Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin
Само за регистроване кориснике
2020
Аутори
Batinić, Petar M.Đorđević, Verica B.
Stevanović, Sanja
Balanč, Bojana D.
Marković, Smilja B.
Luković, Nevena D.
Mijin, Dušan
Bugarski, Branko
Чланак у часопису (Објављена верзија)
,
Elsevier
Метаподаци
Приказ свих података о документуАпстракт
In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Releas...e study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility.
Кључне речи:
encapsulation efficiency / Phospholipon 90G / cholesterol / SPAN.20 / Franz diffusion cellИзвор:
Journal of Drug Delivery Science and Technology, 2020, 59, 101920-Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-200287)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-200135)
Напомена:
- Peer-reviewed manuscript: https://hdl.handle.net/21.15107/rcub_dais_9517
- Supplementary information: https://hdl.handle.net/21.15107/rcub_dais_9518
Повезане информације:
- Друга верзија
https://hdl.handle.net/21.15107/rcub_dais_9517 - Повезани садржај
https://hdl.handle.net/21.15107/rcub_dais_9518
DOI: 10.1016/j.jddst.2020.101920
ISSN: 1773-2247
WoS: 000572445500003
Scopus: 2-s2.0-85088379189
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Batinić, Petar M. AU - Đorđević, Verica B. AU - Stevanović, Sanja AU - Balanč, Bojana D. AU - Marković, Smilja B. AU - Luković, Nevena D. AU - Mijin, Dušan AU - Bugarski, Branko PY - 2020 UR - https://dais.sanu.ac.rs/123456789/9516 AB - In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Release study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility. PB - Elsevier T2 - Journal of Drug Delivery Science and Technology T1 - Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin SP - 101920 VL - 59 DO - 10.1016/j.jddst.2020.101920 UR - https://hdl.handle.net/21.15107/rcub_dais_9516 ER -
@article{ author = "Batinić, Petar M. and Đorđević, Verica B. and Stevanović, Sanja and Balanč, Bojana D. and Marković, Smilja B. and Luković, Nevena D. and Mijin, Dušan and Bugarski, Branko", year = "2020", abstract = "In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Release study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility.", publisher = "Elsevier", journal = "Journal of Drug Delivery Science and Technology", title = "Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin", pages = "101920", volume = "59", doi = "10.1016/j.jddst.2020.101920", url = "https://hdl.handle.net/21.15107/rcub_dais_9516" }
Batinić, P. M., Đorđević, V. B., Stevanović, S., Balanč, B. D., Marković, S. B., Luković, N. D., Mijin, D.,& Bugarski, B.. (2020). Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin. in Journal of Drug Delivery Science and Technology Elsevier., 59, 101920. https://doi.org/10.1016/j.jddst.2020.101920 https://hdl.handle.net/21.15107/rcub_dais_9516
Batinić PM, Đorđević VB, Stevanović S, Balanč BD, Marković SB, Luković ND, Mijin D, Bugarski B. Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin. in Journal of Drug Delivery Science and Technology. 2020;59:101920. doi:10.1016/j.jddst.2020.101920 https://hdl.handle.net/21.15107/rcub_dais_9516 .
Batinić, Petar M., Đorđević, Verica B., Stevanović, Sanja, Balanč, Bojana D., Marković, Smilja B., Luković, Nevena D., Mijin, Dušan, Bugarski, Branko, "Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin" in Journal of Drug Delivery Science and Technology, 59 (2020):101920, https://doi.org/10.1016/j.jddst.2020.101920 ., https://hdl.handle.net/21.15107/rcub_dais_9516 .