Приказ основних података о документу

dc.creatorFilipović, Nenad
dc.creatorStevanović, Magdalena
dc.creatorNunić, Jana
dc.creatorCundrič, Sandra
dc.creatorFilipič, Metka
dc.creatorUskoković, Dragan
dc.date.accessioned2017-06-10T15:45:07Z
dc.date.issued2014
dc.identifier.issn0927-7765
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/330
dc.description.abstractNanospheres of poly (ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.en
dc.format(2014) -
dc.formatapplication/pdf
dc.languageen
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.rightsopenAccess
dc.sourceColloids and Surfaces B: Biointerfaces
dc.subjectpoly(glutamic acid)
dc.subjectcryoprotectants
dc.subjectpoly(ɛ-caprolactone)
dc.subjectnanospheres
dc.subjectbiocompatibility
dc.titleSynthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cellsen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractФилиповић, Ненад; Ускоковић, Драган; Филипич, Метка; Цундрич, Сандра; Нунић, Јана; Стевановић, Магдалена;
dc.citation.spage414
dc.citation.epage424
dc.citation.volume117
dc.citation.issue1
dc.identifier.wos000336018700055
dc.identifier.doi10.1016/j.colsurfb.2014.03.015
dc.identifier.scopus2-s2.0-84897031105
dc.description.otherThis is the peer-reviewed version of the article Filipović, N., Stevanović, M., Nunić, J., Cundrič, S., Filipič, M., Uskoković, D., 2014. Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. Colloids and Surfaces B: Biointerfaces 117, 414–424. [http://dx.doi.org/10.1016/j.colsurfb.2014.03.015]
dc.type.versionacceptedVersion
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/handle/123456789/330/rad-N.Filipovic.pdf


Документи

Thumbnail

Овај рад се појављује у следећим колекцијама

Приказ основних података о документу