TY  - JOUR
AU  - Jakovetić, Sonja
AU  - Luković, Nevena
AU  - Jugović, Branimir
AU  - Gvozdenović, Milica M.
AU  - Grbavčić, Sanja
AU  - Jovanović, Jelena
AU  - Knežević Jugović, Zorica
PY  - 2015
UR  - https://dais.sanu.ac.rs/123456789/3528
AB  - The objective of this research was to design an efficient continuously operated membrane reactor with a separation unit for egg white protein (EWP) hydrolysis and production of hydrolysates with improved antioxidant properties. For this purpose, a mechanically stirred tank reactor coupled with the polyethersulfone ultrafiltration module with a molecular weight cutoff of 10 kDa was employed. Several proteolytic enzymes have been tested in order to obtain the best quality of peptide-based formulations intended for human consumption. Among protease from Bacillus licheniformis (Alcalase), protease from Bacillus amyloliquefaciens (Neutrase), and protease from papaya latex (papain), the highest degree of hydrolysis (DH), as well as the best antioxidant properties of obtained hydrolysates, was achieved with Alcalase. The effects of operating variables such as enzyme/substrate ([E]/[S]) ratio, impeller speed, and permeate flow rate were further studied using response surface methodology (RSM) and Box–Behnken experimental design. Results obtained in RSM analysis confirmed that over the studied range [E]/[S] ratio, impeller speed and permeate flow rate had the significant effect on the DH and reactor capacity. The effects of different impeller geometries were also studied and four-bladed propeller stirrer enabled the highest DH. Antioxidant properties were analyzed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), by the 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, and by the linear voltammetry methods. Results show that the use of Alcalase in the membrane reactor system is of potential interest for the EWP hydrolysis and obtaining value-added egg products. © 2014, Springer Science+Business Media New York.
PB  - Springer
T2  - Food and Bioprocess Technology
T1  - Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit
SP  - 287
EP  - 300
VL  - 8
IS  - 2
DO  - 10.1007/s11947-014-1402-y
UR  - https://hdl.handle.net/21.15107/rcub_dais_3528
ER  - 

@article{
author = "Jakovetić, Sonja and Luković, Nevena and Jugović, Branimir and Gvozdenović, Milica M. and Grbavčić, Sanja and Jovanović, Jelena and Knežević Jugović, Zorica",
year = "2015",
abstract = "The objective of this research was to design an efficient continuously operated membrane reactor with a separation unit for egg white protein (EWP) hydrolysis and production of hydrolysates with improved antioxidant properties. For this purpose, a mechanically stirred tank reactor coupled with the polyethersulfone ultrafiltration module with a molecular weight cutoff of 10 kDa was employed. Several proteolytic enzymes have been tested in order to obtain the best quality of peptide-based formulations intended for human consumption. Among protease from Bacillus licheniformis (Alcalase), protease from Bacillus amyloliquefaciens (Neutrase), and protease from papaya latex (papain), the highest degree of hydrolysis (DH), as well as the best antioxidant properties of obtained hydrolysates, was achieved with Alcalase. The effects of operating variables such as enzyme/substrate ([E]/[S]) ratio, impeller speed, and permeate flow rate were further studied using response surface methodology (RSM) and Box–Behnken experimental design. Results obtained in RSM analysis confirmed that over the studied range [E]/[S] ratio, impeller speed and permeate flow rate had the significant effect on the DH and reactor capacity. The effects of different impeller geometries were also studied and four-bladed propeller stirrer enabled the highest DH. Antioxidant properties were analyzed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), by the 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, and by the linear voltammetry methods. Results show that the use of Alcalase in the membrane reactor system is of potential interest for the EWP hydrolysis and obtaining value-added egg products. © 2014, Springer Science+Business Media New York.",
publisher = "Springer",
journal = "Food and Bioprocess Technology",
title = "Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit",
pages = "287-300",
volume = "8",
number = "2",
doi = "10.1007/s11947-014-1402-y",
url = "https://hdl.handle.net/21.15107/rcub_dais_3528"
}

Jakovetić, S., Luković, N., Jugović, B., Gvozdenović, M. M., Grbavčić, S., Jovanović, J.,& Knežević Jugović, Z.. (2015). Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit. in Food and Bioprocess Technology
Springer., 8(2), 287-300.
https://doi.org/10.1007/s11947-014-1402-y
https://hdl.handle.net/21.15107/rcub_dais_3528

Jakovetić S, Luković N, Jugović B, Gvozdenović MM, Grbavčić S, Jovanović J, Knežević Jugović Z. Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit. in Food and Bioprocess Technology. 2015;8(2):287-300.
doi:10.1007/s11947-014-1402-y
https://hdl.handle.net/21.15107/rcub_dais_3528 .

Jakovetić, Sonja, Luković, Nevena, Jugović, Branimir, Gvozdenović, Milica M., Grbavčić, Sanja, Jovanović, Jelena, Knežević Jugović, Zorica, "Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit" in Food and Bioprocess Technology, 8, no. 2 (2015):287-300,
https://doi.org/10.1007/s11947-014-1402-y .,
https://hdl.handle.net/21.15107/rcub_dais_3528 .

TY  - CONF
AU  - Jovanović, Jelena
AU  - Adnađević, Borivoj
AU  - Suljovrujić, Edin H.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2003
UR  - https://dais.sanu.ac.rs/123456789/2746
AB  - Injectable and gas-permeable composite cements, polydimethylsiloxane/hydroxyapatite (PDMS/HAp), were synthesized using bi-component polysiloxane precursors (RTV-A and RTV-B), which react by hydrosilylation, and different mass fractions of hydroxyapatite (HAp) having particle size of about 100 nm. The effect of HAp mass fraction (0-60%) on the thermodynamic and kinetic parameters of polysiloxanes cross-linking, i.e., PDMS/HAp composite preparation, was investigated by means of differential scanning calorimetry (DSC). It was determined that small mass fractions of HAp (up to about 10%) cause a decrease in temperature (T-max), and activation energy (E-a) of the investigated cross-linking process, while the total heat of the process (H-tot) increases. For the HAp mass fraction greater than 20%, an opposite effect was observed. The temperature and the time interval of cross-linking increase rapidly for smaller, but slowly, almost insignificantly, for larger HAp mass fractions. The determined order (n) of the reaction shows a trend similar to H-tot, but due to small variations found, this does not merit discussion.
C3  - Contemporary Studies in Advanced Materials and Processes, 4th Yugoslav-Materials-Research-Society Conference, Sep 10-14, 2001, Herceg Novi, Yugoslavia
T1  - Influence of the HAp ratio on the thermodynamic and kinetic parameters of PDMS/HAp composites cross-linking
SP  - 263
EP  - 268
DO  - 10.4028/www.scientific.net/MSF.413.263
UR  - https://hdl.handle.net/21.15107/rcub_dais_2746
ER  - 

@conference{
author = "Jovanović, Jelena and Adnađević, Borivoj and Suljovrujić, Edin H. and Ignjatović, Nenad and Uskoković, Dragan",
year = "2003",
abstract = "Injectable and gas-permeable composite cements, polydimethylsiloxane/hydroxyapatite (PDMS/HAp), were synthesized using bi-component polysiloxane precursors (RTV-A and RTV-B), which react by hydrosilylation, and different mass fractions of hydroxyapatite (HAp) having particle size of about 100 nm. The effect of HAp mass fraction (0-60%) on the thermodynamic and kinetic parameters of polysiloxanes cross-linking, i.e., PDMS/HAp composite preparation, was investigated by means of differential scanning calorimetry (DSC). It was determined that small mass fractions of HAp (up to about 10%) cause a decrease in temperature (T-max), and activation energy (E-a) of the investigated cross-linking process, while the total heat of the process (H-tot) increases. For the HAp mass fraction greater than 20%, an opposite effect was observed. The temperature and the time interval of cross-linking increase rapidly for smaller, but slowly, almost insignificantly, for larger HAp mass fractions. The determined order (n) of the reaction shows a trend similar to H-tot, but due to small variations found, this does not merit discussion.",
journal = "Contemporary Studies in Advanced Materials and Processes, 4th Yugoslav-Materials-Research-Society Conference, Sep 10-14, 2001, Herceg Novi, Yugoslavia",
title = "Influence of the HAp ratio on the thermodynamic and kinetic parameters of PDMS/HAp composites cross-linking",
pages = "263-268",
doi = "10.4028/www.scientific.net/MSF.413.263",
url = "https://hdl.handle.net/21.15107/rcub_dais_2746"
}

Jovanović, J., Adnađević, B., Suljovrujić, E. H., Ignjatović, N.,& Uskoković, D.. (2003). Influence of the HAp ratio on the thermodynamic and kinetic parameters of PDMS/HAp composites cross-linking. in Contemporary Studies in Advanced Materials and Processes, 4th Yugoslav-Materials-Research-Society Conference, Sep 10-14, 2001, Herceg Novi, Yugoslavia, 263-268.
https://doi.org/10.4028/www.scientific.net/MSF.413.263
https://hdl.handle.net/21.15107/rcub_dais_2746

Jovanović J, Adnađević B, Suljovrujić EH, Ignjatović N, Uskoković D. Influence of the HAp ratio on the thermodynamic and kinetic parameters of PDMS/HAp composites cross-linking. in Contemporary Studies in Advanced Materials and Processes, 4th Yugoslav-Materials-Research-Society Conference, Sep 10-14, 2001, Herceg Novi, Yugoslavia. 2003;:263-268.
doi:10.4028/www.scientific.net/MSF.413.263
https://hdl.handle.net/21.15107/rcub_dais_2746 .

Jovanović, Jelena, Adnađević, Borivoj, Suljovrujić, Edin H., Ignjatović, Nenad, Uskoković, Dragan, "Influence of the HAp ratio on the thermodynamic and kinetic parameters of PDMS/HAp composites cross-linking" in Contemporary Studies in Advanced Materials and Processes, 4th Yugoslav-Materials-Research-Society Conference, Sep 10-14, 2001, Herceg Novi, Yugoslavia (2003):263-268,
https://doi.org/10.4028/www.scientific.net/MSF.413.263 .,
https://hdl.handle.net/21.15107/rcub_dais_2746 .