Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates
Аутори
Ganić, TeaVuletić, Stefana
Stevanović, Magdalena
Kuzmanović, Maja
Cvetković, Stefana
Nikolić, Biljana
Đurović, Saša
Kekić, Dušan
Mitić-Ćulafić, Dragana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective: Acinetobacter baumannii is a pathogenic species which presents a danger to healthcare facilities due to the rapid spread of antibiotic resistance. Plants are currently being explored as a potential source of bioactive compounds that could be used to combat infectious diseases. Cinnamon is used as a food spice, but essential oil has been proven for antimicrobial activity. Due to reduced stability and solubility of essential oil, nanoemulsion (NE) synthesis could provide stronger antimicrobial effects. Investigation and comparison of antimicrobial activity of cinnamon (Cinnamomum zeylanicum L.) bark essential oil (EO) and NE on the A. baumannii ATCC19606 and clinical isolates. Effect of EO and NE on biofilm formation and biofilm eradication.
Methods: GC/MS was performed in order to determine chemical composition of commercially purchased EO (P0125285, Frey + Lau, GmbH, Henstedt-Ulzburg, Germany). Droplet/particle size and polydispersity index of NE was determined by photon co...rrelation spectroscopy (PCS). Minimal inhibitory concentration of EO and NE were defined using MIC assay. Effects of EO and NE were also examined on biofilm formation and eradication. Crystal violet staining was used for biofilm biomass quantification.
Results: GC/MS analysis determined that the most common compound was trans-Cinnamaldehyde (61.9%). NE droplet/particles had multimodal distribution, shown by PCS. MIC values for EO were in range 0.25mg/mL — 0.5mg/mL, and for NE 0.125mg/mL — 0.25mg/mL. Both tested substances showed good effect on biofilm eradication, and destroyed biofilm biomass up to 64%, whilst the inhibition of biofilm formation was up to 70%.
Conclusion: Taking all the results into account, it is a good start for further investigations of both EO and NE as a potential antimicrobial agent. Deeper knowledge about the mechanisms of actions of both of these tested substances could help us to understand the best and the most effective way to use them, in order to combat against Acinetobacter baumannii.
Кључне речи:
Acinetobacter baumannii / antimicrobial activity / cinnamonИзвор:
Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022, 2022, 85-85Издавач:
- Wroclaw : College of Physiotherapy
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Ganić, Tea AU - Vuletić, Stefana AU - Stevanović, Magdalena AU - Kuzmanović, Maja AU - Cvetković, Stefana AU - Nikolić, Biljana AU - Đurović, Saša AU - Kekić, Dušan AU - Mitić-Ćulafić, Dragana PY - 2022 UR - https://dais.sanu.ac.rs/123456789/13610 AB - Objective: Acinetobacter baumannii is a pathogenic species which presents a danger to healthcare facilities due to the rapid spread of antibiotic resistance. Plants are currently being explored as a potential source of bioactive compounds that could be used to combat infectious diseases. Cinnamon is used as a food spice, but essential oil has been proven for antimicrobial activity. Due to reduced stability and solubility of essential oil, nanoemulsion (NE) synthesis could provide stronger antimicrobial effects. Investigation and comparison of antimicrobial activity of cinnamon (Cinnamomum zeylanicum L.) bark essential oil (EO) and NE on the A. baumannii ATCC19606 and clinical isolates. Effect of EO and NE on biofilm formation and biofilm eradication. Methods: GC/MS was performed in order to determine chemical composition of commercially purchased EO (P0125285, Frey + Lau, GmbH, Henstedt-Ulzburg, Germany). Droplet/particle size and polydispersity index of NE was determined by photon correlation spectroscopy (PCS). Minimal inhibitory concentration of EO and NE were defined using MIC assay. Effects of EO and NE were also examined on biofilm formation and eradication. Crystal violet staining was used for biofilm biomass quantification. Results: GC/MS analysis determined that the most common compound was trans-Cinnamaldehyde (61.9%). NE droplet/particles had multimodal distribution, shown by PCS. MIC values for EO were in range 0.25mg/mL — 0.5mg/mL, and for NE 0.125mg/mL — 0.25mg/mL. Both tested substances showed good effect on biofilm eradication, and destroyed biofilm biomass up to 64%, whilst the inhibition of biofilm formation was up to 70%. Conclusion: Taking all the results into account, it is a good start for further investigations of both EO and NE as a potential antimicrobial agent. Deeper knowledge about the mechanisms of actions of both of these tested substances could help us to understand the best and the most effective way to use them, in order to combat against Acinetobacter baumannii. PB - Wroclaw : College of Physiotherapy C3 - Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022 T1 - Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates SP - 85 EP - 85 UR - https://hdl.handle.net/21.15107/rcub_dais_13610 ER -
@conference{ author = "Ganić, Tea and Vuletić, Stefana and Stevanović, Magdalena and Kuzmanović, Maja and Cvetković, Stefana and Nikolić, Biljana and Đurović, Saša and Kekić, Dušan and Mitić-Ćulafić, Dragana", year = "2022", abstract = "Objective: Acinetobacter baumannii is a pathogenic species which presents a danger to healthcare facilities due to the rapid spread of antibiotic resistance. Plants are currently being explored as a potential source of bioactive compounds that could be used to combat infectious diseases. Cinnamon is used as a food spice, but essential oil has been proven for antimicrobial activity. Due to reduced stability and solubility of essential oil, nanoemulsion (NE) synthesis could provide stronger antimicrobial effects. Investigation and comparison of antimicrobial activity of cinnamon (Cinnamomum zeylanicum L.) bark essential oil (EO) and NE on the A. baumannii ATCC19606 and clinical isolates. Effect of EO and NE on biofilm formation and biofilm eradication. Methods: GC/MS was performed in order to determine chemical composition of commercially purchased EO (P0125285, Frey + Lau, GmbH, Henstedt-Ulzburg, Germany). Droplet/particle size and polydispersity index of NE was determined by photon correlation spectroscopy (PCS). Minimal inhibitory concentration of EO and NE were defined using MIC assay. Effects of EO and NE were also examined on biofilm formation and eradication. Crystal violet staining was used for biofilm biomass quantification. Results: GC/MS analysis determined that the most common compound was trans-Cinnamaldehyde (61.9%). NE droplet/particles had multimodal distribution, shown by PCS. MIC values for EO were in range 0.25mg/mL — 0.5mg/mL, and for NE 0.125mg/mL — 0.25mg/mL. Both tested substances showed good effect on biofilm eradication, and destroyed biofilm biomass up to 64%, whilst the inhibition of biofilm formation was up to 70%. Conclusion: Taking all the results into account, it is a good start for further investigations of both EO and NE as a potential antimicrobial agent. Deeper knowledge about the mechanisms of actions of both of these tested substances could help us to understand the best and the most effective way to use them, in order to combat against Acinetobacter baumannii.", publisher = "Wroclaw : College of Physiotherapy", journal = "Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022", title = "Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates", pages = "85-85", url = "https://hdl.handle.net/21.15107/rcub_dais_13610" }
Ganić, T., Vuletić, S., Stevanović, M., Kuzmanović, M., Cvetković, S., Nikolić, B., Đurović, S., Kekić, D.,& Mitić-Ćulafić, D.. (2022). Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates. in Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022 Wroclaw : College of Physiotherapy., 85-85. https://hdl.handle.net/21.15107/rcub_dais_13610
Ganić T, Vuletić S, Stevanović M, Kuzmanović M, Cvetković S, Nikolić B, Đurović S, Kekić D, Mitić-Ćulafić D. Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates. in Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022. 2022;:85-85. https://hdl.handle.net/21.15107/rcub_dais_13610 .
Ganić, Tea, Vuletić, Stefana, Stevanović, Magdalena, Kuzmanović, Maja, Cvetković, Stefana, Nikolić, Biljana, Đurović, Saša, Kekić, Dušan, Mitić-Ćulafić, Dragana, "Cinnamon essential oil and nanoemulsion: antibiofiIm activity on Acinetobacter baumannii clinical isolates" in Programme and the Book of Abstracts / 52nd International Symposium on Essential Oils, Wroclaw, 4-7 September, ISEO 2022 (2022):85-85, https://hdl.handle.net/21.15107/rcub_dais_13610 .