Size effect of calcium phosphate coated with poly-DL-lactideco-glycolide on healing processes in bone reconstruction
Abstract
In this article, synthesis and application of calcium phosphate/poly-DL- lactide-co-glycolide (CP/PLGA) composite biomaterial in particulate form, in which each CP granule/particle is coated with PLGA, are described. Two types of the particulate material having different particle sizes were synthesized: one with an average particle diameter between 150 and 250 μm (micron-sized particles, MPs) and the other with an average particle diameter smaller than 50 nm (nanoparticles, NPs). A comparative in vivo analysis was done by reconstructing defects in osteoporotic alveolar bones using both composites. The material, CP granules/particles covered with polymer, was characterized using X-ray structural analysis, scanning electron microscopy, and atomic force microscopy. Changes in reparatory functions of tissues affected by osteoporosis were examined in mice in vivo, using these two kinds of composite materials, with and without autologous plasma. Having defined the target segment, histomorpho...metric parameters - bone area fraction, area, and mean density - were determined. The best results in the regeneration and recuperation of alveolar bone damaged by osteoporosis were achieved with the implantation of a mixture of nanoparticulate CP/PLGA composite and autologous plasma. After the implantation of microparticulate CP/PLGA, in the form of granules, mixed with autologous plasma, into an artificial defect in alveolar bone, new bone formation was also observed, although its formation rate was slower. © 2010 Wiley Periodicals, Inc.
Keywords:
nanoparticles / microparticles / alveolar bone / autologous plasma / Calcium phosphate/poly-DL-lactide-co-glycolide (CP/PLGA)Source:
Journal of Biomedical Materials Research - Part B Applied Biomaterials, 2010, 94, 1, 108-117Publisher:
- Hoboken, NJ : John Wiley & Sons
Funding / projects:
- Sinteza funkcionalnih materijala sa kontrolisanom strukturom na molekularnom i nano nivou (RS-142006)
- Biokompatibilnost i mogućnosti primene biomaterijala na bazi hidroksiapatita i biopolimera u tkivnoj reparaciji - eksperimentalna i klinička studija (RS-145068)
DOI: 10.1002/jbm.b.31630
ISSN: 1552-4973
WoS: 000278697400012
Scopus: 2-s2.0-77954136401
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Ignjatović, Nenad AU - Ajduković, Zorica AU - Savić, Vojin. P. AU - Uskoković, Dragan PY - 2010 UR - https://dais.sanu.ac.rs/123456789/3419 AB - In this article, synthesis and application of calcium phosphate/poly-DL- lactide-co-glycolide (CP/PLGA) composite biomaterial in particulate form, in which each CP granule/particle is coated with PLGA, are described. Two types of the particulate material having different particle sizes were synthesized: one with an average particle diameter between 150 and 250 μm (micron-sized particles, MPs) and the other with an average particle diameter smaller than 50 nm (nanoparticles, NPs). A comparative in vivo analysis was done by reconstructing defects in osteoporotic alveolar bones using both composites. The material, CP granules/particles covered with polymer, was characterized using X-ray structural analysis, scanning electron microscopy, and atomic force microscopy. Changes in reparatory functions of tissues affected by osteoporosis were examined in mice in vivo, using these two kinds of composite materials, with and without autologous plasma. Having defined the target segment, histomorphometric parameters - bone area fraction, area, and mean density - were determined. The best results in the regeneration and recuperation of alveolar bone damaged by osteoporosis were achieved with the implantation of a mixture of nanoparticulate CP/PLGA composite and autologous plasma. After the implantation of microparticulate CP/PLGA, in the form of granules, mixed with autologous plasma, into an artificial defect in alveolar bone, new bone formation was also observed, although its formation rate was slower. © 2010 Wiley Periodicals, Inc. PB - Hoboken, NJ : John Wiley & Sons T2 - Journal of Biomedical Materials Research - Part B Applied Biomaterials T1 - Size effect of calcium phosphate coated with poly-DL-lactideco-glycolide on healing processes in bone reconstruction SP - 108 EP - 117 VL - 94 IS - 1 DO - 10.1002/jbm.b.31630 UR - https://hdl.handle.net/21.15107/rcub_dais_3419 ER -
@article{ author = "Ignjatović, Nenad and Ajduković, Zorica and Savić, Vojin. P. and Uskoković, Dragan", year = "2010", abstract = "In this article, synthesis and application of calcium phosphate/poly-DL- lactide-co-glycolide (CP/PLGA) composite biomaterial in particulate form, in which each CP granule/particle is coated with PLGA, are described. Two types of the particulate material having different particle sizes were synthesized: one with an average particle diameter between 150 and 250 μm (micron-sized particles, MPs) and the other with an average particle diameter smaller than 50 nm (nanoparticles, NPs). A comparative in vivo analysis was done by reconstructing defects in osteoporotic alveolar bones using both composites. The material, CP granules/particles covered with polymer, was characterized using X-ray structural analysis, scanning electron microscopy, and atomic force microscopy. Changes in reparatory functions of tissues affected by osteoporosis were examined in mice in vivo, using these two kinds of composite materials, with and without autologous plasma. Having defined the target segment, histomorphometric parameters - bone area fraction, area, and mean density - were determined. The best results in the regeneration and recuperation of alveolar bone damaged by osteoporosis were achieved with the implantation of a mixture of nanoparticulate CP/PLGA composite and autologous plasma. After the implantation of microparticulate CP/PLGA, in the form of granules, mixed with autologous plasma, into an artificial defect in alveolar bone, new bone formation was also observed, although its formation rate was slower. © 2010 Wiley Periodicals, Inc.", publisher = "Hoboken, NJ : John Wiley & Sons", journal = "Journal of Biomedical Materials Research - Part B Applied Biomaterials", title = "Size effect of calcium phosphate coated with poly-DL-lactideco-glycolide on healing processes in bone reconstruction", pages = "108-117", volume = "94", number = "1", doi = "10.1002/jbm.b.31630", url = "https://hdl.handle.net/21.15107/rcub_dais_3419" }
Ignjatović, N., Ajduković, Z., Savić, Vojin. P.,& Uskoković, D.. (2010). Size effect of calcium phosphate coated with poly-DL-lactideco-glycolide on healing processes in bone reconstruction. in Journal of Biomedical Materials Research - Part B Applied Biomaterials Hoboken, NJ : John Wiley & Sons., 94(1), 108-117. https://doi.org/10.1002/jbm.b.31630 https://hdl.handle.net/21.15107/rcub_dais_3419
Ignjatović N, Ajduković Z, Savić VP, Uskoković D. Size effect of calcium phosphate coated with poly-DL-lactideco-glycolide on healing processes in bone reconstruction. in Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2010;94(1):108-117. doi:10.1002/jbm.b.31630 https://hdl.handle.net/21.15107/rcub_dais_3419 .
Ignjatović, Nenad, Ajduković, Zorica, Savić, Vojin. P., Uskoković, Dragan, "Size effect of calcium phosphate coated with poly-DL-lactideco-glycolide on healing processes in bone reconstruction" in Journal of Biomedical Materials Research - Part B Applied Biomaterials, 94, no. 1 (2010):108-117, https://doi.org/10.1002/jbm.b.31630 ., https://hdl.handle.net/21.15107/rcub_dais_3419 .