Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
Само за регистроване кориснике
2014
Аутори
Stupar, PetarPavlović, Vladimir B.
Nunić, Jana
Cundrič, Sandra
Filipič, Metka
Stevanović, Magdalena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytoto...xicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
Кључне речи:
poly(epsilon- caprolactone) / reactive oxygen species / freeze drying / physicochemical solvent and non-solvent approachИзвор:
Journal of Drug Delivery Science and Technology, 2014, 24, 2, 191-197Издавач:
- Paris : Association de pharmacie galenique industrielle
Финансирање / пројекти:
DOI: 10.1016/S1773-2247(14)50031-7
ISSN: 1773-2247
WoS: 000339928800011
Scopus: 2-s2.0-84899567719
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Stupar, Petar AU - Pavlović, Vladimir B. AU - Nunić, Jana AU - Cundrič, Sandra AU - Filipič, Metka AU - Stevanović, Magdalena PY - 2014 UR - https://dais.sanu.ac.rs/123456789/4671 AB - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes. PB - Paris : Association de pharmacie galenique industrielle T2 - Journal of Drug Delivery Science and Technology T1 - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species SP - 191 EP - 197 VL - 24 IS - 2 DO - 10.1016/S1773-2247(14)50031-7 UR - https://hdl.handle.net/21.15107/rcub_dais_4671 ER -
@article{ author = "Stupar, Petar and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Stevanović, Magdalena", year = "2014", abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.", publisher = "Paris : Association de pharmacie galenique industrielle", journal = "Journal of Drug Delivery Science and Technology", title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species", pages = "191-197", volume = "24", number = "2", doi = "10.1016/S1773-2247(14)50031-7", url = "https://hdl.handle.net/21.15107/rcub_dais_4671" }
Stupar, P., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Stevanović, M.. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology Paris : Association de pharmacie galenique industrielle., 24(2), 191-197. https://doi.org/10.1016/S1773-2247(14)50031-7 https://hdl.handle.net/21.15107/rcub_dais_4671
Stupar P, Pavlović VB, Nunić J, Cundrič S, Filipič M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology. 2014;24(2):191-197. doi:10.1016/S1773-2247(14)50031-7 https://hdl.handle.net/21.15107/rcub_dais_4671 .
Stupar, Petar, Pavlović, Vladimir B., Nunić, Jana, Cundrič, Sandra, Filipič, Metka, Stevanović, Magdalena, "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species" in Journal of Drug Delivery Science and Technology, 24, no. 2 (2014):191-197, https://doi.org/10.1016/S1773-2247(14)50031-7 ., https://hdl.handle.net/21.15107/rcub_dais_4671 .