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Tomić, Milica

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  • Tomić, Milica (1)
  • Tomić, Simonida (1)
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Author's Bibliography

Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro

Tomić, Milica; Stojanović, Sanja; Ignjatović, Nenad; Najman, Stevo

(Belgrade : Institute of Technical Sciences of SASA, 2019)

TY  - CONF
AU  - Tomić, Milica
AU  - Stojanović, Sanja
AU  - Ignjatović, Nenad
AU  - Najman, Stevo
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/6969
AB  - Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
T1  - Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro
SP  - 4
EP  - 4
ER  - 
@conference{
author = "Tomić, Milica and Stojanović, Sanja and Ignjatović, Nenad and Najman, Stevo",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/6969",
abstract = "Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia",
title = "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro",
pages = "4-4"
}
Tomić, M., Stojanović, S., Ignjatović, N.,& Najman, S. (2019). Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro.
Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, SerbiaBelgrade : Institute of Technical Sciences of SASA., 4-4.
Tomić M, Stojanović S, Ignjatović N, Najman S. Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro. Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia. 2019;:4-4
Tomić Milica, Stojanović Sanja, Ignjatović Nenad, Najman Stevo, "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro" (2019):4-4

Synthesis and properties of hydroxyapatite/poly-L-lactide composite biomaterials

Ignjatović, Nenad; Tomić, Simonida; Dakić, Momčilo; Miljković, Miroslav; Plavšić, Milenko; Uskoković, Dragan

(Oxford : Elsevier Sci Ltd, 1999)

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Tomić, Simonida
AU  - Dakić, Momčilo
AU  - Miljković, Miroslav
AU  - Plavšić, Milenko
AU  - Uskoković, Dragan
PY  - 1999
UR  - https://dais.sanu.ac.rs/123456789/10668
AB  - Calcium hydroxyapatite (HAp) and poly-L-lactide (PLLA) were synthesized chemically. The obtained HAp was of high purity and, after special thermal treatment, of high crystallinity as well. Synthesis of PLLA was performed using L-lactide as a monomer and nontoxic initiator. In this way a polymer of large molar weight (about 400 000) was obtained. The HAp and PLLA obtained were used as constituents of the HAp/PLLA composite biomaterial, a potential material for implants. The composite was obtained by mixing completely dissolved PLLA with granules of HAp. The composite was compacted by cold and hot pressing at pressures of 49.0-490.5 MPa and temperatures of 20-184 degrees C. The material obtained at optimum process parameters had a density of 99.6% and compressive strength of 93.2 MPa.
PB  - Oxford : Elsevier Sci Ltd
T2  - Biomaterials
T1  - Synthesis and properties of hydroxyapatite/poly-L-lactide composite biomaterials
SP  - 809
EP  - 816
VL  - 20
IS  - 9
DO  - 10.1016/S0142-9612(98)00234-8
ER  - 
@article{
author = "Ignjatović, Nenad and Tomić, Simonida and Dakić, Momčilo and Miljković, Miroslav and Plavšić, Milenko and Uskoković, Dragan",
year = "1999",
url = "https://dais.sanu.ac.rs/123456789/10668",
abstract = "Calcium hydroxyapatite (HAp) and poly-L-lactide (PLLA) were synthesized chemically. The obtained HAp was of high purity and, after special thermal treatment, of high crystallinity as well. Synthesis of PLLA was performed using L-lactide as a monomer and nontoxic initiator. In this way a polymer of large molar weight (about 400 000) was obtained. The HAp and PLLA obtained were used as constituents of the HAp/PLLA composite biomaterial, a potential material for implants. The composite was obtained by mixing completely dissolved PLLA with granules of HAp. The composite was compacted by cold and hot pressing at pressures of 49.0-490.5 MPa and temperatures of 20-184 degrees C. The material obtained at optimum process parameters had a density of 99.6% and compressive strength of 93.2 MPa.",
publisher = "Oxford : Elsevier Sci Ltd",
journal = "Biomaterials",
title = "Synthesis and properties of hydroxyapatite/poly-L-lactide composite biomaterials",
pages = "809-816",
volume = "20",
number = "9",
doi = "10.1016/S0142-9612(98)00234-8"
}
Ignjatović, N., Tomić, S., Dakić, M., Miljković, M., Plavšić, M.,& Uskoković, D. (1999). Synthesis and properties of hydroxyapatite/poly-L-lactide composite biomaterials.
BiomaterialsOxford : Elsevier Sci Ltd., 20(9), 809-816.
https://doi.org/10.1016/S0142-9612(98)00234-8
Ignjatović N, Tomić S, Dakić M, Miljković M, Plavšić M, Uskoković D. Synthesis and properties of hydroxyapatite/poly-L-lactide composite biomaterials. Biomaterials. 1999;20(9):809-816
Ignjatović Nenad, Tomić Simonida, Dakić Momčilo, Miljković Miroslav, Plavšić Milenko, Uskoković Dragan, "Synthesis and properties of hydroxyapatite/poly-L-lactide composite biomaterials" 20, no. 9 (1999):809-816,
https://doi.org/10.1016/S0142-9612(98)00234-8 .
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