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dc.creatorStupar, Petar
dc.creatorPavlović, Vladimir B.
dc.creatorNunić, Jana
dc.creatorCundrič, Sandra
dc.creatorFilipič, Metka
dc.creatorStevanović, Magdalena
dc.date.accessioned2017-06-10T15:45:07Z
dc.date.available2015-12-17T15:45:07Z
dc.date.issued2014
dc.identifier.issn1773-2247
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/425
dc.description.abstractA common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.en
dc.format24 2 (2014) 191-197
dc.formatapplication/pdf
dc.languageen
dc.publisherParis : Association de pharmacie galenique industrielle
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.rightsembargoedAccess
dc.sourceJournal of Drug Delivery Science and Technologyen
dc.subjectpoly(epsilon- caprolactone)
dc.subjectreactive oxygen species
dc.subjectfreeze drying
dc.subjectphysicochemical solvent and non-solvent approach
dc.titleDevelopment of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen speciesen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractСтевановић, Магдалена; Нунић, Јана; Павловић, Владимир Б.; Ступар, Петар; Цундрич, Сандра; Филипич, Метка;
dc.citation.spage191
dc.citation.epage197
dc.citation.volume24
dc.citation.issue2
dc.identifier.wos000339928800011
dc.identifier.doi10.1016/S1773-2247(14)50031-7
dc.identifier.scopus2-s2.0-84899567719
dc.type.versionacceptedVersion


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