Sort By
Publication Year
Deposit Date
Title
Type
Access
Publication Year
2014 (2)
2013 (1)
2012 (2)

Stupar, Petar

Link to this page

Authority KeyName Variants
75437e48-8634-4a4b-9c31-0606d255497d
  • Stupar, Petar (5)
Projects

Author's Bibliography

Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species

Stupar, Petar; Pavlović, Vladimir B.; Nunić, Jana; Cundrič, Sandra; Filipič, Metka; Stevanović, Magdalena

(Paris : Association de pharmacie galenique industrielle, 2014)

TY  - JOUR
AU  - Stupar, Petar
AU  - Pavlović, Vladimir B.
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/425
AB  - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
PB  - Paris : Association de pharmacie galenique industrielle
T2  - Journal of Drug Delivery Science and Technology
T1  - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
SP  - 191
EP  - 197
VL  - 24
IS  - 2
DO  - 10.1016/S1773-2247(14)50031-7
ER  - 
@article{
author = "Stupar, Petar and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Stevanović, Magdalena",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/425",
abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.",
publisher = "Paris : Association de pharmacie galenique industrielle",
journal = "Journal of Drug Delivery Science and Technology",
title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species",
pages = "191-197",
volume = "24",
number = "2",
doi = "10.1016/S1773-2247(14)50031-7"
}
Stupar, P., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Stevanović, M. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species.
Journal of Drug Delivery Science and TechnologyParis : Association de pharmacie galenique industrielle., 24(2), 191-197. 
https://doi.org/10.1016/S1773-2247(14)50031-7
Stupar P, Pavlović VB, Nunić J, Cundrič S, Filipič M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. Journal of Drug Delivery Science and Technology. 2014;24(2):191-197
4
3
4

Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species

Stupar, Petar; Pavlović, Vladimir B.; Nunić, Jana; Cundrič, Sandra; Filipič, Metka; Stevanović, Magdalena

(Paris : Association de pharmacie galenique industrielle, 2014)

TY  - JOUR
AU  - Stupar, Petar
AU  - Pavlović, Vladimir B.
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/4671
AB  - A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.
PB  - Paris : Association de pharmacie galenique industrielle
T2  - Journal of Drug Delivery Science and Technology
T1  - Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species
SP  - 191
EP  - 197
VL  - 24
IS  - 2
DO  - 10.1016/S1773-2247(14)50031-7
ER  - 
@article{
author = "Stupar, Petar and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Stevanović, Magdalena",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/4671",
abstract = "A common limitation of using polymeric micro- and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro- and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. This paper underlines the combined outcomes of freeze drying method and physicochemical solvent/non-solvent approach to design biocompatible poly(epsilon-caprolactone) (PCL) nanospheres and evaluate influence of different cryoprotectants (glucose, saccharose, polyvinyl alcohol or polyglutamic acid) on the outcome of freeze-dried PCL particles. Samples were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and dynamic light scattering method (DLS). In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFHDA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels. It was found that cryoprotection with 1% glucose solution is an optimal for obtaining uniform, spherical but also biocompatible PCL nanoparticles for biomedical purposes.",
publisher = "Paris : Association de pharmacie galenique industrielle",
journal = "Journal of Drug Delivery Science and Technology",
title = "Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species",
pages = "191-197",
volume = "24",
number = "2",
doi = "10.1016/S1773-2247(14)50031-7"
}
Stupar, P., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Stevanović, M. (2014). Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species.
Journal of Drug Delivery Science and TechnologyParis : Association de pharmacie galenique industrielle., 24(2), 191-197. 
https://doi.org/10.1016/S1773-2247(14)50031-7
Stupar P, Pavlović VB, Nunić J, Cundrič S, Filipič M, Stevanović M. Development of lyophilized spherical particles of poly(epsilon-caprolactone) and examination of their morphology, cytocompatibility and influence on the formation of reactive oxygen species. Journal of Drug Delivery Science and Technology. 2014;24(2):191-197
4
3
4

Sinteza, karakterizacija i citokompatibilnost liofiliziranih sfernih čestica poli(ɛ-kaprolaktona) i ispitivanje njihovog uticaja na nastanak reaktivnih radikala kiseonika

Stupar, Petar

(Belgrade : University of Belgrade, Faculty of Physical Chemistry, 2013)

@mastersthesis{
author = "Stupar, Petar",
year = "2013",
url = "http://dais.sanu.ac.rs/123456789/757",
abstract = "Poli(ε-kaprolakton) (PCL) je biokompatibilan i biodegradabilan poliestar, koji se koristi za različite biomedicinske primene, kao što je kontrolisana dostava lekova, tkivno inženjerstvo, fiksacija koštanih fraktura i druge. Jedan od osnovnih uslova za primenu PCL polimera na polju kontrolisane dostave medikamenata, jesu odgovarajuće morfološke osobine čestica, tj. njihova sferičnost i velika uniformnost. 
Liofilizacija se koristi u farmaceutskoj industriji prilikom dobijanja najrazličitijih medikamenata, a predstavlja postupak sušenja pri redukovanom pritisku i pri vrlo niskim temperaturama. Iako omogućuje dobijanje čestica dovoljne stabilnosti za distribuciju i skladištenje, ovaj proces generiše izvesne negativne uticaje na strukturu samih formulacija. U cilju prevazilaženja ovakvih nezgoda, formulaciji se dodaju krioprotektanti, supstance koje štite čestice tokom zamrzavanja. Odabir krioprotektanta ni malo nije lak zadatak. Radi obezbeđivanja maksimalne protekcije čestica, potrebno je odabrati pravi tip krioprotektanta i optimizovati njegovu koncentraciju. 
U radu je opisana sinteza mikrosfera PCL-a fizičkohemijskom rastvarač/nerastvarač metodom i data je komparacija morfoloških karakteristika čestica sušenih u liofilizatoru, uz korišćenje različitih krioprotektanata: poli(L-glutaminske kiseline) (PGA), poli(vinil alkohola) (PVA), saharoze ili glukoze. Uzorci su karakterisani metodama infracrvene spektroskopije sa Fourierovom transformacijom (FT-IR), skenirajuće elektronske mikroskopije (SEM) kao i metodom analize difrakcije laserske svetlosti na česticama (DLS). U analizi svojstava materijala koji je procesiran u cilju potencijalne primene u oblasti biomedicine, veoma je važno ispitati njegovu citokompatibilnost, odnosno meru u kojoj je materijal podesan da koegzistira u prisustvu ćelija, tako da ne utiče na njihov prirodni životni proces, u smislu podsticanja procesa degeneracije i umiranja. Takođe, veoma je važno ispitati i uticaj materijala na prekomerno obrazovanje reaktivnih kiseoničnih vrsta (reactive oxygen species - ROS) i nastanak oksidativnog stresa, koji se dovodi u vezu sa mnogim oboljenjima. Citokompatibilnost uzoraka je ispitana MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolijum bromid) testom, dok je ROS efekat ispitan DCFH-DA (2′-7′-dihlorodihidrofluorescein diacetat) testom., Poly(ε-caprolactone) (PCL) is a biocompatible and biodegradable polyester, extensively investigated for applications in controlled drug delivery, tissue engineering, bone fracture fixation etc. One of the crucial conditions for the application of polymers in controlled drug delivery are morphological characteristics of the particles, their sphericity and a high uniformity. 
Freeze drying, also termed lyophilization, is a process of drying in conditions of reduced pressure and very low temperatures. Although this technique was considered as a good method to conserve the integrity of particulate systems, various stresses are generated during the process. To protect nanoparticles from stresses and subsequent aggregation, a cryoprotectant or lyoprotectant is generally used. The selection of a proper cryoprotectant is not straightforward. In some cases, increasing cryoprotectant concentration to a certain level may eventually reach a limit of stabilization or even destabilize nanoparticles. In general, the type of cryoprotectant must be selected and its concentration must be optimized to ensure a maximum stabilization of nanoparticles. 
The aim of this study was to produce PCL spherical particles using physico-chemical solvent/nonsolvent method, and to evaluate different cryoprotective excipients that were used in order to increase stability of the particles and protect them from freezing stress. Sugars, such as glucose and sucrose, and polymers (PVA and PGA), were added to the formulation to test the effects on the outcome of freeze-dried poly(ε-caprolactone) micro- and nanospheres. Samples were characterized using Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and Dynamic Light Scattering method (DLS). In the analysis of properties of materials processed for applications in biomedicine, it is of crucial importance to test its cytocompatibility, the extent at which the material is able to coexist in the presence of cells without interrupting their living process in terms of promoting degeneration and death. Furthermore, it is very important to investigate the effects on excessive production of reactive oxygen species (ROS) and the oxidative stress, which is responsible for many diseases. In vitro studies used, include MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), testing cytotoxicity as the quality of being toxic to cells, and DCFH-DA assay (2’,7’-dichlordihydrofluorescein-diacetate), testing the possible increase in ROS levels.",
publisher = "Belgrade : University of Belgrade, Faculty of Physical Chemistry",
title = "Sinteza, karakterizacija i citokompatibilnost liofiliziranih sfernih čestica poli(ɛ-kaprolaktona) i ispitivanje njihovog uticaja na nastanak reaktivnih radikala kiseonika"
}
,& Stupar, P. (2013). Sinteza, karakterizacija i citokompatibilnost liofiliziranih sfernih čestica poli(ɛ-kaprolaktona) i ispitivanje njihovog uticaja na nastanak reaktivnih radikala kiseonika.
nullBelgrade : University of Belgrade, Faculty of Physical Chemistry., null. 
Stupar P. Sinteza, karakterizacija i citokompatibilnost liofiliziranih sfernih čestica poli(ɛ-kaprolaktona) i ispitivanje njihovog uticaja na nastanak reaktivnih radikala kiseonika. 2013;

Freeze-drying method to produce a range of PCL particles with tailored morphological properties

Filipović, Nenad; Stevanović, Magdalena; Stupar, Petar; Petković, Jana; Filipič, Metka; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia, 2012)

TY  - CONF
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Stupar, Petar
AU  - Petković, Jana
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/456
AB  - Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1  - Freeze-drying method to produce a range of PCL particles with tailored morphological properties
SP  - 124
EP  - 124
ER  - 
@conference{
author = "Filipović, Nenad and Stevanović, Magdalena and Stupar, Petar and Petković, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/456",
abstract = "Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Freeze-drying method to produce a range of PCL particles with tailored morphological properties",
pages = "124-124"
}
Filipović, N., Stevanović, M., Stupar, P., Petković, J., Filipič, M.,& Uskoković, D. (2012). Freeze-drying method to produce a range of PCL particles with tailored morphological properties.
The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of AbstractsBelgrade : Materials Research Society of Serbia., null, 124-124. 
Filipović N, Stevanović M, Stupar P, Petković J, Filipič M, Uskoković D. Freeze-drying method to produce a range of PCL particles with tailored morphological properties. The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:124-124

Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres

Stupar, Petar; Stevanović, Magdalena; Filipović, Nenad; Pavlović, Vladimir B.; Nunić, Jana; Cundrič, Sandra; Filipič, Metka; Uskoković, Dragan

(Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade, 2012)

TY  - CONF
AU  - Stupar, Petar
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Pavlović, Vladimir B.
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/531
AB  - A common limitation of using polymeric micro and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. The aim of this study was to evaluate different cryoprotectants (protective excipients that are usually added to increase stability upon storage and protect the particles from freezing stress): sugars (glucose and sucrose) and polymers (PVA and PGA), on the outcome of freeze-dried poly(ε-caprolactone) micro and nanospheres. The best freeze-drying results in terms of morphological characteristics, analyzed with SEM, were achieved with glucose at concentration of 1%. The FTIR analysis confirmed that the molecular structure of PCL particles remained the same after the addition cryoprotectants.
PB  - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1  - Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres
SP  - 104
EP  - 104
ER  - 
@conference{
author = "Stupar, Petar and Stevanović, Magdalena and Filipović, Nenad and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/531",
abstract = "A common limitation of using polymeric micro and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. The aim of this study was to evaluate different cryoprotectants (protective excipients that are usually added to increase stability upon storage and protect the particles from freezing stress): sugars (glucose and sucrose) and polymers (PVA and PGA), on the outcome of freeze-dried poly(ε-caprolactone) micro and nanospheres. The best freeze-drying results in terms of morphological characteristics, analyzed with SEM, were achieved with glucose at concentration of 1%. The FTIR analysis confirmed that the molecular structure of PCL particles remained the same after the addition cryoprotectants.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres",
pages = "104-104"
}
Stupar, P., Stevanović, M., Filipović, N., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D. (2012). Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres.
Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of AbstractsBelgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., null, 104-104. 
Stupar P, Stevanović M, Filipović N, Pavlović VB, Nunić J, Cundrič S, Filipič M, Uskoković D. Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres. Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:104-104