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Filipović, Miloš

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  • Filipović, Miloš (5)
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Author's Bibliography

Selenium nanoparticles as a potential candidate in cancer treatment

Filipović, Nenad; Ninić, Jana; Filipič, Metka; Filipović, Miloš; Stevanović, Magdalena

(Rovinj : International Association of Physical Chemists, 2015)

TY  - CONF
AU  - Filipović, Nenad
AU  - Ninić, Jana
AU  - Filipič, Metka
AU  - Filipović, Miloš
AU  - Stevanović, Magdalena
PY  - 2015
UR  - http://dais.sanu.ac.rs/123456789/858
AB  - The broad spectrum of selenium applications in pharmacy and medicine has been known for a while and strongly depends on its chemical form, size and shape. However, the use of Se often requires consumption over the long period, so the toxicity of Se is always a crucial concern. Majority of available pharmaceutical products contain organic forms of selenium or its salts, but recently, when it comes to cancer treatment, elemental selenium nanoparticles (SeNPs) have emerged as a novel selenium source with the advantage of reduced risk of selenium toxicity, but with same bioavailability and efficacy in increasing the activities of selenoenzimes. 
In this work we are presenting the fast, reproducible method for producing stable colloidal suspension of amorphous SeNPs (<80 nm). These SeNPS were successful incorporated within PCL microspheres by combining the high speed homogenization and the precipitation in a solvent/non-solvent system. The obtained PCL/SeNPs were characterized by Fourier transform infrared spectroscopy (FTIR), electron microscopy (SEM and TEM), X-ray diffraction (XRD) and thermal analysis methods (TGA-DTA). The cytotoxicity and the formation of intracellular reactive oxygen species of SeNPs as well as of PCL/SeNPs were investigated employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and using a fluorescent probe (DCFDA test) respectively. Both systems have shown good biocompatibility. The anticancer activity of SeNPs was examined on the HeLa cell line and it was demonstrated that SeNPs exhibits strong, a dose dependent, anticancer activity by preventing further HeLa cells growth and division. Bearing in mind that PCL is well known biodegradable polymer with low degradation rate, it is our opinion that PCL/SeNPs possess a great potential for cancer treatment.
PB  - Rovinj : International Association of Physical Chemists
C3  - Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK
T1  - Selenium nanoparticles as a potential candidate in cancer treatment
ER  - 
@conference{
author = "Filipović, Nenad and Ninić, Jana and Filipič, Metka and Filipović, Miloš and Stevanović, Magdalena",
year = "2015",
url = "http://dais.sanu.ac.rs/123456789/858",
abstract = "The broad spectrum of selenium applications in pharmacy and medicine has been known for a while and strongly depends on its chemical form, size and shape. However, the use of Se often requires consumption over the long period, so the toxicity of Se is always a crucial concern. Majority of available pharmaceutical products contain organic forms of selenium or its salts, but recently, when it comes to cancer treatment, elemental selenium nanoparticles (SeNPs) have emerged as a novel selenium source with the advantage of reduced risk of selenium toxicity, but with same bioavailability and efficacy in increasing the activities of selenoenzimes. 
In this work we are presenting the fast, reproducible method for producing stable colloidal suspension of amorphous SeNPs (<80 nm). These SeNPS were successful incorporated within PCL microspheres by combining the high speed homogenization and the precipitation in a solvent/non-solvent system. The obtained PCL/SeNPs were characterized by Fourier transform infrared spectroscopy (FTIR), electron microscopy (SEM and TEM), X-ray diffraction (XRD) and thermal analysis methods (TGA-DTA). The cytotoxicity and the formation of intracellular reactive oxygen species of SeNPs as well as of PCL/SeNPs were investigated employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and using a fluorescent probe (DCFDA test) respectively. Both systems have shown good biocompatibility. The anticancer activity of SeNPs was examined on the HeLa cell line and it was demonstrated that SeNPs exhibits strong, a dose dependent, anticancer activity by preventing further HeLa cells growth and division. Bearing in mind that PCL is well known biodegradable polymer with low degradation rate, it is our opinion that PCL/SeNPs possess a great potential for cancer treatment.",
publisher = "Rovinj : International Association of Physical Chemists",
journal = "Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK",
title = "Selenium nanoparticles as a potential candidate in cancer treatment"
}
Filipović, N., Ninić, J., Filipič, M., Filipović, M.,& Stevanović, M. (2015). Selenium nanoparticles as a potential candidate in cancer treatment.
Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPKRovinj : International Association of Physical Chemists., null. 
Filipović N, Ninić J, Filipič M, Filipović M, Stevanović M. Selenium nanoparticles as a potential candidate in cancer treatment. Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK. 2015;

Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality

Stevanović, Magdalena; Nunić, Jana; Choi, Jonghoon; Filipović, Miloš; Uskoković, Dragan; Tsotakos, Theodore; Fragogeorgi, Eirini; Psimadas, Dimitris; Palamaris, Lazaros; Loudos, George

(Belgrade : Materials Research Society of Serbia, 2014)

TY  - CONF
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Choi, Jonghoon
AU  - Filipović, Miloš
AU  - Uskoković, Dragan
AU  - Tsotakos, Theodore
AU  - Fragogeorgi, Eirini
AU  - Psimadas, Dimitris
AU  - Palamaris, Lazaros
AU  - Loudos, George
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/584
AB  - Selenium (Se) is an essential trace element with important physiological functions and extensive pharmacological actions. The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials. Uniform, stable, amorphous selenium nanoparticles (SeNps) have been synthesized and additionally encapsulated within spherical PLGA particles (PLGA/SeNps). The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell. Synthesized particles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts
T1  - Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality
SP  - 38
EP  - 38
ER  - 
@conference{
author = "Stevanović, Magdalena and Nunić, Jana and Choi, Jonghoon and Filipović, Miloš and Uskoković, Dragan and Tsotakos, Theodore and Fragogeorgi, Eirini and Psimadas, Dimitris and Palamaris, Lazaros and Loudos, George",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/584",
abstract = "Selenium (Se) is an essential trace element with important physiological functions and extensive pharmacological actions. The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials. Uniform, stable, amorphous selenium nanoparticles (SeNps) have been synthesized and additionally encapsulated within spherical PLGA particles (PLGA/SeNps). The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell. Synthesized particles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts",
title = "Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality",
pages = "38-38"
}
Stevanović, M., Nunić, J., Choi, J., Filipović, M., Uskoković, D., Tsotakos, T., Fragogeorgi, E., Psimadas, D., Palamaris, L.,& Loudos, G. (2014). Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality.
The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of AbstractsBelgrade : Materials Research Society of Serbia., null, 38-38. 
Stevanović M, Nunić J, Choi J, Filipović M, Uskoković D, Tsotakos T, Fragogeorgi E, Psimadas D, Palamaris L, Loudos G. Poly (DL-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles as a system with therapeutic functionality. The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts. 2014;:38-38

Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities

Stevanović, Magdalena; Uskoković, Vuk; Filipović, Miloš; Škapin, Srečo Davor; Uskoković, Dragan

(2013)

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Uskoković, Vuk
AU  - Filipović, Miloš
AU  - Škapin, Srečo Davor
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/15986
AB  - The global rise in the resistance of pathogens to conventional antibiotics has created an intensive search for alternative materials with antimicrobial properties. This study is performed with an intention to investigate the combined effects of poly(L-glutamic acid)-capped silver nanoparticles (AgNpPGA) and ascorbic acid (AscH) encapsulated within freeze-dried poly(lactide-co-glycolide) (PLGA) nanospheres to obtain a nanomaterial with simultaneous osteoinductive, antioxidative, and prolonged antimicrobial properties. The influence of PLGA/AgNpPGA/AscH particles on (i) viability and super-oxide production of human umbilical vein endothelial cells in vitro, (ii) morphology and expression of osteogenic markers in osteoblastic MC3T3-E1 cells in vitro, and (iii) antimicrobial activity against a Gram-positive bacterium, methicillin-resistant Staphylococcus aureus, and a Gram-negative bacterium, Escherichia coli, was investigated. PLGA/AgNpPGA/AscH nanoparticles showed a superior and extended antibacterial activity against both types of bacteria. The nanoparticles appeared to be capable of delivering ascorbate to the cells, which was evidenced by the significant decrease in the level of superoxides in human umbilical vein endothelial cells and which could have a therapeutic potential in preventing oxidative stress. PLGA/AgNpPGA/AscH nanoparticles had a positive effect on MC3T3-E1 osteoblastic cells in vitro, promoting: (i) an intimate contact with the cells and preservation of their healthy morphologies; (ii) unreduced cell viability; and (iii) multiple-fold upregulation of two osteogenic markers: osteocalcin and type I procollagen. It is concluded that PLGA/AgNpPGA/AscH nanospheres present a promising new material for the treatment of infections and use in wound dressings and other prophylactic applications.
T2  - ACS Applied Materials and Interfaces
T1  - Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities
SP  - 9034
EP  - 9042
VL  - 5
IS  - 18
DO  - 10.1021/am402237g
ER  - 
@article{
author = "Stevanović, Magdalena and Uskoković, Vuk and Filipović, Miloš and Škapin, Srečo Davor and Uskoković, Dragan",
year = "2013",
url = "http://dais.sanu.ac.rs/123456789/15986",
abstract = "The global rise in the resistance of pathogens to conventional antibiotics has created an intensive search for alternative materials with antimicrobial properties. This study is performed with an intention to investigate the combined effects of poly(L-glutamic acid)-capped silver nanoparticles (AgNpPGA) and ascorbic acid (AscH) encapsulated within freeze-dried poly(lactide-co-glycolide) (PLGA) nanospheres to obtain a nanomaterial with simultaneous osteoinductive, antioxidative, and prolonged antimicrobial properties. The influence of PLGA/AgNpPGA/AscH particles on (i) viability and super-oxide production of human umbilical vein endothelial cells in vitro, (ii) morphology and expression of osteogenic markers in osteoblastic MC3T3-E1 cells in vitro, and (iii) antimicrobial activity against a Gram-positive bacterium, methicillin-resistant Staphylococcus aureus, and a Gram-negative bacterium, Escherichia coli, was investigated. PLGA/AgNpPGA/AscH nanoparticles showed a superior and extended antibacterial activity against both types of bacteria. The nanoparticles appeared to be capable of delivering ascorbate to the cells, which was evidenced by the significant decrease in the level of superoxides in human umbilical vein endothelial cells and which could have a therapeutic potential in preventing oxidative stress. PLGA/AgNpPGA/AscH nanoparticles had a positive effect on MC3T3-E1 osteoblastic cells in vitro, promoting: (i) an intimate contact with the cells and preservation of their healthy morphologies; (ii) unreduced cell viability; and (iii) multiple-fold upregulation of two osteogenic markers: osteocalcin and type I procollagen. It is concluded that PLGA/AgNpPGA/AscH nanospheres present a promising new material for the treatment of infections and use in wound dressings and other prophylactic applications.",
journal = "ACS Applied Materials and Interfaces",
title = "Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities",
pages = "9034-9042",
volume = "5",
number = "18",
doi = "10.1021/am402237g"
}
Stevanović, M., Uskoković, V., Filipović, M., Škapin, S. D.,& Uskoković, D. (2013). Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities.
ACS Applied Materials and Interfaces, 5(18), 9034-9042. 
https://doi.org/10.1021/am402237g
Stevanović M, Uskoković V, Filipović M, Škapin SD, Uskoković D. Composite PLGA/AgNpPGA/AscH Nanospheres with Combined Osteoinductive, Antioxidative, and Antimicrobial Activities. ACS Applied Materials and Interfaces. 2013;5(18):9034-9042
31
32
30

Composite PLGA/AgNpPGA/AscH nanospheres with combined osteoinductive, antioxidative and antimicrobial activities

Stevanović, Magdalena; Uskoković, Vuk; Filipović, Miloš; Škapin, Srečo Davor; Uskoković, Dragan

(American Chemical Society, 2013)

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Uskoković, Vuk
AU  - Filipović, Miloš
AU  - Škapin, Srečo Davor
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/381
AB  - The global rise in the resistance of pathogens to conventional antibiotics has created an intensive search for alternative materials with antimicrobial properties. This study is performed with an intention to investigate the combined effects of poly(l-glutamic acid)-capped silver nanoparticles (AgNpPGA) and ascorbic acid (AscH) encapsulated within freeze-dried poly(lactide-co-glycolide) (PLGA) nanospheres to obtain a nanomaterial with simultaneous osteoinductive, antioxidative, and prolonged antimicrobial properties. The influence of PLGA/AgNpPGA/AscH particles on (i) viability and superoxide production of human umbilical vein endothelial cells in vitro, (ii) morphology and expression of osteogenic markers in osteoblastic MC3T3-E1 cells in vitro, and (iii) antimicrobial activity against a Gram-positive bacterium, methicillin-resistant Staphylococcus aureus, and a Gram-negative bacterium, Escherichia coli, was investigated. PLGA/AgNpPGA/AscH nanoparticles showed a superior and extended antibacterial activity against both types of bacteria. The nanoparticles appeared to be capable of delivering ascorbate to the cells, which was evidenced by the significant decrease in the level of superoxides in human umbilical vein endothelial cells and which could have a therapeutic potential in preventing oxidative stress. PLGA/AgNpPGA/AscH nanoparticles had a positive effect on MC3T3-E1 osteoblastic cells in vitro, promoting: (i) an intimate contact with the cells and preservation of their healthy morphologies; (ii) unreduced cell viability; and (iii) multiple-fold upregulation of two osteogenic markers: osteocalcin and type I procollagen. It is concluded that PLGA/AgNpPGA/AscH nanospheres present a promising new material for the treatment of infections and use in wound dressings and other prophylactic applications.
PB  - American Chemical Society
T2  - ACS Applied Materials and Interfaces
T1  - Composite PLGA/AgNpPGA/AscH nanospheres with combined osteoinductive, antioxidative and antimicrobial activities
SP  - 9034
EP  - 9042
VL  - 5
IS  - 18
DO  - 10.1021/am402237g
ER  - 
@article{
author = "Stevanović, Magdalena and Uskoković, Vuk and Filipović, Miloš and Škapin, Srečo Davor and Uskoković, Dragan",
year = "2013",
url = "http://dais.sanu.ac.rs/123456789/381",
abstract = "The global rise in the resistance of pathogens to conventional antibiotics has created an intensive search for alternative materials with antimicrobial properties. This study is performed with an intention to investigate the combined effects of poly(l-glutamic acid)-capped silver nanoparticles (AgNpPGA) and ascorbic acid (AscH) encapsulated within freeze-dried poly(lactide-co-glycolide) (PLGA) nanospheres to obtain a nanomaterial with simultaneous osteoinductive, antioxidative, and prolonged antimicrobial properties. The influence of PLGA/AgNpPGA/AscH particles on (i) viability and superoxide production of human umbilical vein endothelial cells in vitro, (ii) morphology and expression of osteogenic markers in osteoblastic MC3T3-E1 cells in vitro, and (iii) antimicrobial activity against a Gram-positive bacterium, methicillin-resistant Staphylococcus aureus, and a Gram-negative bacterium, Escherichia coli, was investigated. PLGA/AgNpPGA/AscH nanoparticles showed a superior and extended antibacterial activity against both types of bacteria. The nanoparticles appeared to be capable of delivering ascorbate to the cells, which was evidenced by the significant decrease in the level of superoxides in human umbilical vein endothelial cells and which could have a therapeutic potential in preventing oxidative stress. PLGA/AgNpPGA/AscH nanoparticles had a positive effect on MC3T3-E1 osteoblastic cells in vitro, promoting: (i) an intimate contact with the cells and preservation of their healthy morphologies; (ii) unreduced cell viability; and (iii) multiple-fold upregulation of two osteogenic markers: osteocalcin and type I procollagen. It is concluded that PLGA/AgNpPGA/AscH nanospheres present a promising new material for the treatment of infections and use in wound dressings and other prophylactic applications.",
publisher = "American Chemical Society",
journal = "ACS Applied Materials and Interfaces",
title = "Composite PLGA/AgNpPGA/AscH nanospheres with combined osteoinductive, antioxidative and antimicrobial activities",
pages = "9034-9042",
volume = "5",
number = "18",
doi = "10.1021/am402237g"
}
Stevanović, M., Uskoković, V., Filipović, M., Škapin, S. D.,& Uskoković, D. (2013). Composite PLGA/AgNpPGA/AscH nanospheres with combined osteoinductive, antioxidative and antimicrobial activities.
ACS Applied Materials and InterfacesAmerican Chemical Society., 5(18), 9034-9042. 
https://doi.org/10.1021/am402237g
Stevanović M, Uskoković V, Filipović M, Škapin SD, Uskoković D. Composite PLGA/AgNpPGA/AscH nanospheres with combined osteoinductive, antioxidative and antimicrobial activities. ACS Applied Materials and Interfaces. 2013;5(18):9034-9042
31
32
30

In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases

Stevanović, Magdalena; Nunić, Jana; Choi, Jonghoon; Filipović, Miloš; Uskoković, Dragan; Tsotakos, Theodore; Fragogeorgi, Eirini; Psimadas, Dimitris; Palamaris, Lazaros; Loudos, George

(Athens, 2013)

TY  - CONF
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Choi, Jonghoon
AU  - Filipović, Miloš
AU  - Uskoković, Dragan
AU  - Tsotakos, Theodore
AU  - Fragogeorgi, Eirini
AU  - Psimadas, Dimitris
AU  - Palamaris, Lazaros
AU  - Loudos, George
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/855
AB  - The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials [1, 2] suggesting that anti-tumor effect mechanisms of selenium include induction of apoptosis, inhibition of cell proliferation, protection against oxidative stress, and stimulation of immune system.
Herein we demonstrate a simple and quick synthesis of uniform, stable, amorphous selenium nanoparticles (SeNps), using ascorbic acid as the reduction agent. The choice of an appropriate stabilizer and reducing agent for preparation of stable selenium nanoparticles is very important. We used bovine serum albumin (BSA) as an organic layer for selenium nanoparticles, i.e., as a capping agent to make them more biocompatibile and protect them from agglomeration in the suspension medium.
SeNps were additionally encapsulated within spherical PLGA particles (PLGA/SeNps). One of the most important requirements for the controlled and balanced release of the drug in the body is ideal spherical shape of the particles and narrow distribution of their sizes. The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell.
The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. Synthesized nanoparticles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method [3]. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.

Acknowledgements
This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia, under Grant No. III45004: Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them. Presented were the results of a study also supported by the COST Action TD1004.

References
1. Popova, N. V. Cancer Lett. 2002, 179, 39–42.
2. Li, S.; Zhou, Y.; Wang, R.; Zhang, H.; Dong, Y.; Ip, C. Mol. Cancer Ther. 2007, 6, 1031–1038.
3. Psimadas, D.; Baldi, G.; Ravagli, C.; Bouziotis, P.; Xanthopoulos, S.; Francini, M.; Georgoulias, P.; Loudos, G.; J. Biom. Nan., 2012, 8, 4, 575-585.
PB  - Athens
C3  - Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013
T1  - In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases
ER  - 
@conference{
author = "Stevanović, Magdalena and Nunić, Jana and Choi, Jonghoon and Filipović, Miloš and Uskoković, Dragan and Tsotakos, Theodore and Fragogeorgi, Eirini and Psimadas, Dimitris and Palamaris, Lazaros and Loudos, George",
year = "2013",
url = "http://dais.sanu.ac.rs/123456789/855",
abstract = "The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials [1, 2] suggesting that anti-tumor effect mechanisms of selenium include induction of apoptosis, inhibition of cell proliferation, protection against oxidative stress, and stimulation of immune system.
Herein we demonstrate a simple and quick synthesis of uniform, stable, amorphous selenium nanoparticles (SeNps), using ascorbic acid as the reduction agent. The choice of an appropriate stabilizer and reducing agent for preparation of stable selenium nanoparticles is very important. We used bovine serum albumin (BSA) as an organic layer for selenium nanoparticles, i.e., as a capping agent to make them more biocompatibile and protect them from agglomeration in the suspension medium.
SeNps were additionally encapsulated within spherical PLGA particles (PLGA/SeNps). One of the most important requirements for the controlled and balanced release of the drug in the body is ideal spherical shape of the particles and narrow distribution of their sizes. The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell.
The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. Synthesized nanoparticles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method [3]. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.

Acknowledgements
This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia, under Grant No. III45004: Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them. Presented were the results of a study also supported by the COST Action TD1004.

References
1. Popova, N. V. Cancer Lett. 2002, 179, 39–42.
2. Li, S.; Zhou, Y.; Wang, R.; Zhang, H.; Dong, Y.; Ip, C. Mol. Cancer Ther. 2007, 6, 1031–1038.
3. Psimadas, D.; Baldi, G.; Ravagli, C.; Bouziotis, P.; Xanthopoulos, S.; Francini, M.; Georgoulias, P.; Loudos, G.; J. Biom. Nan., 2012, 8, 4, 575-585.",
publisher = "Athens",
journal = "Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013",
title = "In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases"
}
Stevanović, M., Nunić, J., Choi, J., Filipović, M., Uskoković, D., Tsotakos, T., Fragogeorgi, E., Psimadas, D., Palamaris, L.,& Loudos, G. (2013). In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases.
Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013Athens., null. 
Stevanović M, Nunić J, Choi J, Filipović M, Uskoković D, Tsotakos T, Fragogeorgi E, Psimadas D, Palamaris L, Loudos G. In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases. Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013. 2013;