Show simple item record

dc.creatorDinić, Miroslav
dc.creatorPecikoza, Uroš
dc.creatorĐokić, Jelena
dc.creatorStepanović Petrović, Radica
dc.creatorMilenković, Marina
dc.creatorStevanović, Magdalena
dc.creatorFilipović, Nenad
dc.creatorBegović, Jelena
dc.creatorGolić, Nataša
dc.creatorLukić, Jovanka
dc.date.accessioned2018-04-11T10:12:09Z
dc.date.available2018-04-11T10:12:09Z
dc.date.issued2018
dc.identifier.issn1663-9812 (Print)
dc.identifier.issn1663-9812 (Online)
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/2347
dc.description.abstractThe aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1β and iNOS mRNAs in rat’s paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1β, TNF-α and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.en
dc.format9 (2018) Article 1-
dc.formatapplication/pdf
dc.languageen
dc.publisherLausanne : Frontiers
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175045/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.rightsopenAccess
dc.sourceFrontiers in Pharmacologyen
dc.subjectLactobacillus paraplantarum BGCG11
dc.subjectexopolysaccharide
dc.subjectinflammatory hyperalgesia
dc.titleExopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Ratsen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractСтепановић Петровић, Радица; Филиповић, Ненад; Лукић, Јованка; Динић, Мирослав; Голић, Наташа; Миленковић, Марина; Стевановић, Магдалена; Беговић, Јелена; Пецикоза, Урош; Ђокић, Јелена;
dc.citation.spageArticle 1
dc.citation.volume9
dc.identifier.wos000422678300001
dc.identifier.doi10.3389/fphar.2018.00001
dc.identifier.scopus2-s2.0-85041108228
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/handle/123456789/2347/Frontiers in Pharmacology fphar-09-00001.pdf


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record