Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
Аутори
Stupar, PetarPavlović, Vladimir B.
Nunić, Jana
Cundrič, Sandra
Filipič, Metka
Stevanović, Magdalena
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytoto...xicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
Кључне речи:
poly(epsilon- caprolactone) / reactive oxygen species / freeze drying / physicochemical solvent and non-solvent approachИзвор:
Journal of Drug Delivery Science and Technology, 2014, 24, 2, 191-197Издавач:
- Paris : Association de pharmacie galenique industrielle
Финансирање / пројекти:
DOI: 10.1016/S1773-2247(14)50031-7
ISSN: 1773-2247
WoS: 000339928800011
Scopus: 2-s2.0-84899567719
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Stupar, Petar AU - Pavlović, Vladimir B. AU - Nunić, Jana AU - Cundrič, Sandra AU - Filipič, Metka AU - Stevanović, Magdalena PY - 2014 UR - https://dais.sanu.ac.rs/123456789/425 AB - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes. PB - Paris : Association de pharmacie galenique industrielle T2 - Journal of Drug Delivery Science and Technology T1 - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species SP - 191 EP - 197 VL - 24 IS - 2 DO - 10.1016/S1773-2247(14)50031-7 UR - https://hdl.handle.net/21.15107/rcub_dais_425 ER -
@article{ author = "Stupar, Petar and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Stevanović, Magdalena", year = "2014", abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.", publisher = "Paris : Association de pharmacie galenique industrielle", journal = "Journal of Drug Delivery Science and Technology", title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species", pages = "191-197", volume = "24", number = "2", doi = "10.1016/S1773-2247(14)50031-7", url = "https://hdl.handle.net/21.15107/rcub_dais_425" }
Stupar, P., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Stevanović, M.. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology Paris : Association de pharmacie galenique industrielle., 24(2), 191-197. https://doi.org/10.1016/S1773-2247(14)50031-7 https://hdl.handle.net/21.15107/rcub_dais_425
Stupar P, Pavlović VB, Nunić J, Cundrič S, Filipič M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. in Journal of Drug Delivery Science and Technology. 2014;24(2):191-197. doi:10.1016/S1773-2247(14)50031-7 https://hdl.handle.net/21.15107/rcub_dais_425 .
Stupar, Petar, Pavlović, Vladimir B., Nunić, Jana, Cundrič, Sandra, Filipič, Metka, Stevanović, Magdalena, "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species" in Journal of Drug Delivery Science and Technology, 24, no. 2 (2014):191-197, https://doi.org/10.1016/S1773-2247(14)50031-7 ., https://hdl.handle.net/21.15107/rcub_dais_425 .