One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for: In vitro cell imaging
Đukić Vuković, Aleksandra
Nikolić, Marko G.
Rabasović, Mihailo D.
Costa, Antonio Mario Leal Martins
Marinković, Bojan A.
Article (Published version)
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The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic α phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (2H11/2, 4S3/2 → 4I15/2) and stronger red emission (4F9/2 → 4I15/2), as a result of enhanced non-radiative 4I1...1/2 → 4I13/2 Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 μg ml-1, without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma. © 2018 The Royal Society of Chemistry.
Keywords:upconversion / nanoparticles / cell imaging / NaYF4:Yb,Er
Source:RSC Advances, 2018, 8, 48, 27429-27437
- London : Royal Society of Chemistry
- Fabrication and characterization of nano-photonic functional structrues in biomedicine and informatics (RS-45016)
- Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology (RS-172035)
- Supporting information: http://dais.sanu.ac.rs/123456789/3785