Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres
Samo za registrovane korisnike
2009
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this work, horseradish peroxidase (HRP), a model protein, was encapsulated by the precipitation method with additional step of homogenization, using polymer poly(D,L-lactide) (PDLLA). The purpose of this study was to expand the application of a modified precipitation method to HRP-loaded PDLLA spheres and to find optimal conditions for preparing HRP-loaded PDLLA spheres with the best properties, with size under 1000 nm. Influence of process variables on the size and morphology of the particles has been studied. Size of the spheres depends on homogenization speed, co-solvent selection, chloroform to water ratio and PVA concentration. Particle size was ranging between 460 nm and 1130 nm, according to the preparation conditions. The optimized conditions yielded nanospheres with 46% encapsulation efficiency and average diameter of 530 nm. A particle size under 1000 nm enables intravascular injection and is also desirable for intramuscular and subcutaneous administration, minimizing poss...ible irritant reactions. It was found that modified precipitation method was widely applicable to HRP-loaded PDLLA spheres, by choosing optimal preparation conditions. Copyright © 2009 American Scientific Publishers All rights reserved.
Ključne reči:
encapsulation / horseradish peroxidase / poly(D,L-lactide) nanospheres / precipitation / protein stabilityIzvor:
Journal of Bionanoscience, 2009, 3, 1, 22-32Izdavač:
- American Scientific Publishers
Finansiranje / projekti:
- Sinteza funkcionalnih materijala sa kontrolisanom strukturom na molekularnom i nano nivou (RS-142006)
Institucija/grupa
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Mitranić, Ivana AU - Stevanović, Magdalena AU - Nedeljković, B. AU - Ignjatović, Nenad AU - Uskoković, Dragan PY - 2009 UR - https://dais.sanu.ac.rs/123456789/3476 AB - In this work, horseradish peroxidase (HRP), a model protein, was encapsulated by the precipitation method with additional step of homogenization, using polymer poly(D,L-lactide) (PDLLA). The purpose of this study was to expand the application of a modified precipitation method to HRP-loaded PDLLA spheres and to find optimal conditions for preparing HRP-loaded PDLLA spheres with the best properties, with size under 1000 nm. Influence of process variables on the size and morphology of the particles has been studied. Size of the spheres depends on homogenization speed, co-solvent selection, chloroform to water ratio and PVA concentration. Particle size was ranging between 460 nm and 1130 nm, according to the preparation conditions. The optimized conditions yielded nanospheres with 46% encapsulation efficiency and average diameter of 530 nm. A particle size under 1000 nm enables intravascular injection and is also desirable for intramuscular and subcutaneous administration, minimizing possible irritant reactions. It was found that modified precipitation method was widely applicable to HRP-loaded PDLLA spheres, by choosing optimal preparation conditions. Copyright © 2009 American Scientific Publishers All rights reserved. PB - American Scientific Publishers T2 - Journal of Bionanoscience T1 - Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres SP - 22 EP - 32 VL - 3 IS - 1 DO - 10.1166/jbns.2009.1005 UR - https://hdl.handle.net/21.15107/rcub_dais_3476 ER -
@article{ author = "Mitranić, Ivana and Stevanović, Magdalena and Nedeljković, B. and Ignjatović, Nenad and Uskoković, Dragan", year = "2009", abstract = "In this work, horseradish peroxidase (HRP), a model protein, was encapsulated by the precipitation method with additional step of homogenization, using polymer poly(D,L-lactide) (PDLLA). The purpose of this study was to expand the application of a modified precipitation method to HRP-loaded PDLLA spheres and to find optimal conditions for preparing HRP-loaded PDLLA spheres with the best properties, with size under 1000 nm. Influence of process variables on the size and morphology of the particles has been studied. Size of the spheres depends on homogenization speed, co-solvent selection, chloroform to water ratio and PVA concentration. Particle size was ranging between 460 nm and 1130 nm, according to the preparation conditions. The optimized conditions yielded nanospheres with 46% encapsulation efficiency and average diameter of 530 nm. A particle size under 1000 nm enables intravascular injection and is also desirable for intramuscular and subcutaneous administration, minimizing possible irritant reactions. It was found that modified precipitation method was widely applicable to HRP-loaded PDLLA spheres, by choosing optimal preparation conditions. Copyright © 2009 American Scientific Publishers All rights reserved.", publisher = "American Scientific Publishers", journal = "Journal of Bionanoscience", title = "Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres", pages = "22-32", volume = "3", number = "1", doi = "10.1166/jbns.2009.1005", url = "https://hdl.handle.net/21.15107/rcub_dais_3476" }
Mitranić, I., Stevanović, M., Nedeljković, B., Ignjatović, N.,& Uskoković, D.. (2009). Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres. in Journal of Bionanoscience American Scientific Publishers., 3(1), 22-32. https://doi.org/10.1166/jbns.2009.1005 https://hdl.handle.net/21.15107/rcub_dais_3476
Mitranić I, Stevanović M, Nedeljković B, Ignjatović N, Uskoković D. Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres. in Journal of Bionanoscience. 2009;3(1):22-32. doi:10.1166/jbns.2009.1005 https://hdl.handle.net/21.15107/rcub_dais_3476 .
Mitranić, Ivana, Stevanović, Magdalena, Nedeljković, B., Ignjatović, Nenad, Uskoković, Dragan, "Controllable synthesis of horseradish peroxidase loaded poly(D,L-lactide) nanospheres" in Journal of Bionanoscience, 3, no. 1 (2009):22-32, https://doi.org/10.1166/jbns.2009.1005 ., https://hdl.handle.net/21.15107/rcub_dais_3476 .