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dc.creatorCatanzaro, Valeria
dc.creatorDigilio, Giuseppe
dc.creatorCapuana, Federico
dc.creatorPadovan, Sergio
dc.creatorCutrin, Juan C.
dc.creatorCarniato, Fabio
dc.creatorPorta, Stefano
dc.creatorGrange, Cristina
dc.creatorFilipović, Nenad
dc.creatorStevanović, Magdalena
dc.date.accessioned2019-11-25T13:21:51Z
dc.date.available2019-11-25T13:21:51Z
dc.date.issued2019
dc.identifier.isbn2079-4983
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/6689
dc.description.abstractCell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200–600 µm diameter and 50–80 µm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T1-weighted (T1w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progressive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs. © 2019 by the authors.
dc.publisherBasel : MDPI
dc.relationMinistry of Foreign Affairs and International Cooperation (Research Project of Particular Relevance between Italy and Serbia—PGR02952)
dc.relationItalian Ministry of University and Education (PRIN-2010 n. 2010B5B2NL)
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.rightsopenAccess
dc.sourceJournal of Functional Biomaterials
dc.subjectBiomaterial
dc.subjectCell scaffold
dc.subjectGadolinium
dc.subjectGraft transplantation
dc.subjectHuman mesenchymal stromal cells (hMSC)
dc.subjectImmune response
dc.subjectMagnetic Resonance Imaging
dc.titleGadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging
dc.typearticleen
dc.rights.licenseBY
dcterms.abstractЦутрин, Јуан Ц.; Стевановић, Магдалена; Филиповић, Ненад; Гранге, Цристина; Порта, Стефано; Царниато, Фабио; Падован, Сергио; Цапуана, Федерицо; Дигилио, Гиусеппе; Цатанзаро, Валериа;
dc.citation.spage28
dc.citation.volume10
dc.citation.issue3
dc.identifier.doi10.3390/jfb10030028
dc.identifier.scopus2-s2.0-85071891444
dc.description.otherSupporting information: [http://dais.sanu.ac.rs/123456789/7044]
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/id/26808/Catanzaro_Journal-of-Functional-Biomaterials_2019.pdf


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