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dc.creatorRadovanović, Mirjana
dc.creatorJugović, Branimir
dc.creatorGvozdenović, Milica M.
dc.creatorJokić, Bojan
dc.creatorGrgur, Branimir
dc.creatorBugarski, Branko
dc.creatorKnežević Jugović, Zorica
dc.date.accessioned2018-01-30T10:09:10Z
dc.date.available2018-01-30T10:09:10Z
dc.date.issued2016
dc.identifier.issn1521-379X
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/16002
dc.description.abstractThe immobilization of α-amylase via adsorption on magnetic particles coated with polyaniline was studied. The support was characterized by field emission scanning electron microscopy (FESEM). The obtained magnetic particles were agglomerates of nanoparticles with sizes below 100 nm. The effects of various factors on immobilization, including time, the initial enzyme concentration, pH, and temperature, were examined. The optimum pH, temperature, and time for immobilization were established to be 7, 45°C and 75 min, respectively. The maximum amount of adsorbed α-amylase of 10/100 mg support was determined at the 5 mg/mL enzyme concentration. It appeared that α-amylase was stabilized in terms of pH and temperature by adsorption on magnetic particles coated with polyaniline. The good agreement of the equilibrium data with the Langmuir isotherm model confirmed the monolayer coverage of enzyme molecules on the surface of magnetic particles, and the maximum adsorption capacity was found to be 55.6/100 mg support at 25°C. The biocatalyst retained 55.5 ± 1.63% of its initial activity after nine cycles of reuse in starch hydrolysis at 60°C in a batch reactor. The immobilized enzyme also showed very good storage stability.eng
dc.format68 5-6 (2016) 427-435
dc.languageen
dc.publisherHoboken, NJ : John Wiley & Sons
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsrestrictedAccess
dc.sourceStarcheng
dc.subjectα-amylase
dc.subjectimmobilization
dc.subjectadsorption
dc.subjectmagnetic particles
dc.subjectpolyaniline
dc.titleImmobilization of α-amylase via adsorption on magnetic particles coated with polyanilineeng
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРадовановић, Мирјана; Гвозденовић, Милица; Гргур, Бранимир; Кнежзевић-Југовић, Зорица; Јокић, Бојан; Бугарски, Бранко; Југовић, Бранимир;
dc.citation.spage427
dc.citation.epage435
dc.citation.volume68
dc.citation.issue5-6
dc.identifier.wos000380156500006
dc.identifier.doi10.1002/star.201500161
dc.identifier.scopus2-s2.0-84949255495
dc.type.versionpublishedVersion


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