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Cundrič, Sandra

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  • Cundrič, Sandra (5)
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Author's Bibliography

Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells

Filipović, Nenad; Stevanović, Magdalena; Nunić, Jana; Cundrič, Sandra; Filipič, Metka; Uskoković, Dragan

(Elsevier, 2014)

TY  - JOUR
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/367
AB  - Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.
PB  - Elsevier
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells
SP  - 414
EP  - 424
VL  - 117
IS  - 1
DO  - 10.1016/j.colsurfb.2014.03.015
ER  - 
@article{
author = "Filipović, Nenad and Stevanović, Magdalena and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/367",
abstract = "Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.",
publisher = "Elsevier",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells",
pages = "414-424",
volume = "117",
number = "1",
doi = "10.1016/j.colsurfb.2014.03.015"
}
Filipović, N., Stevanović, M., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D. (2014). Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells.
Colloids and Surfaces B: BiointerfacesElsevier., 117(1), 414-424. 
https://doi.org/10.1016/j.colsurfb.2014.03.015
Filipović N, Stevanović M, Nunić J, Cundrič S, Filipič M, Uskoković D. Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. Colloids and Surfaces B: Biointerfaces. 2014;117(1):414-424
8
9
11

Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells

Filipović, Nenad; Stevanović, Magdalena; Nunić, Jana; Cundrič, Sandra; Filipič, Metka; Uskoković, Dragan

(Elsevier, 2014)

TY  - JOUR
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/330
AB  - Nanospheres of poly (ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.
PB  - Elsevier
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells
SP  - 414
EP  - 424
VL  - 117
IS  - 1
DO  - 10.1016/j.colsurfb.2014.03.015
ER  - 
@article{
author = "Filipović, Nenad and Stevanović, Magdalena and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/330",
abstract = "Nanospheres of poly (ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.",
publisher = "Elsevier",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells",
pages = "414-424",
volume = "117",
number = "1",
doi = "10.1016/j.colsurfb.2014.03.015"
}
Filipović, N., Stevanović, M., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D. (2014). Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells.
Colloids and Surfaces B: BiointerfacesElsevier., 117(1), 414-424. 
https://doi.org/10.1016/j.colsurfb.2014.03.015
Filipović N, Stevanović M, Nunić J, Cundrič S, Filipič M, Uskoković D. Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. Colloids and Surfaces B: Biointerfaces. 2014;117(1):414-424
8
9
10

Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species

Stupar, Petar; Pavlović, Vladimir B.; Nunić, Jana; Cundrič, Sandra; Filipič, Metka; Stevanović, Magdalena

(Paris : Association de pharmacie galenique industrielle, 2014)

TY  - JOUR
AU  - Stupar, Petar
AU  - Pavlović, Vladimir B.
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/425
AB  - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
PB  - Paris : Association de pharmacie galenique industrielle
T2  - Journal of Drug Delivery Science and Technology
T1  - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
SP  - 191
EP  - 197
VL  - 24
IS  - 2
DO  - 10.1016/S1773-2247(14)50031-7
ER  - 
@article{
author = "Stupar, Petar and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Stevanović, Magdalena",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/425",
abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.",
publisher = "Paris : Association de pharmacie galenique industrielle",
journal = "Journal of Drug Delivery Science and Technology",
title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species",
pages = "191-197",
volume = "24",
number = "2",
doi = "10.1016/S1773-2247(14)50031-7"
}
Stupar, P., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Stevanović, M. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species.
Journal of Drug Delivery Science and TechnologyParis : Association de pharmacie galenique industrielle., 24(2), 191-197. 
https://doi.org/10.1016/S1773-2247(14)50031-7
Stupar P, Pavlović VB, Nunić J, Cundrič S, Filipič M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. Journal of Drug Delivery Science and Technology. 2014;24(2):191-197
4
3
4

Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species

Stupar, Petar; Pavlović, Vladimir B.; Nunić, Jana; Cundrič, Sandra; Filipič, Metka; Stevanović, Magdalena

(Paris : Association de pharmacie galenique industrielle, 2014)

TY  - JOUR
AU  - Stupar, Petar
AU  - Pavlović, Vladimir B.
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/4671
AB  - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
PB  - Paris : Association de pharmacie galenique industrielle
T2  - Journal of Drug Delivery Science and Technology
T1  - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
SP  - 191
EP  - 197
VL  - 24
IS  - 2
DO  - 10.1016/S1773-2247(14)50031-7
ER  - 
@article{
author = "Stupar, Petar and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Stevanović, Magdalena",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/4671",
abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.",
publisher = "Paris : Association de pharmacie galenique industrielle",
journal = "Journal of Drug Delivery Science and Technology",
title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species",
pages = "191-197",
volume = "24",
number = "2",
doi = "10.1016/S1773-2247(14)50031-7"
}
Stupar, P., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Stevanović, M. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species.
Journal of Drug Delivery Science and TechnologyParis : Association de pharmacie galenique industrielle., 24(2), 191-197. 
https://doi.org/10.1016/S1773-2247(14)50031-7
Stupar P, Pavlović VB, Nunić J, Cundrič S, Filipič M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. Journal of Drug Delivery Science and Technology. 2014;24(2):191-197
4
3
4

Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres

Stupar, Petar; Stevanović, Magdalena; Filipović, Nenad; Pavlović, Vladimir B.; Nunić, Jana; Cundrič, Sandra; Filipič, Metka; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade, 2012)

TY  - CONF
AU  - Stupar, Petar
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Pavlović, Vladimir B.
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/531
AB  - A common limitation of using polymeric micro and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. The aim of this study was to evaluate different cryoprotectants (protective excipients that are usually added to increase stability upon storage and protect the particles from freezing stress): sugars (glucose and sucrose) and polymers (PVA and PGA), on the outcome of freeze-dried poly(ε-caprolactone) micro and nanospheres. The best freeze-drying results in terms of morphological characteristics, analyzed with SEM, were achieved with glucose at concentration of 1%. The FTIR analysis confirmed that the molecular structure of PCL particles remained the same after the addition cryoprotectants.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres
SP  - 104
EP  - 104
ER  - 
@conference{
author = "Stupar, Petar and Stevanović, Magdalena and Filipović, Nenad and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/531",
abstract = "A common limitation of using polymeric micro and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. The aim of this study was to evaluate different cryoprotectants (protective excipients that are usually added to increase stability upon storage and protect the particles from freezing stress): sugars (glucose and sucrose) and polymers (PVA and PGA), on the outcome of freeze-dried poly(ε-caprolactone) micro and nanospheres. The best freeze-drying results in terms of morphological characteristics, analyzed with SEM, were achieved with glucose at concentration of 1%. The FTIR analysis confirmed that the molecular structure of PCL particles remained the same after the addition cryoprotectants.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres",
pages = "104-104"
}
Stupar, P., Stevanović, M., Filipović, N., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D. (2012). Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres.
Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of AbstractsBelgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., null, 104-104. 
Stupar P, Stevanović M, Filipović N, Pavlović VB, Nunić J, Cundrič S, Filipič M, Uskoković D. Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres. Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:104-104