@conference{
author = "Ganić, Tea and Cvetković, Stefana and Vuletić, Stefana and Stević, Tatjana and Stevanović, Magdalena and Tomić, Nina and Nikolić, Biljana and Mitić Ćulafić, Dragana",
year = "2023",
abstract = "Cimet je jedna od najpoznatijih aromatičnih biljaka, koje je najčešće korišćena kao začin. Prepoznat je kao potencijalni terapeutik u narodnoj medicini, zbog čega se dugo koristi za lečenje različitih infekcija. Etarska ulja su sekundarni metaboliti koje sintetišu aromatične biljke. Za etarsko ulje cimeta (CEO) su definisane različite biološke aktivnosti, međutim fizičkohemijske karakteristike ulja su slaba rastvorljivost u vodi i laka isparljivost, što ograničava njihovu primenu. Iz tog razloga, formulacija emulzija etarskih ulja predstavlja dobro rešenje za prevazilaženje problema njihove primene. Iako se CEO smatra bezbednim za upotrebu, rezultati istraživanja variraju, ukazujući na neophodnost daljih toksikoloških istraživanja. Cilj ovog rada je bila komparativna analiza biološke aktivnosti i in vitro toksičnosti CEO i njegove emulzije (EM). Antioksidativni potencijal je ispitivan DPPH testom. MTT test je primenjen u cilju procene citotoksičnog efekta CEO i EM na fibroblastima pluća fetusa (MRC–5). Na istoj ćelijskoj liniji, ispitivan je nivo oštećenja molekula DNK, primenom alkalnog komet testa. Na osnovu rezltata DPPH testa, IC50 vrednost za CEO je bila 320 μg/mL, dok je za EM bila 54 μg/mL. Na ćelijskoj liniji MRC–5, IC50 vrednosti dobijene MTT testom su iznosile 0,13 mg/mL za CEO i 0,025 mg/mL za EM. CEO i EM nisu pokazali genotoksični potencijal. Uzimajući sve prethodno navedeno u obzir, CEO i EM, kao jaki antioksidanti koji pokazuju odsustvo genotoksičnosti i slabu citotoksičnost., Cinnamon is among most popular aromatic plants, generally used as a spice. Over the centuries it was recognized as a potential therapeutic for different kind of infections. Essential oils are aromatic plants’ secondary metabolites. Cinnamon essential oil (CEO) possess many favorable biological activities, but its physicochemical properties, as an oil, are poor solubility in water and high volatility, which limits its application. For that reason, preparation of essential oils’ emulsions seems to be solution to overcome applicability problems. Even though CEO is one of the oils recognized as safe to be used, the results from different studies vary, indicating necessity to further study its toxicological properties. The aim of this study was to comparatively analyze CEO and its emulsion (EM) biological activity and in vitro toxicity. Antioxidative potential was investigated using DPPH assay. The MTT assay was applied for the estimation of cytotoxic effects of CEO and EM on fetal fibroblasts (MRC-5) cell line. On the same cell line, level of DNA damage was evaluated, using alkaline comet assay. According to the DPPH assay results, IC50 value for CEO was 320 μg/mL, for EM was 54 μg/mL. On MRC-5 cell line, IC50 values were 0.13 mg/mL for CEO and 0.025 mg/mL for EM. Both CEO and EM didn’t exhibit potential to induce DNA damage. Taking all above mention into account, as strong antioxidants without genotoxicity and with weak cytotoxicity, CEO and EM could be considered as good candidates for further investigation of biological activities and potential for human use.",
publisher = "Beograd : Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : Present and future of toxicology: challenges and opportunities : 10-12 May 2023 Belgrade : abstract book",
title = "Procena antioksidativnog, citotoksičnog i genotoksičnog efekta etarskog ulja cimeta i njegove emulzije, Evaluation of antioxidative, cytotoxic and genotoxic effect of cinnamon essential oil and its emulsion",
pages = "152-153",
url = "https://hdl.handle.net/21.15107/rcub_dais_16246"
}