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Petković, Jana

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  • Petković, Jana (7)
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Author's Bibliography

Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential

Stevanović, Magdalena; Škapin, Srečo Davor; Bračko, Ines; Milenković, Marina; Petković, Jana; Filipič, Metka; Uskoković, Dragan

(Elsevier, 2012)

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Škapin, Srečo Davor
AU  - Bračko, Ines
AU  - Milenković, Marina
AU  - Petković, Jana
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/488
AB  - Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (α, γ, l-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV–VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.
PB  - Elsevier
T2  - Polymer
T1  - Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential
SP  - 2818
EP  - 2828
VL  - 53
IS  - 14
DO  - 10.1016/j.polymer.2012.04.057
ER  - 
@article{
author = "Stevanović, Magdalena and Škapin, Srečo Davor and Bračko, Ines and Milenković, Marina and Petković, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/488",
abstract = "Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (α, γ, l-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV–VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.",
publisher = "Elsevier",
journal = "Polymer",
title = "Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential",
pages = "2818-2828",
volume = "53",
number = "14",
doi = "10.1016/j.polymer.2012.04.057"
}
Stevanović, M., Škapin, S. D., Bračko, I., Milenković, M., Petković, J., Filipič, M.,& Uskoković, D. (2012). Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential.
PolymerElsevier., 53(14), 2818-2828. 
https://doi.org/10.1016/j.polymer.2012.04.057
Stevanović M, Škapin SD, Bračko I, Milenković M, Petković J, Filipič M, Uskoković D. Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential. Polymer. 2012;53(14):2818-2828
54
53
55

Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide)

Stevanović, Magdalena; Milenković, Marina; Petković, Jana; Filipič, Metka; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2012)

TY  - CONF
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
AU  - Petković, Jana
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/457
AB  - Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (Lglutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over the long periods of time without signs of precipitation. Ascorbic acid, a water soluble antioxidant, was encapsulated together with these stable AgNpPGAs within poly(DL-lactide-coglycolide) polymeric matrix and their synergistic antimicrobial effect was studied. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of the samples. To establish the influence of PLGA/AgNpPGA/ascorbic acid nanoparticles on intracellular ROS formation, we measured the kinetics of their formation in HepG2 cells by DCFH-DA assay. The samples were characterized by UV-VIS spectrometry, field-emission scanning electron microscopy, and transmission electron microscopy.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide)
SP  - 124
EP  - 124
ER  - 
@conference{
author = "Stevanović, Magdalena and Milenković, Marina and Petković, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/457",
abstract = "Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (Lglutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over the long periods of time without signs of precipitation. Ascorbic acid, a water soluble antioxidant, was encapsulated together with these stable AgNpPGAs within poly(DL-lactide-coglycolide) polymeric matrix and their synergistic antimicrobial effect was studied. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of the samples. To establish the influence of PLGA/AgNpPGA/ascorbic acid nanoparticles on intracellular ROS formation, we measured the kinetics of their formation in HepG2 cells by DCFH-DA assay. The samples were characterized by UV-VIS spectrometry, field-emission scanning electron microscopy, and transmission electron microscopy.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide)",
pages = "124-124"
}
Stevanović, M., Milenković, M., Petković, J., Filipič, M.,& Uskoković, D. (2012). Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide).
The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of AbstractsBelgrade : Materials Research Society of Serbia., null, 124-124. 
Stevanović M, Milenković M, Petković J, Filipič M, Uskoković D. Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide). The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:124-124

Freeze-drying method to produce a range of PCL particles with tailored morphological properties

Filipović, Nenad; Stevanović, Magdalena; Stupar, Petar; Petković, Jana; Filipič, Metka; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2012)

TY  - CONF
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Stupar, Petar
AU  - Petković, Jana
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/456
AB  - Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Freeze-drying method to produce a range of PCL particles with tailored morphological properties
SP  - 124
EP  - 124
ER  - 
@conference{
author = "Filipović, Nenad and Stevanović, Magdalena and Stupar, Petar and Petković, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/456",
abstract = "Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Freeze-drying method to produce a range of PCL particles with tailored morphological properties",
pages = "124-124"
}
Filipović, N., Stevanović, M., Stupar, P., Petković, J., Filipič, M.,& Uskoković, D. (2012). Freeze-drying method to produce a range of PCL particles with tailored morphological properties.
The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of AbstractsBelgrade : Materials Research Society of Serbia., null, 124-124. 
Filipović N, Stevanović M, Stupar P, Petković J, Filipič M, Uskoković D. Freeze-drying method to produce a range of PCL particles with tailored morphological properties. The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:124-124

DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells

Petković, Jana; Žegura, Bojana; Stevanović, Magdalena; Drnovšek, Nataša; Uskoković, Dragan; Novak, Saša; Filipič, Metka

(Oxon : Taylor & Francis, 2011)

TY  - JOUR
AU  - Petković, Jana
AU  - Žegura, Bojana
AU  - Stevanović, Magdalena
AU  - Drnovšek, Nataša
AU  - Uskoković, Dragan
AU  - Novak, Saša
AU  - Filipič, Metka
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/568
AB  - We investigated the genotoxic responses to two types of TiO2 nanoparticles (<25 nm anatase: TiO2-An, and <100 nm rutile: TiO2-Ru) in human hepatoma HepG2 cells. Under the applied exposure conditions the particles were agglomerated or aggregated with the size of agglomerates and aggregates in the micrometer range, and were not cytotoxic. TiO2-An, but not TiO2-Ru, caused a persistent increase in DNA strand breaks (comet assay) and oxidized purines (Fpg-comet). TiO2-An was a stronger inducer of intracellular reactive oxygen species (ROS) than TiO2-Ru. Both types of TiO2 nanoparticles transiently upregulated mRNA expression of p53 and its downstream regulated DNA damage responsive genes (mdm2, gadd45α, p21), providing additional evidence that TiO2 nanoparticles are genotoxic. The observed differences in responses of HepG2 cells to exposure to anatase and rutile TiO2 nanoparticles support the evidence that the toxic potential of TiO2 nanoparticles varies not only with particle size but also with crystalline structure.
PB  - Oxon : Taylor & Francis
T2  - Nanotoxicology
T1  - DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells
SP  - 341
EP  - 353
VL  - 5
IS  - 3
DO  - 10.3109/17435390.2010.507316
ER  - 
@article{
author = "Petković, Jana and Žegura, Bojana and Stevanović, Magdalena and Drnovšek, Nataša and Uskoković, Dragan and Novak, Saša and Filipič, Metka",
year = "2011",
url = "http://dais.sanu.ac.rs/123456789/568",
abstract = "We investigated the genotoxic responses to two types of TiO2 nanoparticles (<25 nm anatase: TiO2-An, and <100 nm rutile: TiO2-Ru) in human hepatoma HepG2 cells. Under the applied exposure conditions the particles were agglomerated or aggregated with the size of agglomerates and aggregates in the micrometer range, and were not cytotoxic. TiO2-An, but not TiO2-Ru, caused a persistent increase in DNA strand breaks (comet assay) and oxidized purines (Fpg-comet). TiO2-An was a stronger inducer of intracellular reactive oxygen species (ROS) than TiO2-Ru. Both types of TiO2 nanoparticles transiently upregulated mRNA expression of p53 and its downstream regulated DNA damage responsive genes (mdm2, gadd45α, p21), providing additional evidence that TiO2 nanoparticles are genotoxic. The observed differences in responses of HepG2 cells to exposure to anatase and rutile TiO2 nanoparticles support the evidence that the toxic potential of TiO2 nanoparticles varies not only with particle size but also with crystalline structure.",
publisher = "Oxon : Taylor & Francis",
journal = "Nanotoxicology",
title = "DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells",
pages = "341-353",
volume = "5",
number = "3",
doi = "10.3109/17435390.2010.507316"
}
Petković, J., Žegura, B., Stevanović, M., Drnovšek, N., Uskoković, D., Novak, S.,& Filipič, M. (2011). DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells.
NanotoxicologyOxon : Taylor & Francis., 5(3), 341-353. 
https://doi.org/10.3109/17435390.2010.507316
Petković J, Žegura B, Stevanović M, Drnovšek N, Uskoković D, Novak S, Filipič M. DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells. Nanotoxicology. 2011;5(3):341-353
159
147
154

Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles

Stevanović, Magdalena; Kovačević, Branimir; Petković, Jana; Filipič, Metka; Uskoković, Dragan

(Dove Medical Press, 2011)

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Kovačević, Branimir
AU  - Petković, Jana
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/900
AB  - Highly stable dispersions of nanosized silver particles were synthesized using a straightforward, cost-effective, and ecofriendly method. Nontoxic glucose was utilized as a reducing agent and poly- α, γ, L-glutamic acid (PGA), a naturally occurring anionic polymer, was used as a capping agent to protect the silver nanoparticles from agglomeration and render them biocompatible. Use of ammonia during synthesis was avoided. Our study clearly demonstrates how the concentration of the capping agent plays a major role in determining the dimensions, morphology, and stability, as well as toxicity of a silver colloidal solution. Hence, proper optimization is necessary to develop silver colloids of narrow size distribution. The samples were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurement. MTT assay results indicated good biocompatibility of the PGA-capped silver nanoparticles. Formation of intracellular reactive oxygen species was measured spectrophotometrically using 2,7-dichlorofluorescein diacetate as a fluorescent probe, and it was shown that the PGA-capped silver nanoparticles did not induce intracellular formation of reactive oxygen species.
PB  - Dove Medical Press
T2  - International Journal of Nanomedicine
T1  - Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
SP  - 2837
EP  - 2837
DO  - 10.2147/IJN.S24889
ER  - 
@article{
author = "Stevanović, Magdalena and Kovačević, Branimir and Petković, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2011",
url = "http://dais.sanu.ac.rs/123456789/900",
abstract = "Highly stable dispersions of nanosized silver particles were synthesized using a straightforward, cost-effective, and ecofriendly method. Nontoxic glucose was utilized as a reducing agent and poly- α, γ, L-glutamic acid (PGA), a naturally occurring anionic polymer, was used as a capping agent to protect the silver nanoparticles from agglomeration and render them biocompatible. Use of ammonia during synthesis was avoided. Our study clearly demonstrates how the concentration of the capping agent plays a major role in determining the dimensions, morphology, and stability, as well as toxicity of a silver colloidal solution. Hence, proper optimization is necessary to develop silver colloids of narrow size distribution. The samples were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurement. MTT assay results indicated good biocompatibility of the PGA-capped silver nanoparticles. Formation of intracellular reactive oxygen species was measured spectrophotometrically using 2,7-dichlorofluorescein diacetate as a fluorescent probe, and it was shown that the PGA-capped silver nanoparticles did not induce intracellular formation of reactive oxygen species.",
publisher = "Dove Medical Press",
journal = "International Journal of Nanomedicine",
title = "Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles",
pages = "2837-2837",
doi = "10.2147/IJN.S24889"
}
Stevanović, M., Kovačević, B., Petković, J., Filipič, M.,& Uskoković, D. (2011). Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles.
International Journal of NanomedicineDove Medical Press., null, 2837-2837. 
https://doi.org/10.2147/IJN.S24889
Stevanović M, Kovačević B, Petković J, Filipič M, Uskoković D. Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles. International Journal of Nanomedicine. 2011;:2837-2837
32
32
34

DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells

Petković, Jana; Žegura, Bojana; Stevanović, Magdalena; Drnovšek, Nataša; Uskoković, Dragan; Novak, Saša; Filipič, Metka

(Oxon : Taylor & Francis, 2011)

TY  - JOUR
AU  - Petković, Jana
AU  - Žegura, Bojana
AU  - Stevanović, Magdalena
AU  - Drnovšek, Nataša
AU  - Uskoković, Dragan
AU  - Novak, Saša
AU  - Filipič, Metka
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/4672
AB  - We investigated the genotoxic responses to two types of TiO2 nanoparticles (<25 nm anatase: TiO2-An, and <100 nm rutile: TiO2-Ru) in human hepatoma HepG2 cells. Under the applied exposure conditions the particles were agglomerated or aggregated with the size of agglomerates and aggregates in the micrometer range, and were not cytotoxic. TiO2-An, but not TiO2-Ru, caused a persistent increase in DNA strand breaks (comet assay) and oxidized purines (Fpg-comet). TiO2-An was a stronger inducer of intracellular reactive oxygen species (ROS) than TiO2-Ru. Both types of TiO2 nanoparticles transiently upregulated mRNA expression of p53 and its downstream regulated DNA damage responsive genes (mdm2, gadd45α, p21), providing additional evidence that TiO2 nanoparticles are genotoxic. The observed differences in responses of HepG2 cells to exposure to anatase and rutile TiO2 nanoparticles support the evidence that the toxic potential of TiO2 nanoparticles varies not only with particle size but also with crystalline structure.
PB  - Oxon : Taylor & Francis
T2  - Nanotoxicology
T1  - DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells
SP  - 341
EP  - 353
VL  - 5
IS  - 3
DO  - 10.3109/17435390.2010.507316
ER  - 
@article{
author = "Petković, Jana and Žegura, Bojana and Stevanović, Magdalena and Drnovšek, Nataša and Uskoković, Dragan and Novak, Saša and Filipič, Metka",
year = "2011",
url = "http://dais.sanu.ac.rs/123456789/4672",
abstract = "We investigated the genotoxic responses to two types of TiO2 nanoparticles (<25 nm anatase: TiO2-An, and <100 nm rutile: TiO2-Ru) in human hepatoma HepG2 cells. Under the applied exposure conditions the particles were agglomerated or aggregated with the size of agglomerates and aggregates in the micrometer range, and were not cytotoxic. TiO2-An, but not TiO2-Ru, caused a persistent increase in DNA strand breaks (comet assay) and oxidized purines (Fpg-comet). TiO2-An was a stronger inducer of intracellular reactive oxygen species (ROS) than TiO2-Ru. Both types of TiO2 nanoparticles transiently upregulated mRNA expression of p53 and its downstream regulated DNA damage responsive genes (mdm2, gadd45α, p21), providing additional evidence that TiO2 nanoparticles are genotoxic. The observed differences in responses of HepG2 cells to exposure to anatase and rutile TiO2 nanoparticles support the evidence that the toxic potential of TiO2 nanoparticles varies not only with particle size but also with crystalline structure.",
publisher = "Oxon : Taylor & Francis",
journal = "Nanotoxicology",
title = "DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells",
pages = "341-353",
volume = "5",
number = "3",
doi = "10.3109/17435390.2010.507316"
}
Petković, J., Žegura, B., Stevanović, M., Drnovšek, N., Uskoković, D., Novak, S.,& Filipič, M. (2011). DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells.
NanotoxicologyOxon : Taylor & Francis., 5(3), 341-353. 
https://doi.org/10.3109/17435390.2010.507316
Petković J, Žegura B, Stevanović M, Drnovšek N, Uskoković D, Novak S, Filipič M. DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells. Nanotoxicology. 2011;5(3):341-353
159
147
154

An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres

Stevanović, Magdalena; Maksin, Tatjana; Petković, Jana; Filipič, Metka; Uskoković, Dragan

(Bristol : IOP Science, 2009)

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Maksin, Tatjana
AU  - Petković, Jana
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2009
UR  - http://dais.sanu.ac.rs/123456789/2740
AB  - Nanoparticles of poly(DL-lactide-co-glycolide) (PLGA) in the size range 90-150 nm were produced using the physicochemical method with solvent/non-solvent systems. The encapsulation of the ascorbic acid in the polymer matrix was performed by homogenization of the water and organic phases. In vitro degradation and release tests of PLGA nanoparticles with and without encapsulated ascorbic acid were studied for more than 60 days in PBS and it has been determined that PLGA completely degrades within this period, fully releasing all encapsulated ascorbic acid. The cytotoxicity of PLGA and PLGA/ascorbic acid 85/15% nanoparticles was examined with human hepatoma cell lines (HepG2 ECACC), in vitro. The obtained results indicate that neither PLGA nanospheres nor PLGA/ascorbic acid 85/15% nanoparticles significantly affected the viability of the HepG2 cells. The investigation of the distribution and pharmacokinetics of PLGA is crucial for the effective prediction of host responses to PLGA in particular applications. Thus we present a method of labeling PLGA nanospheres and PLGA/ascorbic acid 85/15 wt% nanoparticles by (99m)Tc which binds outside, leaving the cage intact. This enables a quick and convenient investigation of the pharmacological behavior and metabolism of PLGA. The biodistribution of (99m)Tc-labeled PLGA particles with and without encapsulated ascorbic acid after different periods of time of their installation into rats was examined. PLGA nanospheres with encapsulated ascorbic acid exhibit prolonged blood circulation accompanied by time-dependent reduction in the lungs, liver and spleen, and addition in the kidney, stomach and intestine. The samples were characterized by x-ray diffraction, scanning electron microscopy, stereological analysis, transmission electron microscopy, ultraviolet spectroscopy and instant thin layer chromatography.
PB  - Bristol : IOP Science
T2  - Nanotechnology
T1  - An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres
VL  - 20
IS  - 33
DO  - 10.1088/0957-4484/20/33/335102
ER  - 
@article{
author = "Stevanović, Magdalena and Maksin, Tatjana and Petković, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2009",
url = "http://dais.sanu.ac.rs/123456789/2740",
abstract = "Nanoparticles of poly(DL-lactide-co-glycolide) (PLGA) in the size range 90-150 nm were produced using the physicochemical method with solvent/non-solvent systems. The encapsulation of the ascorbic acid in the polymer matrix was performed by homogenization of the water and organic phases. In vitro degradation and release tests of PLGA nanoparticles with and without encapsulated ascorbic acid were studied for more than 60 days in PBS and it has been determined that PLGA completely degrades within this period, fully releasing all encapsulated ascorbic acid. The cytotoxicity of PLGA and PLGA/ascorbic acid 85/15% nanoparticles was examined with human hepatoma cell lines (HepG2 ECACC), in vitro. The obtained results indicate that neither PLGA nanospheres nor PLGA/ascorbic acid 85/15% nanoparticles significantly affected the viability of the HepG2 cells. The investigation of the distribution and pharmacokinetics of PLGA is crucial for the effective prediction of host responses to PLGA in particular applications. Thus we present a method of labeling PLGA nanospheres and PLGA/ascorbic acid 85/15 wt% nanoparticles by (99m)Tc which binds outside, leaving the cage intact. This enables a quick and convenient investigation of the pharmacological behavior and metabolism of PLGA. The biodistribution of (99m)Tc-labeled PLGA particles with and without encapsulated ascorbic acid after different periods of time of their installation into rats was examined. PLGA nanospheres with encapsulated ascorbic acid exhibit prolonged blood circulation accompanied by time-dependent reduction in the lungs, liver and spleen, and addition in the kidney, stomach and intestine. The samples were characterized by x-ray diffraction, scanning electron microscopy, stereological analysis, transmission electron microscopy, ultraviolet spectroscopy and instant thin layer chromatography.",
publisher = "Bristol : IOP Science",
journal = "Nanotechnology",
title = "An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres",
volume = "20",
number = "33",
doi = "10.1088/0957-4484/20/33/335102"
}
Stevanović, M., Maksin, T., Petković, J., Filipič, M.,& Uskoković, D. (2009). An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres.
NanotechnologyBristol : IOP Science., 20(33). 
https://doi.org/10.1088/0957-4484/20/33/335102
Stevanović M, Maksin T, Petković J, Filipič M, Uskoković D. An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres. Nanotechnology. 2009;20(33)
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