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Tomić, Sergej

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  • Tomić, Sergej (4)

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Toxicity of nanostructures

Čolić, Miodrag; Tomić, Sergej

(Belgrade : SASA, 2020)

TY  - CONF
AU  - Čolić, Miodrag
AU  - Tomić, Sergej
PY  - 2020
UR  - https://dais.sanu.ac.rs/123456789/9247
AB  - Due to their unique size (dimensions of 1 to 100 nm) and
physicochemical properties, nanomaterials have found numerous applications in
electronics, cosmetics, household appliances, energy storage, food industry, pharmacy and medicine. However, increased production of nanomaterials raises concern about their safety, not only for human beings but also for animals and the
environment. Numerous studies confirmed that nanoparticles (NPs) can exert
toxicity both, in vitro and in vivo, depending on their size, shape, surface area,
surface chemistry, concentration, dispersion, aggregation, route of administration
and many other factors, all of which are also relevant to desired biological properties of nanostructures. This review summarizes the main aspects of nanotoxicity in
vitro and in vivo, points out relevant tests in order to demonstrate the toxicity and
explains the significance of reactive oxygen species, as the main triggering factor
of NP cytotoxicity and genotoxicity, acting through the complex interplay between
NPs and cellular or genomic components, respectively. Special attention was devoted to the immunotoxic and immunomodulatory properties of NPs and their
relevance for production of less immunogenic nanostructures capable of avoiding
undesirable immune responses and the use of NPs as specific nanotherapeutics
for drug delivery and vaccination strategy. Finally, ecotoxicological aspects of NPs
are presented, showing why aquatic ecosystems are the most susceptible to environmental contamination and why studies on aquatic organisms are important for
translational nanotoxicology.
AB  - Због своје јединствене величине (димензије од 1 до 100 нм) и физичко-хемијских својстава, наноматеријали су нашли бројне примене у електроници, козметици, кућним апаратима, складиштењу енергије, прехрамбеној
индустрији, фармацији и медицини. Међутим, повећана производња наноматеријала изазива забринутост у погледу њихове сигурности, не само за
људска бића, већ и за животиње и животну средину. Бројна истраживања су
потврдила да наночестице (НЧ) могу да испољавају токсичност, in vitro и in
vivo, која зависи од њихове величине, облика, површине, хемијског састава
површине, концентрације, степена дисперзије и агрегације, начина примене
и многих других фактора. Сваки од њих је релевантан за очекивано биолошко својство одређене наноструктуре. Овај прегледни чланак приказује
сажето главне аспекте нанотоксичности in vitro и in vivo и истиче битне тестове којима се доказује токсичност НЧ. Посебно се наглашава значај реактивних врста кисеоника, као главног покретачког фактора цитотоксичности
и генотоксичности НЧ, које делују у сложеној међусобној интеракцији НЧ
са ћелијским, односно геномским компонентама. Посебан аспект се односи на имунотоксична и имуномодулацијска својства НЧ, што је од велике
важности за производњу мање имуногених наноструктура. На тај начин се може избећи нежељени имунски одговор и омогућити примена НЧ као
специфичних нанотерапеутика за доставу лекова и развој нових стратегија
вакцинације. На крају су приказани екотоксиколошки аспекти НЧ, указано
је зашто су водени екосистеми најосетљивији када се разматра контаминација животне средине наночестицама и зашто су истраживања на воденим
организмима важнa за област транслацијске нанотоксикологије.
PB  - Belgrade : SASA
C3  - Fascinating world of nanoscience and nanotechnology
T1  - Toxicity of nanostructures
T1  - Токсичност наноструктура
SP  - 71
EP  - 122
ER  - 
@conference{
author = "Čolić, Miodrag and Tomić, Sergej",
year = "2020",
url = "https://dais.sanu.ac.rs/123456789/9247",
abstract = "Due to their unique size (dimensions of 1 to 100 nm) and
physicochemical properties, nanomaterials have found numerous applications in
electronics, cosmetics, household appliances, energy storage, food industry, pharmacy and medicine. However, increased production of nanomaterials raises concern about their safety, not only for human beings but also for animals and the
environment. Numerous studies confirmed that nanoparticles (NPs) can exert
toxicity both, in vitro and in vivo, depending on their size, shape, surface area,
surface chemistry, concentration, dispersion, aggregation, route of administration
and many other factors, all of which are also relevant to desired biological properties of nanostructures. This review summarizes the main aspects of nanotoxicity in
vitro and in vivo, points out relevant tests in order to demonstrate the toxicity and
explains the significance of reactive oxygen species, as the main triggering factor
of NP cytotoxicity and genotoxicity, acting through the complex interplay between
NPs and cellular or genomic components, respectively. Special attention was devoted to the immunotoxic and immunomodulatory properties of NPs and their
relevance for production of less immunogenic nanostructures capable of avoiding
undesirable immune responses and the use of NPs as specific nanotherapeutics
for drug delivery and vaccination strategy. Finally, ecotoxicological aspects of NPs
are presented, showing why aquatic ecosystems are the most susceptible to environmental contamination and why studies on aquatic organisms are important for
translational nanotoxicology., Због своје јединствене величине (димензије од 1 до 100 нм) и физичко-хемијских својстава, наноматеријали су нашли бројне примене у електроници, козметици, кућним апаратима, складиштењу енергије, прехрамбеној
индустрији, фармацији и медицини. Међутим, повећана производња наноматеријала изазива забринутост у погледу њихове сигурности, не само за
људска бића, већ и за животиње и животну средину. Бројна истраживања су
потврдила да наночестице (НЧ) могу да испољавају токсичност, in vitro и in
vivo, која зависи од њихове величине, облика, површине, хемијског састава
површине, концентрације, степена дисперзије и агрегације, начина примене
и многих других фактора. Сваки од њих је релевантан за очекивано биолошко својство одређене наноструктуре. Овај прегледни чланак приказује
сажето главне аспекте нанотоксичности in vitro и in vivo и истиче битне тестове којима се доказује токсичност НЧ. Посебно се наглашава значај реактивних врста кисеоника, као главног покретачког фактора цитотоксичности
и генотоксичности НЧ, које делују у сложеној међусобној интеракцији НЧ
са ћелијским, односно геномским компонентама. Посебан аспект се односи на имунотоксична и имуномодулацијска својства НЧ, што је од велике
важности за производњу мање имуногених наноструктура. На тај начин се може избећи нежељени имунски одговор и омогућити примена НЧ као
специфичних нанотерапеутика за доставу лекова и развој нових стратегија
вакцинације. На крају су приказани екотоксиколошки аспекти НЧ, указано
је зашто су водени екосистеми најосетљивији када се разматра контаминација животне средине наночестицама и зашто су истраживања на воденим
организмима важнa за област транслацијске нанотоксикологије.",
publisher = "Belgrade : SASA",
journal = "Fascinating world of nanoscience and nanotechnology",
title = "Toxicity of nanostructures, Токсичност наноструктура",
pages = "71-122"
}

Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073

Filipović, Nenad; Veselinović, Ljiljana; Ražić, Slavica; Jeremić, Sanja; Filipič, Metka; Žegura, Bojana; Tomić, Sergej; Čolić, Miodrag; Stevanović, Magdalena

(2019)

@misc{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/5972",
journal = "Materials Science and Engineering C",
title = "Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073"
}

Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles

Filipović, Nenad; Veselinović, Ljiljana; Ražić, Slavica; Jeremić, Sanja; Filipič, Metka; Žegura, Bojana; Tomić, Sergej; Čolić, Miodrag; Stevanović, Magdalena

(Elsevier, 2019)

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/4600
AB  - Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles
SP  - 776
EP  - 789
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
ER  - 
@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/4600",
abstract = "Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "776-789",
volume = "96",
doi = "10.1016/j.msec.2018.11.073"
}
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Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles

Filipović, Nenad; Veselinović, Ljiljana; Ražić, Slavica; Jeremić, Sanja; Filipič, Metka; Žegura, Bojana; Tomić, Sergej; Čolić, Miodrag; Stevanović, Magdalena

(Elsevier, 2019)

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/4590
AB  - Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles
SP  - 776
EP  - 789
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
ER  - 
@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/4590",
abstract = "Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "776-789",
volume = "96",
doi = "10.1016/j.msec.2018.11.073"
}
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7