Show simple item record

dc.creatorStevanović, Magdalena
dc.creatorMaksin, Tatjana
dc.creatorPetković, Jana
dc.creatorFilipič, Metka
dc.creatorUskoković, Dragan
dc.date.accessioned2018-03-01T20:52:27Z
dc.date.accessioned2018-05-08T07:06:40Z
dc.date.available2018-03-01T20:52:27Z
dc.date.available2018-05-08T07:06:40Z
dc.date.issued2009
dc.identifier.issn0957-4484 (print)
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/2740
dc.description.abstractNanoparticles of poly(DL-lactide-co-glycolide) (PLGA) in the size range 90-150 nm were produced using the physicochemical method with solvent/non-solvent systems. The encapsulation of the ascorbic acid in the polymer matrix was performed by homogenization of the water and organic phases. In vitro degradation and release tests of PLGA nanoparticles with and without encapsulated ascorbic acid were studied for more than 60 days in PBS and it has been determined that PLGA completely degrades within this period, fully releasing all encapsulated ascorbic acid. The cytotoxicity of PLGA and PLGA/ascorbic acid 85/15% nanoparticles was examined with human hepatoma cell lines (HepG2 ECACC), in vitro. The obtained results indicate that neither PLGA nanospheres nor PLGA/ascorbic acid 85/15% nanoparticles significantly affected the viability of the HepG2 cells. The investigation of the distribution and pharmacokinetics of PLGA is crucial for the effective prediction of host responses to PLGA in particular applications. Thus we present a method of labeling PLGA nanospheres and PLGA/ascorbic acid 85/15 wt% nanoparticles by (99m)Tc which binds outside, leaving the cage intact. This enables a quick and convenient investigation of the pharmacological behavior and metabolism of PLGA. The biodistribution of (99m)Tc-labeled PLGA particles with and without encapsulated ascorbic acid after different periods of time of their installation into rats was examined. PLGA nanospheres with encapsulated ascorbic acid exhibit prolonged blood circulation accompanied by time-dependent reduction in the lungs, liver and spleen, and addition in the kidney, stomach and intestine. The samples were characterized by x-ray diffraction, scanning electron microscopy, stereological analysis, transmission electron microscopy, ultraviolet spectroscopy and instant thin layer chromatography.en
dc.publisherBristol : IOP Science
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142006/RS//
dc.rightsrestrictedAccessen
dc.sourceNanotechnologyen
dc.titleAn innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheresen
dc.typearticleen
dcterms.abstractМаксин, Татјана; Петковић, Јана; Ускоковић, Драган; Стевановић, Магдалена; Филипич, Метка;
dc.citation.volume20
dc.citation.issue33
dc.identifier.wos000268480500003
dc.identifier.doi10.1088/0957-4484/20/33/335102
dc.identifier.pmid19636100
dc.identifier.scopus2-s2.0-70249129749
dc.type.versionpublishedVersion
dc.citation.otherArticle Number: 335102


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record