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dc.creatorIgnjatović, Nenad
dc.creatorPenov Gaši, Katarina
dc.creatorWu, Victoria
dc.creatorAjduković, Jovana
dc.creatorKojić, Vesna V.
dc.creatorVasiljević Radović, Dana
dc.creatorKuzmanović, Maja
dc.creatorUskoković, Vuk
dc.creatorUskoković, Dragan
dc.date.accessioned2018-01-30T10:09:06Z
dc.date.available2018-01-30T10:09:06Z
dc.date.issued2016
dc.identifier.issn0927-7765
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/15974
dc.description.abstractIn an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50=168nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46±2%), while simultaneously preserving high viability (83±3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells.eng
dc.format148 (2016) 629-639
dc.languageen
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172021/RS//
dc.relationUnited States National Institutes of Health, Grant R00-DE021416
dc.rightsrestrictedAccess
dc.sourceColloids and Surfaces B: Biointerfaceseng
dc.subjectandrostane
dc.subjectchitosan
dc.subjectHydroxyapatite
dc.subjectNanoparticle
dc.subjectPLGA
dc.titleSelective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitoreng
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКојић, Весна В.; Васиљевић Радовић, Дана; Кузмановић, Маја; Ускоковић, Вук; Ускоковић, Драган; Игњатовић, Ненад; Пенов-Гаши, Катарина М.; Aјдуковић, Јована Ј.; Wу, Вицториа;
dc.citation.spage629
dc.citation.epage639
dc.citation.volume148
dc.identifier.wos000388248500073
dc.identifier.doi10.1016/j.colsurfb.2016.09.041
dc.identifier.pmid27694053
dc.identifier.scopus2-s2.0-84989184184
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/handle/123456789/15974/Ignjatovic_Colloids-and-Surfaces_B_148_2016_629-639.pdf


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