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The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites
dc.creator | Ignjatović, Nenad | |
dc.creator | Penov Gaši, Katarina | |
dc.creator | Ajduković, Jovana | |
dc.creator | Kojić, Vesna V. | |
dc.creator | Marković, Smilja | |
dc.creator | Uskoković, Dragan | |
dc.date.accessioned | 2018-09-04T12:51:09Z | |
dc.date.available | 2018-09-04T12:51:09Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0928-4931 | |
dc.identifier.uri | https://dais.sanu.ac.rs/123456789/3699 | |
dc.description.abstract | An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was examined simultaneously on two cell lines: human lung carcinoma (A549 ATCC CCL 185) and human lung fibroblasts (MRC-5 ATCC CCL 171). All particles exhibited nearly three times larger cytotoxicity towards cancer cells (A549) than towards healthy cells (MRC5), where the particles with the derivative A content of 6.5 wt% allowed for the viability of healthy cells >80%. Ninety-six hours after the treatment of cells with particles with different contents of derivative A (after incubation and recovery), recovery was faster in damaged healthy cells than in cancerous cells. © 2018 Elsevier B.V. | en |
dc.publisher | Elsevier | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS// | |
dc.rights | restrictedAccess | |
dc.source | Materials Science and Engineering C | |
dc.subject | androstane | |
dc.subject | cell-selective cytotoxicity | |
dc.subject | hydroxyapatite | |
dc.subject | lung cancer | |
dc.subject | nanocarriers | |
dc.title | The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites | en |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Игњатовић, Ненад; Aјдуковић, Јована; Којић, Весна В.; Марковић, Смиља; Пенов-Гаши, К.М.; Ускоковић, Драган; | |
dc.citation.spage | 371 | |
dc.citation.epage | 377 | |
dc.description.note | Accepted version: [https://hdl.handle.net/21.15107/rcub_dais_4067] | |
dc.citation.volume | 89 | |
dc.identifier.wos | 000434239100039 | |
dc.identifier.doi | 10.1016/j.msec.2018.04.028 | |
dc.identifier.scopus | 2-s2.0-85045579234 | |
dc.type.version | publishedVersion | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_dais_3699 |