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Ektopična osteogeneza i hematopoeza iz implantiranih ćelija koštane srži na matrici od biokompozita HAP/PLLA

dc.creatorVasiljević, Perica
dc.creatorNajman, Stevo
dc.creatorĐorđević, Ljubiša
dc.creatorSavić, Vojin P.
dc.creatorVukelić, Marija Đ.
dc.creatorŽivanov Čurlis, Jelena Z.
dc.creatorIgnjatović, Nenad
dc.creatorUskoković, Dragan
dc.date.accessioned2018-06-09T11:09:17Z
dc.date.available2018-06-09T11:09:17Z
dc.date.issued2009
dc.identifier.issn0367-598X
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/0367-598X0904301V
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/3362
dc.description.abstractBone tissue reconstruction and reparation is a big challenge in medicine. Biocomposite materials based on hidroxyapatite are widely used in reparation of bone defects. Adult bone marrow-derived stem cells may be considered in two categories: hematopoietic stem cells (HSC) from the bone marrow and mesenchymal stem cells from the bone marrow stroma (BMSC). HSC and BMSC do not only coexist in one organ, but functionally cooperate. BMSC have a critical role in the formation of hematopoietic microenvironment (HME). The aim of this study was to investigate the interactions between bone marrow cells and biocomposites based on HAp/PLLA (hidroxyapatite/poly-L-lactide) after subcutaneous implantation in Balb/c mice. In that purpose, bone marrow cells of Balb/c mice were seeded in HAp/PLLA tubes (15 mm×1,5 mm). The HAp/PLLA tubes with BMC was subcutaneously implanted with a needle into the intrascapsular region of the mouse. Implants were extracted after 2, 6 and 12 weeks. In implants after 2 and 6 weeks we found angiogenesis, collagenogenesis and new bone. Ectopic hematopoiesis was seen in implants after 12 weeks from implantation. As a good scaffold in the role of supporting osteogenesis and hematopoiesis, biocomposites HAp/PLLA can be good bone substitute materials in the bone reparation process. These results showed that the HAp/PLLA scaffold owned biological properties comparable to natural bone.
dc.description.abstractIspitivana je biofunkcionalnost biokompozita hidroksiapatit/poli-L-laktida (HAp/ /PLLA) kao matrice za osteogenezu i hematopoezu posle subkutane implantacije singenim miševima Balb/c. HAp/PLLA oblikovan je u cevčice (15 mm×1,5 mm) koje su napunjene ćelijama koštane srži miša, a zatim implantirane. Kontrole su bile prokuvane tibije i femuri napunjene na isti način. Implanti su ekstrahovani posle 2, 6 i 12 nedelja i analizirani patohistološki. Angiogeneza i osteogeneza se vide već posle dve nedelje na implantima, što je izraženije na kostima. Vrlo izražena hematopoeza u implantima oba tipa se vidi posle dvanaest nedelja. HAp/PLLA može poslužiti kao matrica za ektopičnu osteogenezu i hematopoezu.
dc.publisherBelgrade : Association of the Chemical Engineers of Serbia
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/145072/RS//
dc.rightsopenAccess
dc.sourceHemijska industrija
dc.titleEctopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold
dc.titleEktopična osteogeneza i hematopoeza iz implantiranih ćelija koštane srži na matrici od biokompozita HAP/PLLA
dc.typearticleen
dc.rights.licenseBY-NC-ND
dcterms.abstractСавић, Војин П.; Живанов-Чурлис, Јелена З.; Вукелић, Марија Ђ.; Ускоковић, Драган; Васиљевић, Перица; Игњатовић, Ненад; Најман, Стево; Ђорђевић, Љубиша; Ектопична остеогенеза и хематопоеза из имплантираних ћелија коштане сржи на матрици од биокомпозита ХAП/ПЛЛA;
dc.citation.spage301
dc.citation.epage307
dc.citation.volume63
dc.citation.issue4
dc.identifier.wos000271316600004
dc.identifier.doi10.2298/HEMIND0904301V
dc.identifier.scopus2-s2.0-77952945230
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/id/10703/0367-598X0904301V.pdf


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