TY  - CONF
AU  - Piljević, Miljana
AU  - Dinić, Ivana
AU  - Bukumira, Marta
AU  - Rabasović, Mihailo
AU  - Krmpot, Aleksandar
AU  - Lazarević, Miloš
AU  - Mančić, Lidija
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16596
AB  - Cancer represents one of the leading problems of today, with clinical detection oftentimes being difficult, currently based on imaging techniques, such as X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). However, mortality rate is often reduced by early detection, therefore much focus has been directed towards developing methods for early detection of the disease.
Recent research in the field of nanotechnology is focused on the use of nanoparticles, particularly Lanthanide-doped up-conversion nanoparticles (UCNPs), for the detection of cancer cells using near infrared (NIR) fluorescence microscopy. The reason for this is that in long-term tracking tests, nearinfrared
(NIR) light, has lower phototoxicity and higher tissue penetration depth in living systems as compared with UV/VIS light. In this research, NaGd0.8Yb0.17Er0.03F4 UCNPs were prepared by solvothermal synthesis in the presence of chitosan, a ligand which enables UCNPs biocompatibility and the specific antibody conjugation. Morphological and structural characterization of synthetized UCNPs were performed based on X-ray powder diffraction (XRPD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and
photoluminescence spectroscopy (PL). Results confirmed the presence of the cubic phase with a minor portion of hexagonal phase in nanoparticles. Synthesized nanoparticles were conjugated further with anti-human CD44 antibodies, labeled with fluorescein isothiocyanate (FITC), which signal is used for confirmation of nanoparticles positioning in cells. Such obtained conjugates were successfully used for selective in vitro biolabeling of oral squamous cell carcinoma cells.
PB  - Belgrade : Institute of Physics
C3  - Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
T1  - Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles
SP  - 38
EP  - 38
ER  - 

@conference{
author = "Piljević, Miljana and Dinić, Ivana and Bukumira, Marta and Rabasović, Mihailo and Krmpot, Aleksandar and Lazarević, Miloš and Mančić, Lidija",
year = "2024",
abstract = "Cancer represents one of the leading problems of today, with clinical detection oftentimes being difficult, currently based on imaging techniques, such as X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). However, mortality rate is often reduced by early detection, therefore much focus has been directed towards developing methods for early detection of the disease.
Recent research in the field of nanotechnology is focused on the use of nanoparticles, particularly Lanthanide-doped up-conversion nanoparticles (UCNPs), for the detection of cancer cells using near infrared (NIR) fluorescence microscopy. The reason for this is that in long-term tracking tests, nearinfrared
(NIR) light, has lower phototoxicity and higher tissue penetration depth in living systems as compared with UV/VIS light. In this research, NaGd0.8Yb0.17Er0.03F4 UCNPs were prepared by solvothermal synthesis in the presence of chitosan, a ligand which enables UCNPs biocompatibility and the specific antibody conjugation. Morphological and structural characterization of synthetized UCNPs were performed based on X-ray powder diffraction (XRPD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and
photoluminescence spectroscopy (PL). Results confirmed the presence of the cubic phase with a minor portion of hexagonal phase in nanoparticles. Synthesized nanoparticles were conjugated further with anti-human CD44 antibodies, labeled with fluorescein isothiocyanate (FITC), which signal is used for confirmation of nanoparticles positioning in cells. Such obtained conjugates were successfully used for selective in vitro biolabeling of oral squamous cell carcinoma cells.",
publisher = "Belgrade : Institute of Physics",
journal = "Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024",
title = "Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles",
pages = "38-38"
}

Piljević, M., Dinić, I., Bukumira, M., Rabasović, M., Krmpot, A., Lazarević, M.,& Mančić, L.. (2024). Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles. in Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
Belgrade : Institute of Physics., 38-38.

Piljević M, Dinić I, Bukumira M, Rabasović M, Krmpot A, Lazarević M, Mančić L. Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles. in Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024. 2024;:38-38..

Piljević, Miljana, Dinić, Ivana, Bukumira, Marta, Rabasović, Mihailo, Krmpot, Aleksandar, Lazarević, Miloš, Mančić, Lidija, "Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles" in Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024 (2024):38-38.

TY  - JOUR
AU  - Dinić, Ivana
AU  - Vuković, Marina
AU  - Rabanal, Maria Eugenia
AU  - Milošević, Milica
AU  - Bukumira, Marta
AU  - Tomić, Nina
AU  - Tomić, Miloš
AU  - Mančić, Lidija
AU  - Ignjatović, Nenad
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16211
AB  - Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation.
PB  - Basel : MDPI AG
T2  - Journal of Functional Biomaterials
T1  - Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals
SP  - 6
VL  - 15
IS  - 1
DO  - 10.3390/jfb15010006
UR  - https://hdl.handle.net/21.15107/rcub_dais_16211
ER  - 

@article{
author = "Dinić, Ivana and Vuković, Marina and Rabanal, Maria Eugenia and Milošević, Milica and Bukumira, Marta and Tomić, Nina and Tomić, Miloš and Mančić, Lidija and Ignjatović, Nenad",
year = "2024",
abstract = "Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation.",
publisher = "Basel : MDPI AG",
journal = "Journal of Functional Biomaterials",
title = "Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals",
pages = "6",
volume = "15",
number = "1",
doi = "10.3390/jfb15010006",
url = "https://hdl.handle.net/21.15107/rcub_dais_16211"
}

Dinić, I., Vuković, M., Rabanal, M. E., Milošević, M., Bukumira, M., Tomić, N., Tomić, M., Mančić, L.,& Ignjatović, N.. (2024). Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals. in Journal of Functional Biomaterials
Basel : MDPI AG., 15(1), 6.
https://doi.org/10.3390/jfb15010006
https://hdl.handle.net/21.15107/rcub_dais_16211

Dinić I, Vuković M, Rabanal ME, Milošević M, Bukumira M, Tomić N, Tomić M, Mančić L, Ignjatović N. Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals. in Journal of Functional Biomaterials. 2024;15(1):6.
doi:10.3390/jfb15010006
https://hdl.handle.net/21.15107/rcub_dais_16211 .

Dinić, Ivana, Vuković, Marina, Rabanal, Maria Eugenia, Milošević, Milica, Bukumira, Marta, Tomić, Nina, Tomić, Miloš, Mančić, Lidija, Ignjatović, Nenad, "Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals" in Journal of Functional Biomaterials, 15, no. 1 (2024):6,
https://doi.org/10.3390/jfb15010006 .,
https://hdl.handle.net/21.15107/rcub_dais_16211 .

TY  - CONF
AU  - Tomić, Nina
AU  - Dinić, Ivana
AU  - Vuković, Marina
AU  - Bukumira, Marta
AU  - Rabasović, Mihailo
AU  - Mančić, Lidija
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15155
AB  - There is a great technological interest in synthesis of lanthanide doped upconverting nanoparticles (UCNPs) with controlled crystal phase, morphology and intense luminescence properties suitable for biomedical use. A conventional approach for synthesis of such particles comprises decomposition of organometallic compounds in an oxygen-free environment, followed either with a ligand exchange, or biocompatible layer coating. Biocompatible NaY0.65Gd0.15F4:Yb,Er nanoparticles used in this study were synthesized through chitosan assisted one-pot hydrothermal synthesis and were characterized by X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDS) and photoluminescence measurement (PL). Due to the presence of the amino groups at their surface, excellent biocompatibility and notably low cytotoxicity against MRC-5 cells (line of normal human fibroblasts) and A549 cells (human lung cancer cells) were detected using MTT assay. Furthermore, upon 980 nm laser irradiation, particles were successfully used in vitro for labeling of both, MRC-5 and A549 cells.
PB  - Belgrade : Serbian Ceramic Society
C3  - Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023
T1  - One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_dais_15155
ER  - 

@conference{
author = "Tomić, Nina and Dinić, Ivana and Vuković, Marina and Bukumira, Marta and Rabasović, Mihailo and Mančić, Lidija",
year = "2023",
abstract = "There is a great technological interest in synthesis of lanthanide doped upconverting nanoparticles (UCNPs) with controlled crystal phase, morphology and intense luminescence properties suitable for biomedical use. A conventional approach for synthesis of such particles comprises decomposition of organometallic compounds in an oxygen-free environment, followed either with a ligand exchange, or biocompatible layer coating. Biocompatible NaY0.65Gd0.15F4:Yb,Er nanoparticles used in this study were synthesized through chitosan assisted one-pot hydrothermal synthesis and were characterized by X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDS) and photoluminescence measurement (PL). Due to the presence of the amino groups at their surface, excellent biocompatibility and notably low cytotoxicity against MRC-5 cells (line of normal human fibroblasts) and A549 cells (human lung cancer cells) were detected using MTT assay. Furthermore, upon 980 nm laser irradiation, particles were successfully used in vitro for labeling of both, MRC-5 and A549 cells.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023",
title = "One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_dais_15155"
}

Tomić, N., Dinić, I., Vuković, M., Bukumira, M., Rabasović, M.,& Mančić, L.. (2023). One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023
Belgrade : Serbian Ceramic Society., 70-70.
https://hdl.handle.net/21.15107/rcub_dais_15155

Tomić N, Dinić I, Vuković M, Bukumira M, Rabasović M, Mančić L. One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023. 2023;:70-70.
https://hdl.handle.net/21.15107/rcub_dais_15155 .

Tomić, Nina, Dinić, Ivana, Vuković, Marina, Bukumira, Marta, Rabasović, Mihailo, Mančić, Lidija, "One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging" in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023 (2023):70-70,
https://hdl.handle.net/21.15107/rcub_dais_15155 .