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Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
dc.creator | Stupar, Petar | |
dc.creator | Pavlović, Vladimir B. | |
dc.creator | Nunić, Jana | |
dc.creator | Cundrič, Sandra | |
dc.creator | Filipič, Metka | |
dc.creator | Stevanović, Magdalena | |
dc.date.accessioned | 2017-06-10T15:45:07Z | |
dc.date.accessioned | 2018-12-23T08:45:50Z | |
dc.date.available | 2018-12-23T08:45:50Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1773-2247 | |
dc.identifier.uri | https://dais.sanu.ac.rs/123456789/4671 | |
dc.description.abstract | A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes. | en |
dc.format | application/pdf | |
dc.language | en | |
dc.publisher | Paris : Association de pharmacie galenique industrielle | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Drug Delivery Science and Technology | en |
dc.subject | poly(epsilon- caprolactone) | |
dc.subject | reactive oxygen species | |
dc.subject | freeze drying | |
dc.subject | physicochemical solvent and non-solvent approach | |
dc.title | Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Стевановић, Магдалена; Ступар, Петар; Павловић, Владимир Б.; Нунић, Јана; Цундрич, Сандра; Филипич, Метка; | |
dc.citation.spage | 191 | |
dc.citation.epage | 197 | |
dc.citation.volume | 24 | |
dc.citation.issue | 2 | |
dc.identifier.wos | 000339928800011 | |
dc.identifier.doi | 10.1016/S1773-2247(14)50031-7 | |
dc.identifier.scopus | 2-s2.0-84899567719 | |
dc.type.version | publishedVersion | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_dais_4671 |