Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles
Само за регистроване кориснике
2022
Аутори
Stevanović, MagdalenaFilipović, Nenad
Kuzmanović, Maja
Tomić, Nina
Ušjak, Dušan
Milenković, Marina
Zheng, Kai
Stampfl, Juergen
Boccaccini, Aldo
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It ...was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022.
Кључне речи:
scanning electron microscopy (SEM) / biocompatibility / biomaterials / composite materials / scaffolds / antibacterial agents / antimicrobial activity / bacteria / biomaterials / composite materials / differential scanning calorimetry / escherichia coli / extracellular matrices / Fourier transform infrared spectroscopy / MTT assay / nanoparticles / pseudomonas aeruginosa / scaffolds / staphylococcus aureus / synthesis (chemical) / X ray diffraction / antibacterial activity / antimicrobial activity / biofilm / biomaterial / bone tissue / calcium phosphate / cell viability / collagen gel / colorimetry / cytotoxicity / dimethyl sulfoxide / extracellular matrix / field emission scanning electron microscopy / human cells / quantitative analysis / scaffold protein / stereolithography / tissue engineering / zeta potential / selenium nanoparticles / biofilms / bacillus subtilis / Field emission microscopy / thermal analysis / 3D printers / differential scanning calorimetry / light scattering / composite materials / health risks / bone tissue engineering / Candida albicans / Klebsiella pneumoniae / bone metabolism / Enterococcus faecalis / inductively coupled plasma mass spectrometry / mesenchymal stem cell / MRC-5 cell line / multidrug resistants / photon correlation spectroscopy / Salmonella enterica serovar Typhimurium / sodium selenite / triphenyltetrazoliumИзвор:
Journal of Biomaterials Applications, 2022, 36, 10, 1800-1811Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200175 (Институт техничких наука САНУ, Београд) (RS-MESTD-inst-2020-200175)
- Bilateral project between Serbia and Austria (project No: SRB 24/2018, project title: Scaffolds with controlled 3-D architecture designed by photopolymerization)
- Bilateral project between Serbia and Germany (DAAD project 57514776)
DOI: 10.1177/08853282211073731
ISSN: 0885-3282
WoS: 000764244600001
Scopus: 2-s2.0-85126034982
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Stevanović, Magdalena AU - Filipović, Nenad AU - Kuzmanović, Maja AU - Tomić, Nina AU - Ušjak, Dušan AU - Milenković, Marina AU - Zheng, Kai AU - Stampfl, Juergen AU - Boccaccini, Aldo PY - 2022 UR - https://dais.sanu.ac.rs/123456789/13579 AB - Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022. T2 - Journal of Biomaterials Applications T1 - Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles SP - 1800 EP - 1811 VL - 36 IS - 10 DO - 10.1177/08853282211073731 UR - https://hdl.handle.net/21.15107/rcub_dais_13579 ER -
@article{ author = "Stevanović, Magdalena and Filipović, Nenad and Kuzmanović, Maja and Tomić, Nina and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Stampfl, Juergen and Boccaccini, Aldo", year = "2022", abstract = "Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones. © The Author(s) 2022.", journal = "Journal of Biomaterials Applications", title = "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles", pages = "1800-1811", volume = "36", number = "10", doi = "10.1177/08853282211073731", url = "https://hdl.handle.net/21.15107/rcub_dais_13579" }
Stevanović, M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J.,& Boccaccini, A.. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications, 36(10), 1800-1811. https://doi.org/10.1177/08853282211073731 https://hdl.handle.net/21.15107/rcub_dais_13579
Stevanović M, Filipović N, Kuzmanović M, Tomić N, Ušjak D, Milenković M, Zheng K, Stampfl J, Boccaccini A. Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications. 2022;36(10):1800-1811. doi:10.1177/08853282211073731 https://hdl.handle.net/21.15107/rcub_dais_13579 .
Stevanović, Magdalena, Filipović, Nenad, Kuzmanović, Maja, Tomić, Nina, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Stampfl, Juergen, Boccaccini, Aldo, "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles" in Journal of Biomaterials Applications, 36, no. 10 (2022):1800-1811, https://doi.org/10.1177/08853282211073731 ., https://hdl.handle.net/21.15107/rcub_dais_13579 .