TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Vranješ Đurić, Sanja
AU  - Mitić, Žarko
AU  - Janković, Drina
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/537
AB  - In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-d,l-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo.

The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d50 = 72 nm are coated with polymers. Radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. 125I-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies.

The biodistribution of 125I-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both 125I-HAp/Ch and 125I-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling
SP  - 439
EP  - 446
VL  - 43
DO  - 10.1016/j.msec.2014.07.046
UR  - https://hdl.handle.net/21.15107/rcub_dais_537
ER  - 

@article{
author = "Ignjatović, Nenad and Vranješ Đurić, Sanja and Mitić, Žarko and Janković, Drina and Uskoković, Dragan",
year = "2014",
abstract = "In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-d,l-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo.

The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d50 = 72 nm are coated with polymers. Radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. 125I-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies.

The biodistribution of 125I-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both 125I-HAp/Ch and 125I-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling",
pages = "439-446",
volume = "43",
doi = "10.1016/j.msec.2014.07.046",
url = "https://hdl.handle.net/21.15107/rcub_dais_537"
}

Ignjatović, N., Vranješ Đurić, S., Mitić, Ž., Janković, D.,& Uskoković, D.. (2014). Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling. in Materials Science and Engineering C
Elsevier., 43, 439-446.
https://doi.org/10.1016/j.msec.2014.07.046
https://hdl.handle.net/21.15107/rcub_dais_537

Ignjatović N, Vranješ Đurić S, Mitić Ž, Janković D, Uskoković D. Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling. in Materials Science and Engineering C. 2014;43:439-446.
doi:10.1016/j.msec.2014.07.046
https://hdl.handle.net/21.15107/rcub_dais_537 .

Ignjatović, Nenad, Vranješ Đurić, Sanja, Mitić, Žarko, Janković, Drina, Uskoković, Dragan, "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling" in Materials Science and Engineering C, 43 (2014):439-446,
https://doi.org/10.1016/j.msec.2014.07.046 .,
https://hdl.handle.net/21.15107/rcub_dais_537 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Vranješ Đurić, Sanja
AU  - Mitić, Žarko
AU  - Janković, Drina
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/539
AB  - In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-d,l-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo.

The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d50 = 72 nm are coated with polymers. Radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. 125I-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies.

The biodistribution of 125I-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both 125I-HAp/Ch and 125I-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs.
PB  - Elsevier
T2  - Materials Science and Engineering: C
T1  - Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling
SP  - 439
EP  - 436
VL  - 43
DO  - 10.1016/j.msec.2014.07.046
UR  - https://hdl.handle.net/21.15107/rcub_dais_539
ER  - 

@article{
author = "Ignjatović, Nenad and Vranješ Đurić, Sanja and Mitić, Žarko and Janković, Drina and Uskoković, Dragan",
year = "2014",
abstract = "In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-d,l-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo.

The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d50 = 72 nm are coated with polymers. Radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. 125I-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies.

The biodistribution of 125I-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both 125I-HAp/Ch and 125I-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs.",
publisher = "Elsevier",
journal = "Materials Science and Engineering: C",
title = "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling",
pages = "439-436",
volume = "43",
doi = "10.1016/j.msec.2014.07.046",
url = "https://hdl.handle.net/21.15107/rcub_dais_539"
}

Ignjatović, N., Vranješ Đurić, S., Mitić, Ž., Janković, D.,& Uskoković, D.. (2014). Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling. in Materials Science and Engineering: C
Elsevier., 43, 439-436.
https://doi.org/10.1016/j.msec.2014.07.046
https://hdl.handle.net/21.15107/rcub_dais_539

Ignjatović N, Vranješ Đurić S, Mitić Ž, Janković D, Uskoković D. Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling. in Materials Science and Engineering: C. 2014;43:439-436.
doi:10.1016/j.msec.2014.07.046
https://hdl.handle.net/21.15107/rcub_dais_539 .

Ignjatović, Nenad, Vranješ Đurić, Sanja, Mitić, Žarko, Janković, Drina, Uskoković, Dragan, "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive 125Iodine labeling" in Materials Science and Engineering: C, 43 (2014):439-436,
https://doi.org/10.1016/j.msec.2014.07.046 .,
https://hdl.handle.net/21.15107/rcub_dais_539 .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Vranješ Đurić, Sanja
AU  - Mitić, Žarko
AU  - Janković, Drina
AU  - Uskoković, Dragan
PY  - 2013
UR  - https://dais.sanu.ac.rs/123456789/393
AB  - Nanoparticles based on hydroxyapatite (NPs) have many useful physicochemical and biological
properties, such as easy preparation and modification, as well as biocompatibility, which make them suitable for transport and unloading of various pharmaceuticals. Hydroxyapatite
nanoparticles coated with bioresorbable polymers have been successfully used as carriers of
antibiotics and vitamins in bone tissue engineering. In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite and hydroxyapatite coated with chitosan and the chitosan-poly-D,L-lactide-co-glycolide polymer blend. The influence of the processing technique on the structure and characteristics of the obtained particles was studied by X-ray diffraction (XRD), particle size distribution analysis (PSD), Fourier transform infrared spectroscopy (FTIR), zeta potential analysis (ZP) and scanning electronic microscopy (SEM). In applied research, an appropriate radioisotope (Iodine-125) was selected and it was used to label particles. The in vivo biodistribution of 125I-labeled particles were studied in healthy Wistar rats following intravenous administration. The XRD, FT-IR and ZP analyses have confirmed that the hydroxyapatite particles with d50=72 nm are coated with chitosan and the chitosan-poly-D,L-lactide-co-glycolide polymer blend. 125I-labeled particles showed completely different behaviour in vivo: hydroxyapatite particles have the highest liver accumulation 10 min after injection but rapid excretion from the body without residual radioactivity 24 hours after injection; chitozan coated hydroxyapatite particles have the highest accumulation in the liver 10 min after injection with considerable amount (almost 50 %) retained 24 hours later; hydroxyapatite particles coated with the chitosanpoly-D,L-lactide-co-glycolide polymer blend have the highest uptake in the lungs 10 minutes after injection and moderate retention in the same organ 24 hours later.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
T1  - Routes and pathways to small particles based on hydroxyapatite
SP  - 7
EP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_dais_393
ER  - 

@conference{
author = "Ignjatović, Nenad and Vranješ Đurić, Sanja and Mitić, Žarko and Janković, Drina and Uskoković, Dragan",
year = "2013",
abstract = "Nanoparticles based on hydroxyapatite (NPs) have many useful physicochemical and biological
properties, such as easy preparation and modification, as well as biocompatibility, which make them suitable for transport and unloading of various pharmaceuticals. Hydroxyapatite
nanoparticles coated with bioresorbable polymers have been successfully used as carriers of
antibiotics and vitamins in bone tissue engineering. In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite and hydroxyapatite coated with chitosan and the chitosan-poly-D,L-lactide-co-glycolide polymer blend. The influence of the processing technique on the structure and characteristics of the obtained particles was studied by X-ray diffraction (XRD), particle size distribution analysis (PSD), Fourier transform infrared spectroscopy (FTIR), zeta potential analysis (ZP) and scanning electronic microscopy (SEM). In applied research, an appropriate radioisotope (Iodine-125) was selected and it was used to label particles. The in vivo biodistribution of 125I-labeled particles were studied in healthy Wistar rats following intravenous administration. The XRD, FT-IR and ZP analyses have confirmed that the hydroxyapatite particles with d50=72 nm are coated with chitosan and the chitosan-poly-D,L-lactide-co-glycolide polymer blend. 125I-labeled particles showed completely different behaviour in vivo: hydroxyapatite particles have the highest liver accumulation 10 min after injection but rapid excretion from the body without residual radioactivity 24 hours after injection; chitozan coated hydroxyapatite particles have the highest accumulation in the liver 10 min after injection with considerable amount (almost 50 %) retained 24 hours later; hydroxyapatite particles coated with the chitosanpoly-D,L-lactide-co-glycolide polymer blend have the highest uptake in the lungs 10 minutes after injection and moderate retention in the same organ 24 hours later.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts",
title = "Routes and pathways to small particles based on hydroxyapatite",
pages = "7-7",
url = "https://hdl.handle.net/21.15107/rcub_dais_393"
}

Ignjatović, N., Vranješ Đurić, S., Mitić, Ž., Janković, D.,& Uskoković, D.. (2013). Routes and pathways to small particles based on hydroxyapatite. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 7-7.
https://hdl.handle.net/21.15107/rcub_dais_393

Ignjatović N, Vranješ Đurić S, Mitić Ž, Janković D, Uskoković D. Routes and pathways to small particles based on hydroxyapatite. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:7-7.
https://hdl.handle.net/21.15107/rcub_dais_393 .

Ignjatović, Nenad, Vranješ Đurić, Sanja, Mitić, Žarko, Janković, Drina, Uskoković, Dragan, "Routes and pathways to small particles based on hydroxyapatite" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):7-7,
https://hdl.handle.net/21.15107/rcub_dais_393 .