Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
2016
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Аутори
Jugović, BranimirGrgur, Branimir
Antov, Mirjana
Knežević Jugović, Zorica
Stevanović, Jasmina S.
Gvozdenović, Milica M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
Кључне речи:
conducting polymers / electrochemistry / glucose oxidase / material testing / sensorsИзвор:
International Journal of Electrochemcal Science, 2016, 11, 2, 1152-1161Финансирање / пројекти:
- Електрохемијска синтеза и карактеризација наноструктурираних функционалних материјала за примену у новим технологијама (RS-MESTD-Basic Research (BR or ON)-172046)
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Jugović, Branimir AU - Grgur, Branimir AU - Antov, Mirjana AU - Knežević Jugović, Zorica AU - Stevanović, Jasmina S. AU - Gvozdenović, Milica M. PY - 2016 UR - https://dais.sanu.ac.rs/123456789/15979 AB - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix. T2 - International Journal of Electrochemcal Science T1 - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose SP - 1152 EP - 1161 VL - 11 IS - 2 UR - https://hdl.handle.net/21.15107/rcub_dais_15979 ER -
@article{ author = "Jugović, Branimir and Grgur, Branimir and Antov, Mirjana and Knežević Jugović, Zorica and Stevanović, Jasmina S. and Gvozdenović, Milica M.", year = "2016", abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.", journal = "International Journal of Electrochemcal Science", title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose", pages = "1152-1161", volume = "11", number = "2", url = "https://hdl.handle.net/21.15107/rcub_dais_15979" }
Jugović, B., Grgur, B., Antov, M., Knežević Jugović, Z., Stevanović, J. S.,& Gvozdenović, M. M.. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemcal Science, 11(2), 1152-1161. https://hdl.handle.net/21.15107/rcub_dais_15979
Jugović B, Grgur B, Antov M, Knežević Jugović Z, Stevanović JS, Gvozdenović MM. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemcal Science. 2016;11(2):1152-1161. https://hdl.handle.net/21.15107/rcub_dais_15979 .
Jugović, Branimir, Grgur, Branimir, Antov, Mirjana, Knežević Jugović, Zorica, Stevanović, Jasmina S., Gvozdenović, Milica M., "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" in International Journal of Electrochemcal Science, 11, no. 2 (2016):1152-1161, https://hdl.handle.net/21.15107/rcub_dais_15979 .