Resveratrol/Selenium Nanocomposite with Antioxidative and Antibacterial Properties
Аутори
Tomić, NinaStevanović, Magdalena
Filipović, Nenad
Ganić, Tea
Nikolić, Biljana
Gajić, Ina
Mitić-Ćulafić, Dragana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this work, we synthesized a new composite material comprised of previously formulated resveratrol nanobelt-like particles (ResNPs) and selenium nanoparticles (SeNPs), namely ResSeNPs. Characterization was provided by FESEM and optical microscopy, as well as by UV-Vis and FTIR spectroscopy, the last showing hydrogen bonds between ResNPs and SeNPs. DPPH, TBA, and FRAP assays showed excellent antioxidative abilities with ResNPs and SeNPs contributing mainly to lipid peroxidation inhibition and reducing/scavenging activity, respectively. The antibacterial effect against common medicinal implant colonizers pointed to notably higher activity against Staphylococcus isolates (minimal inhibitory concentrations 0.75–1.5%) compared to tested gram-negative species (Escherichia coli and Pseudomonas aeruginosa). Antibiofilm activity against S. aureus, S. epidermidis, and P. aeruginosa determined in a crystal violet assay was promising (up to 69%), but monitoring of selected biofilm-related gene e...xpression (pelA and algD) indicated the necessity of the involvement of a larger number of genes in the analysis in order to further establish the underlying mechanism. Although biocompatibility screening showed some cytotoxicity and genotoxicity in MTT and alkaline comet assays, respectively, it is important to note that active antioxidative and antibacterial/antibiofilm concentrations were non-cytotoxic and non-genotoxic in normal MRC-5 cells. These results encourage further composite improvements and investigation in order to adapt it for specific biomedical purposes.
Кључне речи:
antibiofilm / antioxidative / biocompatibility / resveratrol / selenium nanoparticlesИзвор:
Nanomaterials, 2024, 14, 4, 368-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200175 (Институт техничких наука САНУ, Београд) (RS-MESTD-inst-2020-200175)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Tomić, Nina AU - Stevanović, Magdalena AU - Filipović, Nenad AU - Ganić, Tea AU - Nikolić, Biljana AU - Gajić, Ina AU - Mitić-Ćulafić, Dragana PY - 2024 UR - https://dais.sanu.ac.rs/123456789/16566 AB - In this work, we synthesized a new composite material comprised of previously formulated resveratrol nanobelt-like particles (ResNPs) and selenium nanoparticles (SeNPs), namely ResSeNPs. Characterization was provided by FESEM and optical microscopy, as well as by UV-Vis and FTIR spectroscopy, the last showing hydrogen bonds between ResNPs and SeNPs. DPPH, TBA, and FRAP assays showed excellent antioxidative abilities with ResNPs and SeNPs contributing mainly to lipid peroxidation inhibition and reducing/scavenging activity, respectively. The antibacterial effect against common medicinal implant colonizers pointed to notably higher activity against Staphylococcus isolates (minimal inhibitory concentrations 0.75–1.5%) compared to tested gram-negative species (Escherichia coli and Pseudomonas aeruginosa). Antibiofilm activity against S. aureus, S. epidermidis, and P. aeruginosa determined in a crystal violet assay was promising (up to 69%), but monitoring of selected biofilm-related gene expression (pelA and algD) indicated the necessity of the involvement of a larger number of genes in the analysis in order to further establish the underlying mechanism. Although biocompatibility screening showed some cytotoxicity and genotoxicity in MTT and alkaline comet assays, respectively, it is important to note that active antioxidative and antibacterial/antibiofilm concentrations were non-cytotoxic and non-genotoxic in normal MRC-5 cells. These results encourage further composite improvements and investigation in order to adapt it for specific biomedical purposes. T2 - Nanomaterials T1 - Resveratrol/Selenium Nanocomposite with Antioxidative and Antibacterial Properties SP - 368 VL - 14 IS - 4 DO - 10.3390/nano14040368 UR - https://hdl.handle.net/21.15107/rcub_dais_16566 ER -
@article{ author = "Tomić, Nina and Stevanović, Magdalena and Filipović, Nenad and Ganić, Tea and Nikolić, Biljana and Gajić, Ina and Mitić-Ćulafić, Dragana", year = "2024", abstract = "In this work, we synthesized a new composite material comprised of previously formulated resveratrol nanobelt-like particles (ResNPs) and selenium nanoparticles (SeNPs), namely ResSeNPs. Characterization was provided by FESEM and optical microscopy, as well as by UV-Vis and FTIR spectroscopy, the last showing hydrogen bonds between ResNPs and SeNPs. DPPH, TBA, and FRAP assays showed excellent antioxidative abilities with ResNPs and SeNPs contributing mainly to lipid peroxidation inhibition and reducing/scavenging activity, respectively. The antibacterial effect against common medicinal implant colonizers pointed to notably higher activity against Staphylococcus isolates (minimal inhibitory concentrations 0.75–1.5%) compared to tested gram-negative species (Escherichia coli and Pseudomonas aeruginosa). Antibiofilm activity against S. aureus, S. epidermidis, and P. aeruginosa determined in a crystal violet assay was promising (up to 69%), but monitoring of selected biofilm-related gene expression (pelA and algD) indicated the necessity of the involvement of a larger number of genes in the analysis in order to further establish the underlying mechanism. Although biocompatibility screening showed some cytotoxicity and genotoxicity in MTT and alkaline comet assays, respectively, it is important to note that active antioxidative and antibacterial/antibiofilm concentrations were non-cytotoxic and non-genotoxic in normal MRC-5 cells. These results encourage further composite improvements and investigation in order to adapt it for specific biomedical purposes.", journal = "Nanomaterials", title = "Resveratrol/Selenium Nanocomposite with Antioxidative and Antibacterial Properties", pages = "368", volume = "14", number = "4", doi = "10.3390/nano14040368", url = "https://hdl.handle.net/21.15107/rcub_dais_16566" }
Tomić, N., Stevanović, M., Filipović, N., Ganić, T., Nikolić, B., Gajić, I.,& Mitić-Ćulafić, D.. (2024). Resveratrol/Selenium Nanocomposite with Antioxidative and Antibacterial Properties. in Nanomaterials, 14(4), 368. https://doi.org/10.3390/nano14040368 https://hdl.handle.net/21.15107/rcub_dais_16566
Tomić N, Stevanović M, Filipović N, Ganić T, Nikolić B, Gajić I, Mitić-Ćulafić D. Resveratrol/Selenium Nanocomposite with Antioxidative and Antibacterial Properties. in Nanomaterials. 2024;14(4):368. doi:10.3390/nano14040368 https://hdl.handle.net/21.15107/rcub_dais_16566 .
Tomić, Nina, Stevanović, Magdalena, Filipović, Nenad, Ganić, Tea, Nikolić, Biljana, Gajić, Ina, Mitić-Ćulafić, Dragana, "Resveratrol/Selenium Nanocomposite with Antioxidative and Antibacterial Properties" in Nanomaterials, 14, no. 4 (2024):368, https://doi.org/10.3390/nano14040368 ., https://hdl.handle.net/21.15107/rcub_dais_16566 .