TY  - CONF
AU  - Dinić, Ivana
AU  - Vuković, Marina
AU  - Bukumira, Marta
AU  - Rabasović, Mihailo
AU  - Mančić, Lidija
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16779
AB  - Imaging of biological processes are of the great importance for early diagnostic of diseases and for understanding the disease pathway in the human body [1]. In order to improve image contrast and resolution, different optical fluorescent probes, such as florescent dyes, fluorescent proteins and quantum dots, have been developed. However, their application is somewhat restricted by photobleaching, cell autofluorescence, and small tissue penetration depth [2]. Lanthanide doped up-conversion nanoparticles (UCNPs) owing to their unique optical properties and high photochemical stability, represents a promising material for superior optical diagnostic and drug delivery systems [3]. Due to large anti-Stocks shift, UCNPs have converse excitation and emission profiles: under excitation by near-infrared light (NIR), they are able to emit visible or UV photons whit a shorter wavelength [4,5]. Hexagonal-phase NaYF4:Yb,Er nanocrystals are the best NIR-to-visible up-converting materials to date, but YF3 phase due to its wider optical transmission window, and the minimization of the excited state quenching of doped ions, is considered to be promising material for application in biolabeling as well. In this work, biocompatible YF3:YbEr and GdF3:YbEr mesocrystals were prepared by one-pot solvothermal synthesis in the presence of chitosan, and are used successfully as biolabeling agents. The crystallinity and phase purity of the obtained powders were confirmed by X-ray powder diffraction which revealed that both samples are with the orthorhombic Pnma crystal structure, and monophase. Homogeneous distribution of dopant ions in the host matrix was confirmed by STEM/EDS mapping (not shown). SEM and TEM analysis implied that particles possess morphology similar to the unshelled peanuts, which are built from smaller monocrystals (Figs 1a and b). It was shown that their capacity toward self-organization is determined by the amount of surface-preserved chitosan ligands, which presence is confirmed by FTIR spectroscopy. Chitosan presence provides biocompatibility, thus the viability of both, MRC- 5 lung fibroblast, and A549 lung carcinoma cells was highly preserved (>80%), after 24 h of incubation with the synthetized samples (50μg mL-1). To monitor the intracellular uptake, cells incubated with particles were further analyzed by laser scanning microscopy under 980nm excitation. A strong up-conversion emission (green spots at Figs 1c and d), indicated that particles (green spots) are positioned in the cells cytoplasmic area adjacent to the plasma membrane, in both cell lines. Such positioning, without disturbing cells nuclei confirms their capacity to be used as NIR cell labeling agents.
PB  - Belgrade : Serbian Academy of Sciences and Arts
C3  - Program & Book of Abstracts / Third International Conference ELMINA 2024, Belgrade, Serbia, September 9th -13th, 2024
T1  - Biocompatible Up-converting Yb/Er Doped Ln-fluoride Mesocrystals for Cell Labeling
SP  - 176
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_dais_16779
ER  - 

@conference{
author = "Dinić, Ivana and Vuković, Marina and Bukumira, Marta and Rabasović, Mihailo and Mančić, Lidija",
year = "2024",
abstract = "Imaging of biological processes are of the great importance for early diagnostic of diseases and for understanding the disease pathway in the human body [1]. In order to improve image contrast and resolution, different optical fluorescent probes, such as florescent dyes, fluorescent proteins and quantum dots, have been developed. However, their application is somewhat restricted by photobleaching, cell autofluorescence, and small tissue penetration depth [2]. Lanthanide doped up-conversion nanoparticles (UCNPs) owing to their unique optical properties and high photochemical stability, represents a promising material for superior optical diagnostic and drug delivery systems [3]. Due to large anti-Stocks shift, UCNPs have converse excitation and emission profiles: under excitation by near-infrared light (NIR), they are able to emit visible or UV photons whit a shorter wavelength [4,5]. Hexagonal-phase NaYF4:Yb,Er nanocrystals are the best NIR-to-visible up-converting materials to date, but YF3 phase due to its wider optical transmission window, and the minimization of the excited state quenching of doped ions, is considered to be promising material for application in biolabeling as well. In this work, biocompatible YF3:YbEr and GdF3:YbEr mesocrystals were prepared by one-pot solvothermal synthesis in the presence of chitosan, and are used successfully as biolabeling agents. The crystallinity and phase purity of the obtained powders were confirmed by X-ray powder diffraction which revealed that both samples are with the orthorhombic Pnma crystal structure, and monophase. Homogeneous distribution of dopant ions in the host matrix was confirmed by STEM/EDS mapping (not shown). SEM and TEM analysis implied that particles possess morphology similar to the unshelled peanuts, which are built from smaller monocrystals (Figs 1a and b). It was shown that their capacity toward self-organization is determined by the amount of surface-preserved chitosan ligands, which presence is confirmed by FTIR spectroscopy. Chitosan presence provides biocompatibility, thus the viability of both, MRC- 5 lung fibroblast, and A549 lung carcinoma cells was highly preserved (>80%), after 24 h of incubation with the synthetized samples (50μg mL-1). To monitor the intracellular uptake, cells incubated with particles were further analyzed by laser scanning microscopy under 980nm excitation. A strong up-conversion emission (green spots at Figs 1c and d), indicated that particles (green spots) are positioned in the cells cytoplasmic area adjacent to the plasma membrane, in both cell lines. Such positioning, without disturbing cells nuclei confirms their capacity to be used as NIR cell labeling agents.",
publisher = "Belgrade : Serbian Academy of Sciences and Arts",
journal = "Program & Book of Abstracts / Third International Conference ELMINA 2024, Belgrade, Serbia, September 9th -13th, 2024",
title = "Biocompatible Up-converting Yb/Er Doped Ln-fluoride Mesocrystals for Cell Labeling",
pages = "176-177",
url = "https://hdl.handle.net/21.15107/rcub_dais_16779"
}

Dinić, I., Vuković, M., Bukumira, M., Rabasović, M.,& Mančić, L.. (2024). Biocompatible Up-converting Yb/Er Doped Ln-fluoride Mesocrystals for Cell Labeling. in Program & Book of Abstracts / Third International Conference ELMINA 2024, Belgrade, Serbia, September 9th -13th, 2024
Belgrade : Serbian Academy of Sciences and Arts., 176-177.
https://hdl.handle.net/21.15107/rcub_dais_16779

Dinić I, Vuković M, Bukumira M, Rabasović M, Mančić L. Biocompatible Up-converting Yb/Er Doped Ln-fluoride Mesocrystals for Cell Labeling. in Program & Book of Abstracts / Third International Conference ELMINA 2024, Belgrade, Serbia, September 9th -13th, 2024. 2024;:176-177.
https://hdl.handle.net/21.15107/rcub_dais_16779 .

Dinić, Ivana, Vuković, Marina, Bukumira, Marta, Rabasović, Mihailo, Mančić, Lidija, "Biocompatible Up-converting Yb/Er Doped Ln-fluoride Mesocrystals for Cell Labeling" in Program & Book of Abstracts / Third International Conference ELMINA 2024, Belgrade, Serbia, September 9th -13th, 2024 (2024):176-177,
https://hdl.handle.net/21.15107/rcub_dais_16779 .

TY  - CONF
AU  - Piljević, Miljana
AU  - Dinić, Ivana
AU  - Bukumira, Marta
AU  - Rabasović, Mihailo
AU  - Krmpot, Aleksandar
AU  - Lazarević, Miloš
AU  - Mančić, Lidija
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16596
AB  - Cancer represents one of the leading problems of today, with clinical detection oftentimes being difficult, currently based on imaging techniques, such as X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). However, mortality rate is often reduced by early detection, therefore much focus has been directed towards developing methods for early detection of the disease.
Recent research in the field of nanotechnology is focused on the use of nanoparticles, particularly Lanthanide-doped up-conversion nanoparticles (UCNPs), for the detection of cancer cells using near infrared (NIR) fluorescence microscopy. The reason for this is that in long-term tracking tests, nearinfrared
(NIR) light, has lower phototoxicity and higher tissue penetration depth in living systems as compared with UV/VIS light. In this research, NaGd0.8Yb0.17Er0.03F4 UCNPs were prepared by solvothermal synthesis in the presence of chitosan, a ligand which enables UCNPs biocompatibility and the specific antibody conjugation. Morphological and structural characterization of synthetized UCNPs were performed based on X-ray powder diffraction (XRPD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and
photoluminescence spectroscopy (PL). Results confirmed the presence of the cubic phase with a minor portion of hexagonal phase in nanoparticles. Synthesized nanoparticles were conjugated further with anti-human CD44 antibodies, labeled with fluorescein isothiocyanate (FITC), which signal is used for confirmation of nanoparticles positioning in cells. Such obtained conjugates were successfully used for selective in vitro biolabeling of oral squamous cell carcinoma cells.
PB  - Belgrade : Institute of Physics
C3  - Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
T1  - Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles
SP  - 38
EP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_dais_16596
ER  - 

@conference{
author = "Piljević, Miljana and Dinić, Ivana and Bukumira, Marta and Rabasović, Mihailo and Krmpot, Aleksandar and Lazarević, Miloš and Mančić, Lidija",
year = "2024",
abstract = "Cancer represents one of the leading problems of today, with clinical detection oftentimes being difficult, currently based on imaging techniques, such as X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). However, mortality rate is often reduced by early detection, therefore much focus has been directed towards developing methods for early detection of the disease.
Recent research in the field of nanotechnology is focused on the use of nanoparticles, particularly Lanthanide-doped up-conversion nanoparticles (UCNPs), for the detection of cancer cells using near infrared (NIR) fluorescence microscopy. The reason for this is that in long-term tracking tests, nearinfrared
(NIR) light, has lower phototoxicity and higher tissue penetration depth in living systems as compared with UV/VIS light. In this research, NaGd0.8Yb0.17Er0.03F4 UCNPs were prepared by solvothermal synthesis in the presence of chitosan, a ligand which enables UCNPs biocompatibility and the specific antibody conjugation. Morphological and structural characterization of synthetized UCNPs were performed based on X-ray powder diffraction (XRPD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and
photoluminescence spectroscopy (PL). Results confirmed the presence of the cubic phase with a minor portion of hexagonal phase in nanoparticles. Synthesized nanoparticles were conjugated further with anti-human CD44 antibodies, labeled with fluorescein isothiocyanate (FITC), which signal is used for confirmation of nanoparticles positioning in cells. Such obtained conjugates were successfully used for selective in vitro biolabeling of oral squamous cell carcinoma cells.",
publisher = "Belgrade : Institute of Physics",
journal = "Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024",
title = "Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles",
pages = "38-38",
url = "https://hdl.handle.net/21.15107/rcub_dais_16596"
}

Piljević, M., Dinić, I., Bukumira, M., Rabasović, M., Krmpot, A., Lazarević, M.,& Mančić, L.. (2024). Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles. in Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
Belgrade : Institute of Physics., 38-38.
https://hdl.handle.net/21.15107/rcub_dais_16596

Piljević M, Dinić I, Bukumira M, Rabasović M, Krmpot A, Lazarević M, Mančić L. Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles. in Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024. 2024;:38-38.
https://hdl.handle.net/21.15107/rcub_dais_16596 .

Piljević, Miljana, Dinić, Ivana, Bukumira, Marta, Rabasović, Mihailo, Krmpot, Aleksandar, Lazarević, Miloš, Mančić, Lidija, "Selective in vitro labeling of cancer cells using NaGd0.8Yb0.17Er0.03F4 nanoparticles" in Book of Abstracts / 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024 (2024):38-38,
https://hdl.handle.net/21.15107/rcub_dais_16596 .

TY  - JOUR
AU  - Dinić, Ivana
AU  - Vuković, Marina
AU  - Rabanal, Maria Eugenia
AU  - Milošević, Milica
AU  - Bukumira, Marta
AU  - Tomić, Nina
AU  - Tomić, Miloš
AU  - Mančić, Lidija
AU  - Ignjatović, Nenad
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16211
AB  - Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation.
PB  - Basel : MDPI AG
T2  - Journal of Functional Biomaterials
T1  - Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals
SP  - 6
VL  - 15
IS  - 1
DO  - 10.3390/jfb15010006
UR  - https://hdl.handle.net/21.15107/rcub_dais_16211
ER  - 

@article{
author = "Dinić, Ivana and Vuković, Marina and Rabanal, Maria Eugenia and Milošević, Milica and Bukumira, Marta and Tomić, Nina and Tomić, Miloš and Mančić, Lidija and Ignjatović, Nenad",
year = "2024",
abstract = "Y0.8−xGdxF3:Yb/Er mesocrystals with a biocompatible surface and diverse morphological characteristics were successfully synthesized using chitosan-assisted solvothermal processing. Their structural properties, studied using X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning and transmission electron microscopy and energy dispersive X-ray analysis, were further correlated with the up-conversion emission (λexc = 976 nm) recorded in function of temperature. Based on the change in the visible green emissions originating from the thermally coupled 2H11/2 and 4S3/2 levels of Er3+, the corresponding LIR was acquired in the physiologically relevant range of temperatures (25–50 °C). The detected absolute sensitivity of about 0.0042 °C−1, along with the low cytotoxicity toward both normal human lung fibroblasts (MRC-5) and cancerous lung epithelial (A549) cells, indicate a potential for use in temperature sensing in biomedicine. Additionally, their enhanced internalization in cells, without suppression of cell viability, enabled in vitro labeling of cancer and healthy cells upon 976 nm laser irradiation.",
publisher = "Basel : MDPI AG",
journal = "Journal of Functional Biomaterials",
title = "Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals",
pages = "6",
volume = "15",
number = "1",
doi = "10.3390/jfb15010006",
url = "https://hdl.handle.net/21.15107/rcub_dais_16211"
}

Dinić, I., Vuković, M., Rabanal, M. E., Milošević, M., Bukumira, M., Tomić, N., Tomić, M., Mančić, L.,& Ignjatović, N.. (2024). Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals. in Journal of Functional Biomaterials
Basel : MDPI AG., 15(1), 6.
https://doi.org/10.3390/jfb15010006
https://hdl.handle.net/21.15107/rcub_dais_16211

Dinić I, Vuković M, Rabanal ME, Milošević M, Bukumira M, Tomić N, Tomić M, Mančić L, Ignjatović N. Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals. in Journal of Functional Biomaterials. 2024;15(1):6.
doi:10.3390/jfb15010006
https://hdl.handle.net/21.15107/rcub_dais_16211 .

Dinić, Ivana, Vuković, Marina, Rabanal, Maria Eugenia, Milošević, Milica, Bukumira, Marta, Tomić, Nina, Tomić, Miloš, Mančić, Lidija, Ignjatović, Nenad, "Temperature Sensing Properties of Biocompatible Yb/Er-Doped GdF3 and YF3 Mesocrystals" in Journal of Functional Biomaterials, 15, no. 1 (2024):6,
https://doi.org/10.3390/jfb15010006 .,
https://hdl.handle.net/21.15107/rcub_dais_16211 .

TY  - CONF
AU  - Tomić, Nina
AU  - Dinić, Ivana
AU  - Vuković, Marina
AU  - Bukumira, Marta
AU  - Rabasović, Mihailo
AU  - Mančić, Lidija
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/15155
AB  - There is a great technological interest in synthesis of lanthanide doped upconverting nanoparticles (UCNPs) with controlled crystal phase, morphology and intense luminescence properties suitable for biomedical use. A conventional approach for synthesis of such particles comprises decomposition of organometallic compounds in an oxygen-free environment, followed either with a ligand exchange, or biocompatible layer coating. Biocompatible NaY0.65Gd0.15F4:Yb,Er nanoparticles used in this study were synthesized through chitosan assisted one-pot hydrothermal synthesis and were characterized by X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDS) and photoluminescence measurement (PL). Due to the presence of the amino groups at their surface, excellent biocompatibility and notably low cytotoxicity against MRC-5 cells (line of normal human fibroblasts) and A549 cells (human lung cancer cells) were detected using MTT assay. Furthermore, upon 980 nm laser irradiation, particles were successfully used in vitro for labeling of both, MRC-5 and A549 cells.
PB  - Belgrade : Serbian Ceramic Society
C3  - Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023
T1  - One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_dais_15155
ER  - 

@conference{
author = "Tomić, Nina and Dinić, Ivana and Vuković, Marina and Bukumira, Marta and Rabasović, Mihailo and Mančić, Lidija",
year = "2023",
abstract = "There is a great technological interest in synthesis of lanthanide doped upconverting nanoparticles (UCNPs) with controlled crystal phase, morphology and intense luminescence properties suitable for biomedical use. A conventional approach for synthesis of such particles comprises decomposition of organometallic compounds in an oxygen-free environment, followed either with a ligand exchange, or biocompatible layer coating. Biocompatible NaY0.65Gd0.15F4:Yb,Er nanoparticles used in this study were synthesized through chitosan assisted one-pot hydrothermal synthesis and were characterized by X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDS) and photoluminescence measurement (PL). Due to the presence of the amino groups at their surface, excellent biocompatibility and notably low cytotoxicity against MRC-5 cells (line of normal human fibroblasts) and A549 cells (human lung cancer cells) were detected using MTT assay. Furthermore, upon 980 nm laser irradiation, particles were successfully used in vitro for labeling of both, MRC-5 and A549 cells.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023",
title = "One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_dais_15155"
}

Tomić, N., Dinić, I., Vuković, M., Bukumira, M., Rabasović, M.,& Mančić, L.. (2023). One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023
Belgrade : Serbian Ceramic Society., 70-70.
https://hdl.handle.net/21.15107/rcub_dais_15155

Tomić N, Dinić I, Vuković M, Bukumira M, Rabasović M, Mančić L. One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023. 2023;:70-70.
https://hdl.handle.net/21.15107/rcub_dais_15155 .

Tomić, Nina, Dinić, Ivana, Vuković, Marina, Bukumira, Marta, Rabasović, Mihailo, Mančić, Lidija, "One-Step Synthesis of Biocompatible NaY0.65Gd0.15F4:Yb,Er Upconverting Nanoparticles for In Vitro Cell Imaging" in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023 (2023):70-70,
https://hdl.handle.net/21.15107/rcub_dais_15155 .