Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose
2011
Аутори
Jambrec, DaliborkaJugović, Branimir
Gvozdenović, Milica M.
Knežević Jugović, Zorica
Antov, Mirjana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A growing interest in biosensors for use in medical, environmental and food analysis
has been recognized. Biosensors are devices that transform chemical information, usually the concentration of a specific sample component, into an analytically useful signal. Their selectivity depends on the characteristics of enzyme and biosensors’ response rate and sensitivity on electroconducting polymer used. Glucose oxidase (GOx) is the most widely used enzyme in the field of biosensors because of its high specificity for a commercially important analyte, high turnover number and high stability. On the other side, among the conducting polyheterocyclic polymers, polypyrolle (PPy) is of particular interest because the relatively low oxidation potential of the monomer enables films to be grown from aqueous solutions that are compatible with most of biological elements. The aim of this study was to investigate the possibility of glucose determination using enzyme electrode obtained by immobilization ...of GOx into polypyrolle electrochemically polymerised on platinum electrode. Electrochemical synthesis was performed in 0.5 mol dm-3 HCl and 0.2 mol dm-3 pyrolle at constant current density of 2 mA cm-2. Polypyrolle/enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrolle on platinum electrode. Apparent Michaelis constant was determined and it was found to be 0.045 mmol dm-3, which is much lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into polymer electroconducting matrix. PPy/enzyme electrode lost 5% and 18% of its initial signal after 5 and 20 days, respectively.
Кључне речи:
polypyrolle and glucose oxidase electrode / biosensors / enzyme electrode / electrochemistryИзвор:
Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering, 2011, 1310-1310Издавач:
- Tatranské Matliare : Slovak Society of Chemical Engineering
Финансирање / пројекти:
- Електрохемијска синтеза и карактеризација наноструктурираних функционалних материјала за примену у новим технологијама (RS-MESTD-Basic Research (BR or ON)-172046)
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Jambrec, Daliborka AU - Jugović, Branimir AU - Gvozdenović, Milica M. AU - Knežević Jugović, Zorica AU - Antov, Mirjana PY - 2011 UR - https://dais.sanu.ac.rs/123456789/694 AB - A growing interest in biosensors for use in medical, environmental and food analysis has been recognized. Biosensors are devices that transform chemical information, usually the concentration of a specific sample component, into an analytically useful signal. Their selectivity depends on the characteristics of enzyme and biosensors’ response rate and sensitivity on electroconducting polymer used. Glucose oxidase (GOx) is the most widely used enzyme in the field of biosensors because of its high specificity for a commercially important analyte, high turnover number and high stability. On the other side, among the conducting polyheterocyclic polymers, polypyrolle (PPy) is of particular interest because the relatively low oxidation potential of the monomer enables films to be grown from aqueous solutions that are compatible with most of biological elements. The aim of this study was to investigate the possibility of glucose determination using enzyme electrode obtained by immobilization of GOx into polypyrolle electrochemically polymerised on platinum electrode. Electrochemical synthesis was performed in 0.5 mol dm-3 HCl and 0.2 mol dm-3 pyrolle at constant current density of 2 mA cm-2. Polypyrolle/enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrolle on platinum electrode. Apparent Michaelis constant was determined and it was found to be 0.045 mmol dm-3, which is much lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into polymer electroconducting matrix. PPy/enzyme electrode lost 5% and 18% of its initial signal after 5 and 20 days, respectively. PB - Tatranské Matliare : Slovak Society of Chemical Engineering C3 - Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering T1 - Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose SP - 1310 EP - 1310 UR - https://hdl.handle.net/21.15107/rcub_dais_694 ER -
@conference{ author = "Jambrec, Daliborka and Jugović, Branimir and Gvozdenović, Milica M. and Knežević Jugović, Zorica and Antov, Mirjana", year = "2011", abstract = "A growing interest in biosensors for use in medical, environmental and food analysis has been recognized. Biosensors are devices that transform chemical information, usually the concentration of a specific sample component, into an analytically useful signal. Their selectivity depends on the characteristics of enzyme and biosensors’ response rate and sensitivity on electroconducting polymer used. Glucose oxidase (GOx) is the most widely used enzyme in the field of biosensors because of its high specificity for a commercially important analyte, high turnover number and high stability. On the other side, among the conducting polyheterocyclic polymers, polypyrolle (PPy) is of particular interest because the relatively low oxidation potential of the monomer enables films to be grown from aqueous solutions that are compatible with most of biological elements. The aim of this study was to investigate the possibility of glucose determination using enzyme electrode obtained by immobilization of GOx into polypyrolle electrochemically polymerised on platinum electrode. Electrochemical synthesis was performed in 0.5 mol dm-3 HCl and 0.2 mol dm-3 pyrolle at constant current density of 2 mA cm-2. Polypyrolle/enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrolle on platinum electrode. Apparent Michaelis constant was determined and it was found to be 0.045 mmol dm-3, which is much lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into polymer electroconducting matrix. PPy/enzyme electrode lost 5% and 18% of its initial signal after 5 and 20 days, respectively.", publisher = "Tatranské Matliare : Slovak Society of Chemical Engineering", journal = "Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering", title = "Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose", pages = "1310-1310", url = "https://hdl.handle.net/21.15107/rcub_dais_694" }
Jambrec, D., Jugović, B., Gvozdenović, M. M., Knežević Jugović, Z.,& Antov, M.. (2011). Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose. in Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering Tatranské Matliare : Slovak Society of Chemical Engineering., 1310-1310. https://hdl.handle.net/21.15107/rcub_dais_694
Jambrec D, Jugović B, Gvozdenović MM, Knežević Jugović Z, Antov M. Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose. in Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering. 2011;:1310-1310. https://hdl.handle.net/21.15107/rcub_dais_694 .
Jambrec, Daliborka, Jugović, Branimir, Gvozdenović, Milica M., Knežević Jugović, Zorica, Antov, Mirjana, "Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose" in Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering (2011):1310-1310, https://hdl.handle.net/21.15107/rcub_dais_694 .