Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery
Аутори
Kostić, Ivana T.Ilić, Vesna Lj.
Bukara, Katarina M.
Mojsilović, Slavko B.
Đurić, Zorka Ž.
Draškovič, Petra
Bugarski, Branko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bov...ine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected. © 2015, Association of Chemists and Chemical Engineers of Serbia. All rights reserved.
Кључне речи:
erythrocytes / flow cytometry / mechanical fragility / microcytic anaemia / osmotic fragility / osmotic swellingИзвор:
Hemijska industrija, 2015, 69, 1, 67-76Издавач:
- Belgrade: Association of the Chemical Engineers of Serbia
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Bilateral cooperation program between the Republic of Serbia and the Republic of Slovenia, 2012–2013
DOI: 10.2298/HEMIND140124021K
ISSN: 0367-598X (Print); 2217-7426
WoS: 000350913100009
Scopus: 2-s2.0-84923652588
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Kostić, Ivana T. AU - Ilić, Vesna Lj. AU - Bukara, Katarina M. AU - Mojsilović, Slavko B. AU - Đurić, Zorka Ž. AU - Draškovič, Petra AU - Bugarski, Branko PY - 2015 UR - https://dais.sanu.ac.rs/123456789/3533 AB - Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected. © 2015, Association of Chemists and Chemical Engineers of Serbia. All rights reserved. PB - Belgrade: Association of the Chemical Engineers of Serbia T2 - Hemijska industrija T1 - Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery SP - 67 EP - 76 VL - 69 IS - 1 DO - 10.2298/HEMIND140124021K UR - https://hdl.handle.net/21.15107/rcub_dais_3533 ER -
@article{ author = "Kostić, Ivana T. and Ilić, Vesna Lj. and Bukara, Katarina M. and Mojsilović, Slavko B. and Đurić, Zorka Ž. and Draškovič, Petra and Bugarski, Branko", year = "2015", abstract = "Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected. © 2015, Association of Chemists and Chemical Engineers of Serbia. All rights reserved.", publisher = "Belgrade: Association of the Chemical Engineers of Serbia", journal = "Hemijska industrija", title = "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery", pages = "67-76", volume = "69", number = "1", doi = "10.2298/HEMIND140124021K", url = "https://hdl.handle.net/21.15107/rcub_dais_3533" }
Kostić, I. T., Ilić, V. Lj., Bukara, K. M., Mojsilović, S. B., Đurić, Z. Ž., Draškovič, P.,& Bugarski, B.. (2015). Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija Belgrade: Association of the Chemical Engineers of Serbia., 69(1), 67-76. https://doi.org/10.2298/HEMIND140124021K https://hdl.handle.net/21.15107/rcub_dais_3533
Kostić IT, Ilić VL, Bukara KM, Mojsilović SB, Đurić ZŽ, Draškovič P, Bugarski B. Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija. 2015;69(1):67-76. doi:10.2298/HEMIND140124021K https://hdl.handle.net/21.15107/rcub_dais_3533 .
Kostić, Ivana T., Ilić, Vesna Lj., Bukara, Katarina M., Mojsilović, Slavko B., Đurić, Zorka Ž., Draškovič, Petra, Bugarski, Branko, "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery" in Hemijska industrija, 69, no. 1 (2015):67-76, https://doi.org/10.2298/HEMIND140124021K ., https://hdl.handle.net/21.15107/rcub_dais_3533 .