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dc.creatorIgnjatović, Nenad
dc.creatorPenov Gaši, Katarina
dc.creatorWu, Victoria
dc.creatorAjduković, Jovana
dc.creatorKojić, Vesna
dc.creatorVasiljević Radović, Dana
dc.creatorUskoković, Vuk
dc.creatorUskoković, Dragan
dc.date.accessioned2017-06-10T15:45:07Z
dc.date.issued2016
dc.identifier.isbn978-86-919111-1-9
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/896
dc.description.abstractThe applicative potential of synthetic calcium phosphates, especially hydroxyapatite (HAp), has become intensely broadened in the past 10 years, from bone tissue engineering to multiple other fields of biomedicine. Previously we have shown that hydroxyapatite nanoparticles coated with chitosan-poly(D,L)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous administration into mice. For this purpose radioactive 125-Iodine (125I), a low energy gamma emitter, was used to develop a novel in situ method for radiolabeling of particles and investigation of their biodistribution. In this study we utilize an emulsification process and freeze drying to load the composite particles based on hydroxyapatite nanocarrier, chitosane and poly(lactic-co-glycolic acid) with 17β- hydroxy-17α-picolyl-androst-5-en-3β-acetate (A), a chemotherapeutic derivative of androstane. The picolyl androstane derivatives showed high potency in the cell inhibitors of hormonedependent cancers (adenocarcinoma, prostate cancer, cervix carcinoma, colon cancer, etc.). 1H NMR, 13C NMR and high-resolution time-of-flight mass spectrometry (MS) techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The synthesized particles of A-loaded HAp/Ch-PLGA were found to be spherical in shape with a uniform size distribution of d50=168 nm. The release of A from HAp/Ch-PLGA was sustained, with no burst release or plateauing after three weeks. The obtained results of the DET and MTT tests show that the particles of A-loaded HAp/Ch-PLGA exhibit almost three times higher cytotoxicity towards lung adenocarcinoma cells (A549) than towards healthy cells (MRC5), while at the same time allowing twice as fast recovery of healthy cells. We have also analyzed the period of recovery of healthy, as well as cancer cells, following the treatment with A-loaded HAp/Ch-PLGA. After treatment with A-loaded HAp/Ch-PLGA, healthy cells recover twice as fast as the malignant ones. Immunofluorescent staining of primary fibroblasts interacting with HAp/Ch-PLGA and A-HAp/Ch-PLGA particles demonstrates no negative morphological or proliferative effects on cells.en
dc.format(2016) 27-27
dc.formatapplication/pdf
dc.languageen
dc.publisherBelgrade : Materials Research Society of Serbia
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.rightsopenAccess
dc.sourceProgramme and The Book of Abstracts / Eighteenth Annual Conference YUCOMAT 2016, Herceg Novi, September 5-10, 2016en
dc.subjecthydroxyapatite
dc.subjectchitosan
dc.subjectandrostane
dc.subjectPLGA
dc.subjectnanocarriers
dc.titleTumor-selective hybrid system based on hydroxyapatite nanocarrier, chitosan, poly(lactic-co-glycolic acid) and androstan derivateen
dc.typeconferenceObject
dc.rights.licenseBY-NC-ND
dcterms.abstractПенов Гаши, Катарина; Wу, Вицториа; Aјдуковић, Јована; Којић, Весна; Васиљевић Радовић, Дана; Ускоковић, Вук; Ускоковић, Драган; Игњатовић, Ненад;
dc.citation.spage27
dc.citation.epage27
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/handle/123456789/896/Ignjatovic_Yucomat2016.pdf


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