Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors
Само за регистроване кориснике
2013
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
We analyze the fluctuations of the equilibrium adsorbed mass in affinity-based biosensors, considering adsorption–desorption and mass transfer processes of two molecular species which compete for binding to the same probe molecules immobilized on the sensing surface. The analytical expression for the fluctuations spectral density is derived. The calculations show the significant influence of both the competitive adsorption and the mass transfer on the fluctuations spectrum, and also of their combined effect. The dependences of the fluctuations on association and dissociation rate constants of both adsorbing species, their concentrations, molecular masses and mass transfer coefficients and also on the density of capturing probes can be examined by using the presented model. The model is applicable for biosensors in which the spatial distribution of adsorbates concentrations in the reaction chamber can be approximated using the two-compartment model. The theory provides a more realistic ...estimation of the biosensors’ limiting performance, and is useful for their improvement.
Кључне речи:
biosensors / competitive binding / adsorption–desorption / mass transfer / fluctuationsИзвор:
Sensors and Actuators B: Chemical, 2013, 189, 71-79Издавач:
- Elsevier
Финансирање / пројекти:
- Микро, нано-системи и сензори за примену у електропривреди, процесној индустрији и заштити животне средине (RS-MESTD-Technological Development (TD or TR)-32008)
- Serbian Academy of Sciences and Arts, Project F-150
DOI: 10.1016/j.snb.2012.12.080
ISSN: 0925-4005
WoS: 000326346300011
Scopus: 2-s2.0-84887061320
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Frantlović, Miloš AU - Jokić, Ivana AU - Đurić, Zoran G. AU - Radulović, Katarina PY - 2013 UR - https://dais.sanu.ac.rs/123456789/350 AB - We analyze the fluctuations of the equilibrium adsorbed mass in affinity-based biosensors, considering adsorption–desorption and mass transfer processes of two molecular species which compete for binding to the same probe molecules immobilized on the sensing surface. The analytical expression for the fluctuations spectral density is derived. The calculations show the significant influence of both the competitive adsorption and the mass transfer on the fluctuations spectrum, and also of their combined effect. The dependences of the fluctuations on association and dissociation rate constants of both adsorbing species, their concentrations, molecular masses and mass transfer coefficients and also on the density of capturing probes can be examined by using the presented model. The model is applicable for biosensors in which the spatial distribution of adsorbates concentrations in the reaction chamber can be approximated using the two-compartment model. The theory provides a more realistic estimation of the biosensors’ limiting performance, and is useful for their improvement. PB - Elsevier T2 - Sensors and Actuators B: Chemical T1 - Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors SP - 71 EP - 79 VL - 189 DO - 10.1016/j.snb.2012.12.080 UR - https://hdl.handle.net/21.15107/rcub_dais_350 ER -
@article{ author = "Frantlović, Miloš and Jokić, Ivana and Đurić, Zoran G. and Radulović, Katarina", year = "2013", abstract = "We analyze the fluctuations of the equilibrium adsorbed mass in affinity-based biosensors, considering adsorption–desorption and mass transfer processes of two molecular species which compete for binding to the same probe molecules immobilized on the sensing surface. The analytical expression for the fluctuations spectral density is derived. The calculations show the significant influence of both the competitive adsorption and the mass transfer on the fluctuations spectrum, and also of their combined effect. The dependences of the fluctuations on association and dissociation rate constants of both adsorbing species, their concentrations, molecular masses and mass transfer coefficients and also on the density of capturing probes can be examined by using the presented model. The model is applicable for biosensors in which the spatial distribution of adsorbates concentrations in the reaction chamber can be approximated using the two-compartment model. The theory provides a more realistic estimation of the biosensors’ limiting performance, and is useful for their improvement.", publisher = "Elsevier", journal = "Sensors and Actuators B: Chemical", title = "Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors", pages = "71-79", volume = "189", doi = "10.1016/j.snb.2012.12.080", url = "https://hdl.handle.net/21.15107/rcub_dais_350" }
Frantlović, M., Jokić, I., Đurić, Z. G.,& Radulović, K.. (2013). Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors. in Sensors and Actuators B: Chemical Elsevier., 189, 71-79. https://doi.org/10.1016/j.snb.2012.12.080 https://hdl.handle.net/21.15107/rcub_dais_350
Frantlović M, Jokić I, Đurić ZG, Radulović K. Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors. in Sensors and Actuators B: Chemical. 2013;189:71-79. doi:10.1016/j.snb.2012.12.080 https://hdl.handle.net/21.15107/rcub_dais_350 .
Frantlović, Miloš, Jokić, Ivana, Đurić, Zoran G., Radulović, Katarina, "Analysis of the competitive adsorption and mass transfer influence on equilibrium mass fluctuations in affinity-based biosensors" in Sensors and Actuators B: Chemical, 189 (2013):71-79, https://doi.org/10.1016/j.snb.2012.12.080 ., https://hdl.handle.net/21.15107/rcub_dais_350 .