafić, Dragana",
year = "2022",
abstract = "Acinetobacter baumannii is an emerging nosocomial pathogen resistant to a wide spectrum of antibiotics, with great potential to form a biofilm, which further aggravates treatment of infections caused by it. Therefore, searching for new potent agents that are efficient against A. baumannii seems to be a necessity. One of them, which has already been proven to possess a wide spectrum of biological activities, including antimicrobial effect, is cinnamon essential oil. Still, further increase of antibacterial efficacy and improvement of bioavailability of cinnamon oil is possible by emulsification process. The aim of this study was comparative analysis of cinnamon essential oil and its emulsion against biofilm forming A. baumannii clinical isolates. Furthermore, the investigation of toxicological aspects of possible applications of essential oil and emulsion was done as well. Gas chromatography–mass spectrometry of essential oil indicated trans-cinnamaldehyde as the most abundant component. The cinnamon emulsion was synthesized from cinnamon essential oil by combining modified low- and high- energy methods. Synthesized emulsion was characterized with Fourier-transform infrared spectroscopy and photon correlation spectroscopy. Both substances exhibited significant antibacterial (minimal inhibitory concentrations in the range 0.125–0.5 mg/ml) and antibiofilm effects (inhibitions of formation and reduction of pre-formed biofilm were 47–81 and 30–62%, respectively). Compared to essential oil, the efficacy of emulsion was even stronger considering the small share of pure oil (20%) in the emulsion. The result of biofilm eradication assay was confirmed by scanning electron microscopy. Even though the cytotoxicity was high especially for the emulsion, genotoxicity was not determined. In conclusion, strong antibacterial/antibiofilm effect against A. baumannii of the cinnamon essential oil and the fact that emulsification even potentiated the activity, seems to be of great significance. Observed cytotoxicity implicated that further analysis is needed in order to clearly determine active principles being responsible for obtained antibacterial/antibiofilm and cytotoxic properties. Copyright © 2022 Ganić, Vuletić, Nikolić, Stevanović, Kuzmanović, Kekić, Đurović, Cvetković and Mitić-Ćulafić.",
journal = "Frontiers in Microbiology",
title = "Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii",
volume = "13",
doi = "10.3389/fmicb.2022.989667",
url = "https://hdl.handle.net/21.15107/rcub_dais_13509"
}

Ganić, T., Vuletić, S., Nikolić, B., Stevanović, M., Kuzmanović, M., Kekić, D., Đurović, S., Cvetković, S.,& Mitić-Ćulafić, D.. (2022). Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii. in Frontiers in Microbiology, 13.
https://doi.org/10.3389/fmicb.2022.989667
https://hdl.handle.net/21.15107/rcub_dais_13509

Ganić T, Vuletić S, Nikolić B, Stevanović M, Kuzmanović M, Kekić D, Đurović S, Cvetković S, Mitić-Ćulafić D. Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii. in Frontiers in Microbiology. 2022;13.
doi:10.3389/fmicb.2022.989667
https://hdl.handle.net/21.15107/rcub_dais_13509 .

Ganić, Tea, Vuletić, Stefana, Nikolić, Biljana, Stevanović, Magdalena, Kuzmanović, Maja, Kekić, Dušan, Đurović, Saša, Cvetković, Stefana, Mitić-Ćulafić, Dragana, "Cinnamon essential oil and its emulsion as efficient antibiofilm agents to combat Acinetobacter baumannii" in Frontiers in Microbiology, 13 (2022),
https://doi.org/10.3389/fmicb.2022.989667 .,
https://hdl.handle.net/21.15107/rcub_dais_13509 .

TY  - CONF
AU  - Ganić, Tea
AU  - Vuletić, Stefana
AU  - Stevanović, Magdalena
AU  - Kuzmanović, Maja
AU  - Cvetković, Stefana
AU  - Nikolić, Biljana
AU  - Đurović, Saša
AU  - Kekić, Dušan
AU  - Mitić-Ćulafić, Dragana
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13610
AB  - Objective: Acinetobacter baumannii is a pathogenic species which presents a danger to healthcare facilities due to the rapid spread of antibiotic resistance. Plants are currently being explored as a potential source of bioactive compounds that could be used to combat infectious diseases. Cinnamon is used as a food spice, but essential oil has been proven for antimicrobial activity. Due to reduced stability and solubility of essential oil, nanoemulsion (NE) synthesis could provide stronger antimicrobial effects. Investigation and comparison of antimicrobial activity of cinnamon (Cinnamomum zeylanicum L.) bark essential oil (EO) and NE on the A. baumannii ATCC19606 and clinical isolates. Effect of EO and NE on biofilm formation and biofilm eradication.
Methods: GC/MS was performed in order to determine chemical composition of commercially purchased EO (P0125285, Frey + Lau, GmbH, Henstedt-Ulzburg, Germany). Droplet/particle size and polydispersity index of NE was determined by photon correlation spectroscopy (PCS). Minimal inhibitory concentration of EO and NE were defined using MIC assay. Effects of EO and NE were also examined on biofilm formation and eradication. Crystal violet staining was used for biofilm biomass quantification.
Results: GC/MS analysis determined that the most common compound was trans-Cinnamaldehyde (61.9%). NE droplet/particles had multimodal distribution, shown by PCS. MIC values for EO were in range 0.25mg/mL — 0.5mg/mL, and for NE 0.125mg/mL — 0.25mg/mL. Both tested substances showed good effect on biofilm eradication, and destroyed biofilm biomass up to 64%, whilst the inhibition of biofilm formation was up to 70%.
Conclusion: Taking all the results into account, it is a good start for further investigations of both EO and NE as a potential antimicrobial agent. Deeper knowledge about the mechanisms of actions of both of these tested substances could help us to understand the best and the most effective way to use them, in order to combat against Acinetobacter baumannii.
PB  - Wroclaw : College of Physiotherapy
C3  - Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022
T1  - Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_dais_13610
ER  - 

@conference{
author = "Ganić, Tea and Vuletić, Stefana and Stevanović, Magdalena and Kuzmanović, Maja and Cvetković, Stefana and Nikolić, Biljana and Đurović, Saša and Kekić, Dušan and Mitić-Ćulafić, Dragana",
year = "2022",
abstract = "Objective: Acinetobacter baumannii is a pathogenic species which presents a danger to healthcare facilities due to the rapid spread of antibiotic resistance. Plants are currently being explored as a potential source of bioactive compounds that could be used to combat infectious diseases. Cinnamon is used as a food spice, but essential oil has been proven for antimicrobial activity. Due to reduced stability and solubility of essential oil, nanoemulsion (NE) synthesis could provide stronger antimicrobial effects. Investigation and comparison of antimicrobial activity of cinnamon (Cinnamomum zeylanicum L.) bark essential oil (EO) and NE on the A. baumannii ATCC19606 and clinical isolates. Effect of EO and NE on biofilm formation and biofilm eradication.
Methods: GC/MS was performed in order to determine chemical composition of commercially purchased EO (P0125285, Frey + Lau, GmbH, Henstedt-Ulzburg, Germany). Droplet/particle size and polydispersity index of NE was determined by photon correlation spectroscopy (PCS). Minimal inhibitory concentration of EO and NE were defined using MIC assay. Effects of EO and NE were also examined on biofilm formation and eradication. Crystal violet staining was used for biofilm biomass quantification.
Results: GC/MS analysis determined that the most common compound was trans-Cinnamaldehyde (61.9%). NE droplet/particles had multimodal distribution, shown by PCS. MIC values for EO were in range 0.25mg/mL — 0.5mg/mL, and for NE 0.125mg/mL — 0.25mg/mL. Both tested substances showed good effect on biofilm eradication, and destroyed biofilm biomass up to 64%, whilst the inhibition of biofilm formation was up to 70%.
Conclusion: Taking all the results into account, it is a good start for further investigations of both EO and NE as a potential antimicrobial agent. Deeper knowledge about the mechanisms of actions of both of these tested substances could help us to understand the best and the most effective way to use them, in order to combat against Acinetobacter baumannii.",
publisher = "Wroclaw : College of Physiotherapy",
journal = "Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022",
title = "Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_dais_13610"
}

Ganić, T., Vuletić, S., Stevanović, M., Kuzmanović, M., Cvetković, S., Nikolić, B., Đurović, S., Kekić, D.,& Mitić-Ćulafić, D.. (2022). Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates. in Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022
Wroclaw : College of Physiotherapy., 85-85.
https://hdl.handle.net/21.15107/rcub_dais_13610

Ganić T, Vuletić S, Stevanović M, Kuzmanović M, Cvetković S, Nikolić B, Đurović S, Kekić D, Mitić-Ćulafić D. Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates. in Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022. 2022;:85-85.
https://hdl.handle.net/21.15107/rcub_dais_13610 .

Ganić, Tea, Vuletić, Stefana, Stevanović, Magdalena, Kuzmanović, Maja, Cvetković, Stefana, Nikolić, Biljana, Đurović, Saša, Kekić, Dušan, Mitić-Ćulafić, Dragana, "Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates" in Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022 (2022):85-85,
https://hdl.handle.net/21.15107/rcub_dais_13610 .

TY  - CONF
AU  - Ганић, Теа
AU  - Вулетић, Стефана
AU  - Цветковић, Стефана
AU  - Стевановић, Магдалена
AU  - Кузмановић, Маја
AU  - Ђуровић, Саша
AU  - Николић, Биљана
AU  - Митић-Ћулафић, Драгана
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13611
AB  - Антимикробна резистенција представља велики проблем за јавно здравље због ограничених терапијских могућности. Acinetobacter baumannii је опортунистички патоген, отпоран на многе групе антибиотика и доводи до појаве болничких инфекција. Светска здравствена организација прогласила је A. baumannii за патогенa који ефикасно избегава ефекат конвенционалних антибиотика, тако да је потреба за новим антимикробним средствима евидентна. Природни производи укључујући етарска уља су препозната као добри антимикробни агенси. Цимет је вековима познат зачин који има широку примену и за етарско уље (ЕУ цимета) је показано да поседује различите биолошке активности. Циљ ове студије је било испитивање ефекта ЕУ цимета и његове новосинтетисане наноемулзије (НА) на формирање биофилма A. baumannii. ЕУ цимета је хемијски окарактерисано применом GC/MS анализе. Величина капи и полидисперзитет припремљених НА испитани су фотонском корелационом спектроскопијом. МИК вредности су одређене микродилуционом методом, а антибиофилм активност бојењем кристал виолетом. GC/MS анализа је показала да је најзаступљенија компонента испитиваног ЕУ транс-цинамалдехид (61,9%), док НА показују мултимодалну расподелу величине честица. МИК вредности су се кретале у опсегу 0,25-0,5 mg/mL за EУ и 0,125-0,25 mg/mL за НА. Инхибиција формирања биофилма се кретала до 59% и 69%, за ЕУ и НА. Добијени резултати показују да НА има јачи антимикробни и антибиофилм ефекат, па се може рећи да би емулзификација могла бити приступ ка ефикаснијем сузбијању инфекција А. baumannii.
PB  - Београд : Српско биолошко друштво
C3  - Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.
T1  - Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii
SP  - 255
EP  - 255
UR  - https://hdl.handle.net/21.15107/rcub_dais_13611
ER  - 

@conference{
author = "Ганић, Теа and Вулетић, Стефана and Цветковић, Стефана and Стевановић, Магдалена and Кузмановић, Маја and Ђуровић, Саша and Николић, Биљана and Митић-Ћулафић, Драгана",
year = "2022",
abstract = "Антимикробна резистенција представља велики проблем за јавно здравље због ограничених терапијских могућности. Acinetobacter baumannii је опортунистички патоген, отпоран на многе групе антибиотика и доводи до појаве болничких инфекција. Светска здравствена организација прогласила је A. baumannii за патогенa који ефикасно избегава ефекат конвенционалних антибиотика, тако да је потреба за новим антимикробним средствима евидентна. Природни производи укључујући етарска уља су препозната као добри антимикробни агенси. Цимет је вековима познат зачин који има широку примену и за етарско уље (ЕУ цимета) је показано да поседује различите биолошке активности. Циљ ове студије је било испитивање ефекта ЕУ цимета и његове новосинтетисане наноемулзије (НА) на формирање биофилма A. baumannii. ЕУ цимета је хемијски окарактерисано применом GC/MS анализе. Величина капи и полидисперзитет припремљених НА испитани су фотонском корелационом спектроскопијом. МИК вредности су одређене микродилуционом методом, а антибиофилм активност бојењем кристал виолетом. GC/MS анализа је показала да је најзаступљенија компонента испитиваног ЕУ транс-цинамалдехид (61,9%), док НА показују мултимодалну расподелу величине честица. МИК вредности су се кретале у опсегу 0,25-0,5 mg/mL за EУ и 0,125-0,25 mg/mL за НА. Инхибиција формирања биофилма се кретала до 59% и 69%, за ЕУ и НА. Добијени резултати показују да НА има јачи антимикробни и антибиофилм ефекат, па се може рећи да би емулзификација могла бити приступ ка ефикаснијем сузбијању инфекција А. baumannii.",
publisher = "Београд : Српско биолошко друштво",
journal = "Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.",
title = "Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii",
pages = "255-255",
url = "https://hdl.handle.net/21.15107/rcub_dais_13611"
}

Ганић, Т., Вулетић, С., Цветковић, С., Стевановић, М., Кузмановић, М., Ђуровић, С., Николић, Б.,& Митић-Ћулафић, Д.. (2022). Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii. in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.
Београд : Српско биолошко друштво., 255-255.
https://hdl.handle.net/21.15107/rcub_dais_13611

Ганић Т, Вулетић С, Цветковић С, Стевановић М, Кузмановић М, Ђуровић С, Николић Б, Митић-Ћулафић Д. Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii. in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022.. 2022;:255-255.
https://hdl.handle.net/21.15107/rcub_dais_13611 .

Ганић, Теа, Вулетић, Стефана, Цветковић, Стефана, Стевановић, Магдалена, Кузмановић, Маја, Ђуровић, Саша, Николић, Биљана, Митић-Ћулафић, Драгана, "Ефекат етарског уља цимета и његове наноемулзије на формирање биофилма Acinetobacter baumannii" in Основна и примењена истраживања, методика наставе : књига сажетака / Трећи Конгрес биолога Србије, Златибор, Србија 21-25. 9. 2022. (2022):255-255,
https://hdl.handle.net/21.15107/rcub_dais_13611 .