In-vitro visualization of primary tumor cells using up-conversion nanophosphors
Đukić Vuković, Aleksandra
Rabasović, Mihailo D.
Costa, Antonio Mario Leal Martins
Conference object (Published version)
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For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er@PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a bi...ological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.
Keywords:visualization / tumor cells / up-conversion / nanophosphors / X-ray powder diffraction (XRPD) / energy dispersive X-ray (EDX) / Fourier transform infrared spectroscopy (FTIR) / photoluminescence spectroscopy (PL) / FESEM/TEM
Source:Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017, 2017, 59-59
- Belgrade : Serbian Ceramic Society