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Regeneracija skeletnih tkiva pomognuta biomaterijalima kao tkivnim matricama - in vivo i in vitro studija

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Regeneracija skeletnih tkiva pomognuta biomaterijalima kao tkivnim matricama - in vivo i in vitro studija (en)
Регенерација скелетних ткива помогнута биоматеријалима као ткивним матрицама - ин виво и ин витро студија (sr)
Regeneracija skeletnih tkiva pomognuta biomaterijalima kao tkivnim matricama - in vivo i in vitro studija (sr_RS)
Authors

Publications

Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold

Vasiljević, Perica; Najman, Stevo; Đorđević, Ljubiša; Savić, Vojin P.; Vukelić, Marija Đ.; Živanov Čurlis, Jelena Z.; Ignjatović, Nenad; Uskoković, Dragan

(Belgrade : Association of the Chemical Engineers of Serbia, 2009)

TY  - JOUR
AU  - Vasiljević, Perica
AU  - Najman, Stevo
AU  - Đorđević, Ljubiša
AU  - Savić, Vojin P.
AU  - Vukelić, Marija Đ.
AU  - Živanov Čurlis, Jelena Z.
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
PY  - 2009
UR  - http://www.ncbi.nlm.nih.gov/pubmed/0367-598X0904301V
UR  - http://dais.sanu.ac.rs/123456789/3362
AB  - Bone tissue reconstruction and reparation is a big challenge in medicine. Biocomposite materials based on hidroxyapatite are widely used in reparation of bone defects. Adult bone marrow-derived stem cells may be considered in two categories: hematopoietic stem cells (HSC) from the bone marrow and mesenchymal stem cells from the bone marrow stroma (BMSC). HSC and BMSC do not only coexist in one organ, but functionally cooperate. BMSC have a critical role in the formation of hematopoietic microenvironment (HME). The aim of this study was to investigate the interactions between bone marrow cells and biocomposites based on HAp/PLLA (hidroxyapatite/poly-L-lactide) after subcutaneous implantation in Balb/c mice. In that purpose, bone marrow cells of Balb/c mice were seeded in HAp/PLLA tubes (15 mm×1,5 mm). The HAp/PLLA tubes with BMC was subcutaneously implanted with a needle into the intrascapsular region of the mouse. Implants were extracted after 2, 6 and 12 weeks. In implants after 2 and 6 weeks we found angiogenesis, collagenogenesis and new bone. Ectopic hematopoiesis was seen in implants after 12 weeks from implantation. As a good scaffold in the role of supporting osteogenesis and hematopoiesis, biocomposites HAp/PLLA can be good bone substitute materials in the bone reparation process. These results showed that the HAp/PLLA scaffold owned biological properties comparable to natural bone.
AB  - Ispitivana je biofunkcionalnost biokompozita hidroksiapatit/poli-L-laktida (HAp/ /PLLA) kao matrice za osteogenezu i hematopoezu posle subkutane implantacije singenim miševima Balb/c. HAp/PLLA oblikovan je u cevčice (15 mm×1,5 mm) koje su napunjene ćelijama koštane srži miša, a zatim implantirane. Kontrole su bile prokuvane tibije i femuri napunjene na isti način. Implanti su ekstrahovani posle 2, 6 i 12 nedelja i analizirani patohistološki. Angiogeneza i osteogeneza se vide već posle dve nedelje na implantima, što je izraženije na kostima. Vrlo izražena hematopoeza u implantima oba tipa se vidi posle dvanaest nedelja. HAp/PLLA može poslužiti kao matrica za ektopičnu osteogenezu i hematopoezu.
PB  - Belgrade : Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold
T1  - Ektopična osteogeneza i hematopoeza iz implantiranih ćelija koštane srži na matrici od biokompozita HAP/PLLA
SP  - 301
EP  - 307
VL  - 63
IS  - 4
DO  - 10.2298/HEMIND0904301V
ER  - 
@article{
author = "Vasiljević, Perica and Najman, Stevo and Đorđević, Ljubiša and Savić, Vojin P. and Vukelić, Marija Đ. and Živanov Čurlis, Jelena Z. and Ignjatović, Nenad and Uskoković, Dragan",
year = "2009",
url = "http://www.ncbi.nlm.nih.gov/pubmed/0367-598X0904301V, http://dais.sanu.ac.rs/123456789/3362",
abstract = "Bone tissue reconstruction and reparation is a big challenge in medicine. Biocomposite materials based on hidroxyapatite are widely used in reparation of bone defects. Adult bone marrow-derived stem cells may be considered in two categories: hematopoietic stem cells (HSC) from the bone marrow and mesenchymal stem cells from the bone marrow stroma (BMSC). HSC and BMSC do not only coexist in one organ, but functionally cooperate. BMSC have a critical role in the formation of hematopoietic microenvironment (HME). The aim of this study was to investigate the interactions between bone marrow cells and biocomposites based on HAp/PLLA (hidroxyapatite/poly-L-lactide) after subcutaneous implantation in Balb/c mice. In that purpose, bone marrow cells of Balb/c mice were seeded in HAp/PLLA tubes (15 mm×1,5 mm). The HAp/PLLA tubes with BMC was subcutaneously implanted with a needle into the intrascapsular region of the mouse. Implants were extracted after 2, 6 and 12 weeks. In implants after 2 and 6 weeks we found angiogenesis, collagenogenesis and new bone. Ectopic hematopoiesis was seen in implants after 12 weeks from implantation. As a good scaffold in the role of supporting osteogenesis and hematopoiesis, biocomposites HAp/PLLA can be good bone substitute materials in the bone reparation process. These results showed that the HAp/PLLA scaffold owned biological properties comparable to natural bone., Ispitivana je biofunkcionalnost biokompozita hidroksiapatit/poli-L-laktida (HAp/ /PLLA) kao matrice za osteogenezu i hematopoezu posle subkutane implantacije singenim miševima Balb/c. HAp/PLLA oblikovan je u cevčice (15 mm×1,5 mm) koje su napunjene ćelijama koštane srži miša, a zatim implantirane. Kontrole su bile prokuvane tibije i femuri napunjene na isti način. Implanti su ekstrahovani posle 2, 6 i 12 nedelja i analizirani patohistološki. Angiogeneza i osteogeneza se vide već posle dve nedelje na implantima, što je izraženije na kostima. Vrlo izražena hematopoeza u implantima oba tipa se vidi posle dvanaest nedelja. HAp/PLLA može poslužiti kao matrica za ektopičnu osteogenezu i hematopoezu.",
publisher = "Belgrade : Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Ectopic osteogenesis and hematopoiesis after implantantion of bone marrow cells seeded on HAP/PLLA scaffold, Ektopična osteogeneza i hematopoeza iz implantiranih ćelija koštane srži na matrici od biokompozita HAP/PLLA",
pages = "301-307",
volume = "63",
number = "4",
doi = "10.2298/HEMIND0904301V"
}
3
1
3

Nanomaterial N-CP/DLPLG as potentional tissue graft in osteoreparation in combination with bone marrow cells on subcutaneous implantation model

Janićijević, Jelena M.; Najman, Stevo; Ignjatović, Nenad; Savić, Vojin P.; Kocić, Jelena S.; Vasiljević, Perica; Vukelić, Marija Đ.; Uskoković, Dragan

(Belgrade : Association of the Chemical Engineers of Serbia, 2008)

TY  - JOUR
AU  - Janićijević, Jelena M.
AU  - Najman, Stevo
AU  - Ignjatović, Nenad
AU  - Savić, Vojin P.
AU  - Kocić, Jelena S.
AU  - Vasiljević, Perica
AU  - Vukelić, Marija Đ.
AU  - Uskoković, Dragan
PY  - 2008
UR  - http://www.ncbi.nlm.nih.gov/pubmed/0367-598X0803205J
UR  - http://dais.sanu.ac.rs/123456789/3363
AB  - The need for bone graft materials in osteoreparation is tremendous. Many researches have shown that calcium-phosphate bioceramics have good biocompatibility and osteoconductivity. We used nanocomposite biomaterial calcium phosphate coated with poly (dl-lactide-co-glycolide) or N-CP/DLPLG. The goal of this investigation was to examine weather N-CP/DLPLG has ability to sustain growth of bone marrow cells after subcutaneous implantation in Balb/c mice. For that purpose N-CP/DLPLG implants with and without bone marrow cells (control) were made. Implants were extracted after eight days and eight weeks. In implants loaded with bone marrow cells after eight days and eight weeks we observed fields rich in cells, angiogenesis and collagen genesis. These results showed that N-CP/DLPLG has property of tissue scaffold which sustain bone marrow cells growth and collagen production. This represents a good way for further examination of N-CP/DLPLG as potentional tissue scaffold in osteoreparation.
PB  - Belgrade : Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T2  - Hemijska industrija
T1  - Nanomaterial N-CP/DLPLG as potentional tissue graft in osteoreparation in combination with bone marrow cells on subcutaneous implantation model
SP  - 205
EP  - 210
VL  - 62
IS  - 3
DO  - 10.2298/HEMIND0803205J
ER  - 
@article{
author = "Janićijević, Jelena M. and Najman, Stevo and Ignjatović, Nenad and Savić, Vojin P. and Kocić, Jelena S. and Vasiljević, Perica and Vukelić, Marija Đ. and Uskoković, Dragan",
year = "2008",
url = "http://www.ncbi.nlm.nih.gov/pubmed/0367-598X0803205J, http://dais.sanu.ac.rs/123456789/3363",
abstract = "The need for bone graft materials in osteoreparation is tremendous. Many researches have shown that calcium-phosphate bioceramics have good biocompatibility and osteoconductivity. We used nanocomposite biomaterial calcium phosphate coated with poly (dl-lactide-co-glycolide) or N-CP/DLPLG. The goal of this investigation was to examine weather N-CP/DLPLG has ability to sustain growth of bone marrow cells after subcutaneous implantation in Balb/c mice. For that purpose N-CP/DLPLG implants with and without bone marrow cells (control) were made. Implants were extracted after eight days and eight weeks. In implants loaded with bone marrow cells after eight days and eight weeks we observed fields rich in cells, angiogenesis and collagen genesis. These results showed that N-CP/DLPLG has property of tissue scaffold which sustain bone marrow cells growth and collagen production. This represents a good way for further examination of N-CP/DLPLG as potentional tissue scaffold in osteoreparation.",
publisher = "Belgrade : Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija, Hemijska industrija",
title = "Nanomaterial N-CP/DLPLG as potentional tissue graft in osteoreparation in combination with bone marrow cells on subcutaneous implantation model",
pages = "205-210",
volume = "62",
number = "3",
doi = "10.2298/HEMIND0803205J"
}
5
2
3

Osteogenic activity in a mice subcutaneous implant of porous hydroxyapatite/poly-L-lactide loaded with bone marrow cells

Janićijević, Jelena; Najman, Stevo; Vukelić, Marija; Ignjatović, Nenad; Uskoković, Dragan; Đorđević, Ljubiša; Vasiljević, Perica

(Belgrade : Institute of Technical Sciences of SASA, 2007)

TY  - CONF
AU  - Janićijević, Jelena
AU  - Najman, Stevo
AU  - Vukelić, Marija
AU  - Ignjatović, Nenad
AU  - Uskoković, Dragan
AU  - Đorđević, Ljubiša
AU  - Vasiljević, Perica
PY  - 2007
UR  - 181
UR  - http://dais.sanu.ac.rs/123456789/6283
AB  - Hydroxyapatite (HAp) is biomaterial widely used in the regeneration of bone tissue.
Addition of osteogenic cells to HAp implants may accelerate the bone repair process. The aim of
this study was to investigate how the bone marrow cells (BMCs) loading of porous
hydroxyapatite/poly-L-lactide (HAp/PLLA) act to ectopic osteogenesis. In this purpose
HAp/PLLA with and without BMCs was subcutaneously implanted into BALB/c mice. As a
control served implants from both types which weren’t implanted. Three weeks after
implantation, histological analysis of implants was done. It was observed significant resorption
and induction of collagenogenesis in implanted biomaterials. The structure of new bone was
seen in implants loaded with bone marrow cells.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Programme and The Book of Abstracts / Ninth Annual Conference YUCOMAT 2007, Herceg Novi, September 10-14, 2007
T1  - Osteogenic activity in a mice subcutaneous implant of porous hydroxyapatite/poly-L-lactide loaded with bone marrow cells
SP  - 181
EP  - 181
ER  - 
@conference{
author = "Janićijević, Jelena and Najman, Stevo and Vukelić, Marija and Ignjatović, Nenad and Uskoković, Dragan and Đorđević, Ljubiša and Vasiljević, Perica",
year = "2007",
url = "181, http://dais.sanu.ac.rs/123456789/6283",
abstract = "Hydroxyapatite (HAp) is biomaterial widely used in the regeneration of bone tissue.
Addition of osteogenic cells to HAp implants may accelerate the bone repair process. The aim of
this study was to investigate how the bone marrow cells (BMCs) loading of porous
hydroxyapatite/poly-L-lactide (HAp/PLLA) act to ectopic osteogenesis. In this purpose
HAp/PLLA with and without BMCs was subcutaneously implanted into BALB/c mice. As a
control served implants from both types which weren’t implanted. Three weeks after
implantation, histological analysis of implants was done. It was observed significant resorption
and induction of collagenogenesis in implanted biomaterials. The structure of new bone was
seen in implants loaded with bone marrow cells.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Programme and The Book of Abstracts / Ninth Annual Conference YUCOMAT 2007, Herceg Novi, September 10-14, 2007",
title = "Osteogenic activity in a mice subcutaneous implant of porous hydroxyapatite/poly-L-lactide loaded with bone marrow cells",
pages = "181-181"
}

Nanomaterijal N-CP/PLGLP kao potencijalna tkivna matrica u osteoreparaciji pomoću kostne srži na modelu subkutane implantacije

Janićijević, Jelena; Najman, Stevo; Ignjatović, Nenad; Savić, Vojin; Kocić, Jelena; Vasiljević, Perica; Uskoković, Dragan

(Beograd : Institut tehničkih nauka SANU, 2007)

TY  - CONF
AU  - Janićijević, Jelena
AU  - Najman, Stevo
AU  - Ignjatović, Nenad
AU  - Savić, Vojin
AU  - Kocić, Jelena
AU  - Vasiljević, Perica
AU  - Uskoković, Dragan
PY  - 2007
UR  - http://dais.sanu.ac.rs/123456789/6273
AB  - Ispitivan je nanomaterijal N-CP/PLGLP kao potencijalna tkivna matrica za reparaciju kosti pri korišćenju kostne srži kao izvora osteogenih ćelija na modelu subkutane implantacije singenim miševima Balb/c. Implanti su napravljeni od nanomaterijala mešanjem sa razblaženom krvlju i kostnom srži, a ekstrahovani su nakon osam dana i osam nedelja. Implanti bez kostne srži su bili kontrola. Nakon osam dana se na periferiji implanta vide gusta polja sa ćelijama, a u materijalu se vidi lakunarna organizacija sa prisustvom ćelija. Uočljiva je vaskularizacija i registrovan je kolagen. Na kontrolnim implantima je celularnost vrlo slaba. Posle osam nedelja redukovana je površina nanomaterijala i celularnost implanata.
PB  - Beograd : Institut tehničkih nauka SANU
C3  - Program i knjiga apstrakata / Šesta konferencija mladih istraživača Nauka i inženjerstvo novih materijala, Beograd, 24–26. decembar 2007
T1  - Nanomaterijal N-CP/PLGLP kao potencijalna tkivna matrica u osteoreparaciji pomoću kostne srži na modelu subkutane implantacije
SP  - 5
EP  - 5
ER  - 
@conference{
author = "Janićijević, Jelena and Najman, Stevo and Ignjatović, Nenad and Savić, Vojin and Kocić, Jelena and Vasiljević, Perica and Uskoković, Dragan",
year = "2007",
url = "http://dais.sanu.ac.rs/123456789/6273",
abstract = "Ispitivan je nanomaterijal N-CP/PLGLP kao potencijalna tkivna matrica za reparaciju kosti pri korišćenju kostne srži kao izvora osteogenih ćelija na modelu subkutane implantacije singenim miševima Balb/c. Implanti su napravljeni od nanomaterijala mešanjem sa razblaženom krvlju i kostnom srži, a ekstrahovani su nakon osam dana i osam nedelja. Implanti bez kostne srži su bili kontrola. Nakon osam dana se na periferiji implanta vide gusta polja sa ćelijama, a u materijalu se vidi lakunarna organizacija sa prisustvom ćelija. Uočljiva je vaskularizacija i registrovan je kolagen. Na kontrolnim implantima je celularnost vrlo slaba. Posle osam nedelja redukovana je površina nanomaterijala i celularnost implanata.",
publisher = "Beograd : Institut tehničkih nauka SANU",
journal = "Program i knjiga apstrakata / Šesta konferencija mladih istraživača Nauka i inženjerstvo novih materijala, Beograd, 24–26. decembar 2007",
title = "Nanomaterijal N-CP/PLGLP kao potencijalna tkivna matrica u osteoreparaciji pomoću kostne srži na modelu subkutane implantacije",
pages = "5-5"
}