Show simple item record

dc.creatorStevanović, Magdalena
dc.creatorUskoković, Vuk
dc.creatorFilipović, Miloš
dc.creatorŠkapin, Srečo Davor
dc.creatorUskoković, Dragan
dc.date.accessioned2018-01-30T10:09:12Z
dc.date.available2014-08-28
dc.date.issued2013
dc.identifier.issn1944-8244 (Print)
dc.identifier.issn1944-8252 (Online)
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/15986
dc.description.abstractThe global rise in the resistance of pathogens to conventional antibiotics has created an intensive search for alternative materials with antimicrobial properties. This study is performed with an intention to investigate the combined effects of poly(L-glutamic acid)-capped silver nanoparticles (AgNpPGA) and ascorbic acid (AscH) encapsulated within freeze-dried poly(lactide-co-glycolide) (PLGA) nanospheres to obtain a nanomaterial with simultaneous osteoinductive, antioxidative, and prolonged antimicrobial properties. The influence of PLGA/AgNpPGA/AscH particles on (i) viability and super-oxide production of human umbilical vein endothelial cells in vitro, (ii) morphology and expression of osteogenic markers in osteoblastic MC3T3-E1 cells in vitro, and (iii) antimicrobial activity against a Gram-positive bacterium, methicillin-resistant Staphylococcus aureus, and a Gram-negative bacterium, Escherichia coli, was investigated. PLGA/AgNpPGA/AscH nanoparticles showed a superior and extended antibacterial activity against both types of bacteria. The nanoparticles appeared to be capable of delivering ascorbate to the cells, which was evidenced by the significant decrease in the level of superoxides in human umbilical vein endothelial cells and which could have a therapeutic potential in preventing oxidative stress. PLGA/AgNpPGA/AscH nanoparticles had a positive effect on MC3T3-E1 osteoblastic cells in vitro, promoting: (i) an intimate contact with the cells and preservation of their healthy morphologies; (ii) unreduced cell viability; and (iii) multiple-fold upregulation of two osteogenic markers: osteocalcin and type I procollagen. It is concluded that PLGA/AgNpPGA/AscH nanospheres present a promising new material for the treatment of infections and use in wound dressings and other prophylactic applications.eng
dc.format5 18 (2013) 9034-9042
dc.formatapplication/pdf
dc.languageen
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.relationUnited States National Institutes of Health (NIH) / National Institute of Dental and Craniofacial Research (NIDCR), Grant K99-DE021416
dc.rightsembargoedAccess
dc.sourceACS applied materials and interfaceseng
dc.subjectnanotechnology
dc.subjectPLGA
dc.subjectsilver nanoparticles
dc.subjectosteoinductivity
dc.subjectantibacterial materials
dc.titleComposite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activitieseng
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractШкапин, Сречо Давор; Ускоковић, Драган; Филиповић, Милош; Стевановић, Магдалена; Ускоковић, Вук;
dc.citation.spage9034
dc.citation.epage9042
dc.citation.volume5
dc.citation.issue18
dc.identifier.wos000330016500031
dc.identifier.doi10.1021/am402237g
dc.identifier.scopus2-s2.0-84885049659
dc.description.otherThis is the peer-reviewed version of the articleStevanović, M., Uskoković, V., Filipović, M., Škapin, S.D., Uskoković, D., 2013. Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities. ACS Applied Materials & Interfaces 5, 9034–9042. [https://doi.org/10.1021/am402237g]
dc.type.versionacceptedVersion
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/handle/123456789/15986/10.1021-am402237g.pdf


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record