Regeneration bone tissue by new nanoparticules system based on hydroxiapatite as systems for local delivery of vitamin D3

Abstract

Increased life expectancy in developed countries leads to an increase in the number of musculoskeletal disorders, such as osteoporosis, oasteoratritis thus compromising good dental treatment. There are many drug delivery systems based on hydroxyapatite used in bone tissue regeneration. Vitamin D3 is osteodiferentiation factor which regulates bone formation by increasing osteoblast differentiation and bone mineralization. The aim of this study is to examine new multifunctional nanoparticulate system for local delivery of active form of vitamin D3 by biochemical blood marker and histochemical analysis. The research was carried out on female Westar rats, aged 6-8 weeks, which have been implanted biomaterials in the artificial bone defect. Biochemical markers of osteogenesis were statistically significant after only 6 weeks of implantation. ALP activity in bone tissue was showed by histochemical analysis as well as high level reparatory skills. Local realized Vitamin D3 contribute to bone formation by increasing osteoblast differentiation and bone mineralization.