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dc.creatorIgnjatović, Nenad
dc.creatorPenov Gaši, Katarina
dc.creatorAjduković, Jovana
dc.creatorKojić, Vesna V.
dc.creatorMarković, Smilja
dc.creatorUskoković, Dragan
dc.date.accessioned2018-09-04T12:51:09Z
dc.date.available2018-09-04T12:51:09Z
dc.date.issued2018
dc.identifier.issn0928-4931
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/3699
dc.description.abstractAn androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was examined simultaneously on two cell lines: human lung carcinoma (A549 ATCC CCL 185) and human lung fibroblasts (MRC-5 ATCC CCL 171). All particles exhibited nearly three times larger cytotoxicity towards cancer cells (A549) than towards healthy cells (MRC5), where the particles with the derivative A content of 6.5 wt% allowed for the viability of healthy cells >80%. Ninety-six hours after the treatment of cells with particles with different contents of derivative A (after incubation and recovery), recovery was faster in damaged healthy cells than in cancerous cells. © 2018 Elsevier B.V.
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.rightsrestrictedAccess
dc.sourceMaterials Science and Engineering C
dc.subjectandrostane
dc.subjectcell-selective cytotoxicity
dc.subjecthydroxyapatite
dc.subjectlung cancer
dc.subjectnanocarriers
dc.titleThe effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites
dc.typearticleen
dc.rights.licenseARR
dcterms.abstractИгњатовић, Ненад; Aјдуковић, Јована; Којић, Весна В.; Марковић, Смиља; Пенов-Гаши, К.М.; Ускоковић, Драган;
dc.citation.spage371
dc.citation.epage377
dc.description.noteAccepted version: [http://dais.sanu.ac.rs/123456789/4067]
dc.citation.volume89
dc.identifier.wos000434239100039
dc.identifier.doi10.1016/j.msec.2018.04.028
dc.identifier.scopus2-s2.0-85045579234
dc.type.versionpublishedVersion


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