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Stevanović, Magdalena

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Authority KeyName Variants
orcid::0000-0002-3989-0237
  • Stevanović, Magdalena (75)
  • Radić, Magdalena (1)
Projects
Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them Sinteza funkcionalnih materijala sa kontrolisanom strukturom na molekularnom i nano nivou
Italian Ministry of Foreign Affairs and International Cooperation (MAECI) within the collaboration framework between Italy and the Republic of Serbia (project PGR02952, call “Grande Rilevanza”) United States National Institutes of Health (NIH) / National Institute of Dental and Craniofacial Research (NIDCR), Grant K99-DE021416
Bilateral collaboration between Serbia and Slovenia (BI-RS/16-17-039) European Science Foundation COST Action CA15114
Microbial diversity study and characterization of beneficial environmental microorganisms Advanced technologies for monitoring and environmental protection from chemical pollutants and radiation burden
Slovenian Research Agency: Program P1-02456 Italian Ministry of University and Education (PRIN-2010 n. 2010B5B2NL)
Korean Institute of Science and Technology - Institute of Technical Science of SASA joint research project Advanced Materials for Biomedical Applications Ministry of Foreign Affairs and International Cooperation (Research Project of Particular Relevance between Italy and Serbia—PGR02952)
National Research Foundation of Korea - R11-2008-0061852 Serbian-German bilateral project no 451-03-01858/20 13-09/2 (DAAD project-ID 57060741)
Slovenian Research Agency: Program P1-0245 Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan
Examination of mechanisms of action, toxicity and interactions of adjuvant analgesics Italian Ministry of University and Education, PRIN-2010 n. 2010B5B2NL

Author's Bibliography

Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression

Ušjak, Dušan; Dinić, Miroslav; Novović, Katarina; Ivković, Branka; Filipović, Nenad; Stevanović, Magdalena; Milenković, Marina

(2020)

TY  - CONF
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3758
UR  - https://dais.sanu.ac.rs/123456789/10086
AB  - Acinetobacter baumannii je globalno rasprostranjen nozokomijalni patogen koji se odlikuje izuzetnom sposobnošću ekstremno brzog sticanja rezistencije na antibiotike, kao i adaptacije na preživljavanje u suvim uslovima bolničke sredine [1]. Zbog velike zastupljenosti rezistentnih sojeva protiv kojih ne postoji delotvorna terapija, Svetska zdravstvena organizacija (WHO, 2017) i Centri za kontrolu i prevenciju bolesti (CDC, 2019), označili su A. baumannii kao patogen od kritične važnosti za otkriće novih antimikrobnih agenasa ili novih terapijskih strategija [2]. Targetiranje virulencije je oblik alternativnog terapijskog pristupa koji pruža mogućnost prevencije teže kliničke slike kod inficiranih pacijenata posredstvom inhibicije ekspresije ključnih faktora virulencije, uz istovremenu redukovanu selekciju rezistentnih mutanata [3].Rezultati i Diskusija: Od četiri različito supstituisana hidroksihalkona, sintetisanih u postupku bazno-katalizovane Claisen-Schmidt kondenzacije, selektiran je metkosi-supstituisani derivat kao najpotentniji inhibitor produkcije biofilma kod A. baumannii. Primenom Real-Time kvantitativne PCR metode sa reverznom transkriptazom ispitan je uticaj subinhibitornih koncentracija selektiranog jedinjenja (70, 35 i 10 μg/mL) na ekspresiju gena faktora virulencije povezanih sa produkcijom biofilma kod A. baumannii: ompA, bap i abaI. Pokazana je značajna dozno-zavisna nishodna ekspresija ompA gena, koji kodira OmpA protein spoljašnje membrane ćelijskog zida, koji učestvuje u brojnim virulentnim osobinama A. baumannii, kao što su adhezija, citotoksičnost, motilitet i rezistencija na imunski odgovor i antibiotike [4]. Takođe, zabeležena je značajna inhibicija ekspresije bap gena, koja je neophodna za adheziju na humane epitelne ćelije, i abaI gena, integralnog dela bakterijskog kvorum-sensing sistema, koji kodira sintazu autoinduktorskih molekula. Sposobnost antivirulentnog delovanja metoksi-supstituisanog derivata hidroksihalkona potvrđena je demonstracijom inhibicije fenotipske ekspresije faktora virulencije povezanih sa ekspresijom ompA, bap i abaI gena, kao što su adhezija za komponente ekstracelularnog matriksa (fibronektin i kolagen), površinski motilitet i produkcija autoinduktorskih molekula.Zaključak: Metoksi-supstituisani hidroksihalkon ispoljava antivirulentno dejstvo protiv A. baumannii, pre svega posredstvom nishodne regulacije ompA gena, što se reflektuje u inhibiciji produkcije biofilma, sposobnosti adhezije i površinskog motiliteta ovog patogena.
AB  - Over the last two decades, Acinetobacter baumannii has emerged as one of the most troublesome pathogens, rapidly acquiring resistance to virtually all available antibiotics. This has urged researchers to seek alternative ways to fight this pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm activity of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. We used quantitative Real-Time PCR to evaluate mRNA expression of virulence-associated genes (ompA, bap, abaI) in extensively drug-resistant (XDR) A. baumannii wound isolate and A. baumannii ATCC 19606 strain, treated with selected compound. Also, we tested biofilm production, fibronectin- and collagen-mediated adhesion, surface motility and quorum-sensing activity of treated strains. The results revealed downregulation of the expression of all tested virulence genes together with the reduction of biofilm production, adhesion and motility. The most notable finding is significant reduction of ompA gene expression, whose encoded protein product is associated with numerous virulence traits of A. baumannii. Therefore, we conclude that selected methoxy-substituted hydroxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of the bacterial adhesins, most importantly OmpA, which is reflected in reduced biofilm formation, adhesion and surface motility.
C3  - FEMS Online Conference on Microbiology
T1  - Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression
ER  - 
@conference{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2020",
url = "https://farfar.pharmacy.bg.ac.rs/handle/123456789/3758, https://dais.sanu.ac.rs/123456789/10086",
abstract = "Acinetobacter baumannii je globalno rasprostranjen nozokomijalni patogen koji se odlikuje izuzetnom sposobnošću ekstremno brzog sticanja rezistencije na antibiotike, kao i adaptacije na preživljavanje u suvim uslovima bolničke sredine [1]. Zbog velike zastupljenosti rezistentnih sojeva protiv kojih ne postoji delotvorna terapija, Svetska zdravstvena organizacija (WHO, 2017) i Centri za kontrolu i prevenciju bolesti (CDC, 2019), označili su A. baumannii kao patogen od kritične važnosti za otkriće novih antimikrobnih agenasa ili novih terapijskih strategija [2]. Targetiranje virulencije je oblik alternativnog terapijskog pristupa koji pruža mogućnost prevencije teže kliničke slike kod inficiranih pacijenata posredstvom inhibicije ekspresije ključnih faktora virulencije, uz istovremenu redukovanu selekciju rezistentnih mutanata [3].Rezultati i Diskusija: Od četiri različito supstituisana hidroksihalkona, sintetisanih u postupku bazno-katalizovane Claisen-Schmidt kondenzacije, selektiran je metkosi-supstituisani derivat kao najpotentniji inhibitor produkcije biofilma kod A. baumannii. Primenom Real-Time kvantitativne PCR metode sa reverznom transkriptazom ispitan je uticaj subinhibitornih koncentracija selektiranog jedinjenja (70, 35 i 10 μg/mL) na ekspresiju gena faktora virulencije povezanih sa produkcijom biofilma kod A. baumannii: ompA, bap i abaI. Pokazana je značajna dozno-zavisna nishodna ekspresija ompA gena, koji kodira OmpA protein spoljašnje membrane ćelijskog zida, koji učestvuje u brojnim virulentnim osobinama A. baumannii, kao što su adhezija, citotoksičnost, motilitet i rezistencija na imunski odgovor i antibiotike [4]. Takođe, zabeležena je značajna inhibicija ekspresije bap gena, koja je neophodna za adheziju na humane epitelne ćelije, i abaI gena, integralnog dela bakterijskog kvorum-sensing sistema, koji kodira sintazu autoinduktorskih molekula. Sposobnost antivirulentnog delovanja metoksi-supstituisanog derivata hidroksihalkona potvrđena je demonstracijom inhibicije fenotipske ekspresije faktora virulencije povezanih sa ekspresijom ompA, bap i abaI gena, kao što su adhezija za komponente ekstracelularnog matriksa (fibronektin i kolagen), površinski motilitet i produkcija autoinduktorskih molekula.Zaključak: Metoksi-supstituisani hidroksihalkon ispoljava antivirulentno dejstvo protiv A. baumannii, pre svega posredstvom nishodne regulacije ompA gena, što se reflektuje u inhibiciji produkcije biofilma, sposobnosti adhezije i površinskog motiliteta ovog patogena., Over the last two decades, Acinetobacter baumannii has emerged as one of the most troublesome pathogens, rapidly acquiring resistance to virtually all available antibiotics. This has urged researchers to seek alternative ways to fight this pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm activity of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. We used quantitative Real-Time PCR to evaluate mRNA expression of virulence-associated genes (ompA, bap, abaI) in extensively drug-resistant (XDR) A. baumannii wound isolate and A. baumannii ATCC 19606 strain, treated with selected compound. Also, we tested biofilm production, fibronectin- and collagen-mediated adhesion, surface motility and quorum-sensing activity of treated strains. The results revealed downregulation of the expression of all tested virulence genes together with the reduction of biofilm production, adhesion and motility. The most notable finding is significant reduction of ompA gene expression, whose encoded protein product is associated with numerous virulence traits of A. baumannii. Therefore, we conclude that selected methoxy-substituted hydroxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of the bacterial adhesins, most importantly OmpA, which is reflected in reduced biofilm formation, adhesion and surface motility.",
journal = "FEMS Online Conference on Microbiology",
title = "Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression"
}
Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M. (2020). Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression.
FEMS Online Conference on Microbiology.
Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković M. Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression. FEMS Online Conference on Microbiology. 2020;
Ušjak Dušan, Dinić Miroslav, Novović Katarina, Ivković Branka, Filipović Nenad, Stevanović Magdalena, Milenković Marina, "Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression" (2020)

Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression

Ušjak, Dušan; Dinić, Miroslav; Novović, Katarina; Ivković, Branka; Filipović, Nenad; Stevanović, Magdalena; Milenković, Marina T.

(Wiley, 2020)

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina T.
PY  - 2020
UR  - https://dais.sanu.ac.rs/123456789/10034
AB  - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
PB  - Wiley
T2  - Chemistry & Biodiversity
T1  - Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression
DO  - 10.1002/cbdv.202000786
ER  - 
@article{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina T.",
year = "2020",
url = "https://dais.sanu.ac.rs/123456789/10034",
abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real‐time PCR was used to evaluate mRNA expression of biofilm‐associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin‐ and collagen‐mediated adhesion, surface motility, and quorum‐sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′‐hydroxy‐2‐methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.",
publisher = "Wiley",
journal = "Chemistry & Biodiversity",
title = "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression",
doi = "10.1002/cbdv.202000786"
}
Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M. T. (2020). Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression.
Chemistry & BiodiversityWiley..
https://doi.org/10.1002/cbdv.202000786
Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković MT. Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression. Chemistry & Biodiversity. 2020;
Ušjak Dušan, Dinić Miroslav, Novović Katarina, Ivković Branka, Filipović Nenad, Stevanović Magdalena, Milenković Marina T., "Methoxy‐Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of                    Acinetobacter baumannii                    by Inhibiting                    ompA                    Gene Expression" (2020),
https://doi.org/10.1002/cbdv.202000786 .
3

Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging

Catanzaro, Valeria; Digilio, Giuseppe; Capuana, Federico; Padovan, Sergio; Cutrin, Juan C.; Carniato, Fabio; Porta, Stefano; Grange, Cristina; Filipović, Nenad; Stevanović, Magdalena

(Basel : MDPI, 2019)

TY  - BOOK
AU  - Catanzaro, Valeria
AU  - Digilio, Giuseppe
AU  - Capuana, Federico
AU  - Padovan, Sergio
AU  - Cutrin, Juan C.
AU  - Carniato, Fabio
AU  - Porta, Stefano
AU  - Grange, Cristina
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/7044
AB  - Table S1. List of the antibodies used in this study; Figure S1 Transmission electron micrographs of particle sections, showing electron dense Gd-NPs with diameter of 1-2 nm; Figure S2 Optical images at the inverted microscope, showing hMSCs after 3 days seeding with ILCSs. The arrows show hMSCs on the particle surface (A) or at the junction between particles (B, C, D); Figure S3 SEM micrographs of ILCSs seeded with hMSCs (after 10 days culture) at (A) 500x and (B) 200x magnification. Cells have been fixed with formalin for SEM. Cells appear mostly located at the junction between adjacent microparticles; Figure S4 Assessment of the multipotentiality of hMSCs after incubation up to 20 days with ILCS. A) Multipotentiality markers by flow cytometry analysis; B) Differentiation into adipocytes (middle, Oil Red staining) or osteocytes (right, Alizarin Red staining). The left panel is the control; Figure S5 Expansions of MR images around the ̶ hMSCs grafts (contralateral to the implants shown in Fig. 5, main text) in an immunocompromised NSG mouse (ad) and an immunocompetent FVB mouse (e-h). Similar to +hMSCs implants, activation of contrast enhancement in T1w-MR images is observed in the immunocompromised mouse on going from day-0 (b) to day-12 (d). Poor activation of contrast enhancement is observed for the immunocompetent mouse (f,h); Figure S6 Photograph of the Matrigel-based hydrogel embedding cell-loaded ILCSs (pink spots) excised from an immunocompromised mouse 20 days after implantation; Figure S7 Histology of -hMSC subcutaneous cell implants excised from a representative immunocompromised NSG mouse (a-c) and immunocompetent FVB mouse (df). (a,d) H&E stains; (b,e) Masson stains; (c,f) Sirius red stains. Arrows indicate microspheres delimited by an intense fibrotic reaction. Arrow-heads are pointing the vascular organization of the matrigel. Double arrows are indicating macrophage foamy cells. Scale bar: 50 μm for a,b,d,e; 25 μm for c,f; Figure S8 Schematics about the geometry of MRI slices across ILCS implants to measure the signal enhancement (see main text, Section 4.5.2.)
PB  - Basel : MDPI
T2  - Journal of Functional Biomaterials
T1  - Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging
VL  - 10
IS  - 3
ER  - 
@book{
author = "Catanzaro, Valeria and Digilio, Giuseppe and Capuana, Federico and Padovan, Sergio and Cutrin, Juan C. and Carniato, Fabio and Porta, Stefano and Grange, Cristina and Filipović, Nenad and Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/7044",
abstract = "Table S1. List of the antibodies used in this study; Figure S1 Transmission electron micrographs of particle sections, showing electron dense Gd-NPs with diameter of 1-2 nm; Figure S2 Optical images at the inverted microscope, showing hMSCs after 3 days seeding with ILCSs. The arrows show hMSCs on the particle surface (A) or at the junction between particles (B, C, D); Figure S3 SEM micrographs of ILCSs seeded with hMSCs (after 10 days culture) at (A) 500x and (B) 200x magnification. Cells have been fixed with formalin for SEM. Cells appear mostly located at the junction between adjacent microparticles; Figure S4 Assessment of the multipotentiality of hMSCs after incubation up to 20 days with ILCS. A) Multipotentiality markers by flow cytometry analysis; B) Differentiation into adipocytes (middle, Oil Red staining) or osteocytes (right, Alizarin Red staining). The left panel is the control; Figure S5 Expansions of MR images around the ̶ hMSCs grafts (contralateral to the implants shown in Fig. 5, main text) in an immunocompromised NSG mouse (ad) and an immunocompetent FVB mouse (e-h). Similar to +hMSCs implants, activation of contrast enhancement in T1w-MR images is observed in the immunocompromised mouse on going from day-0 (b) to day-12 (d). Poor activation of contrast enhancement is observed for the immunocompetent mouse (f,h); Figure S6 Photograph of the Matrigel-based hydrogel embedding cell-loaded ILCSs (pink spots) excised from an immunocompromised mouse 20 days after implantation; Figure S7 Histology of -hMSC subcutaneous cell implants excised from a representative immunocompromised NSG mouse (a-c) and immunocompetent FVB mouse (df). (a,d) H&E stains; (b,e) Masson stains; (c,f) Sirius red stains. Arrows indicate microspheres delimited by an intense fibrotic reaction. Arrow-heads are pointing the vascular organization of the matrigel. Double arrows are indicating macrophage foamy cells. Scale bar: 50 μm for a,b,d,e; 25 μm for c,f; Figure S8 Schematics about the geometry of MRI slices across ILCS implants to measure the signal enhancement (see main text, Section 4.5.2.)",
publisher = "Basel : MDPI",
journal = "Journal of Functional Biomaterials",
title = "Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging",
volume = "10",
number = "3"
}
Catanzaro, V., Digilio, G., Capuana, F., Padovan, S., Cutrin, J. C., Carniato, F., Porta, S., Grange, C., Filipović, N.,& Stevanović, M. (2019). Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging.
Journal of Functional BiomaterialsBasel : MDPI., 10(3).
Catanzaro V, Digilio G, Capuana F, Padovan S, Cutrin JC, Carniato F, Porta S, Grange C, Filipović N, Stevanović M. Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. Journal of Functional Biomaterials. 2019;10(3)
Catanzaro Valeria, Digilio Giuseppe, Capuana Federico, Padovan Sergio, Cutrin Juan C., Carniato Fabio, Porta Stefano, Grange Cristina, Filipović Nenad, Stevanović Magdalena, "Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging" 10, no. 3 (2019)

Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073

Filipović, Nenad; Veselinović, Ljiljana; Ražić, Slavica; Jeremić, Sanja; Filipič, Metka; Žegura, Bojana; Tomić, Sergej; Čolić, Miodrag; Stevanović, Magdalena

(2019)

TY  - BOOK
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/5972
T2  - Materials Science and Engineering C
T1  - Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073
ER  - 
@book{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/5972",
journal = "Materials Science and Engineering C",
title = "Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073"
}
Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M.,& Stevanović, M. (2019). Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073.
Materials Science and Engineering C.
Filipović N, Veselinović L, Ražić S, Jeremić S, Filipič M, Žegura B, Tomić S, Čolić M, Stevanović M. Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073. Materials Science and Engineering C. 2019;
Filipović Nenad, Veselinović Ljiljana, Ražić Slavica, Jeremić Sanja, Filipič Metka, Žegura Bojana, Tomić Sergej, Čolić Miodrag, Stevanović Magdalena, "Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073" (2019)

Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging

Catanzaro, Valeria; Digilio, Giuseppe; Capuana, Federico; Padovan, Sergio; Cutrin, Juan C.; Carniato, Fabio; Porta, Stefano; Grange, Cristina; Filipović, Nenad; Stevanović, Magdalena

(Basel : MDPI, 2019)

TY  - JOUR
AU  - Catanzaro, Valeria
AU  - Digilio, Giuseppe
AU  - Capuana, Federico
AU  - Padovan, Sergio
AU  - Cutrin, Juan C.
AU  - Carniato, Fabio
AU  - Porta, Stefano
AU  - Grange, Cristina
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/6689
AB  - Cell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200–600 µm diameter and 50–80 µm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T1-weighted (T1w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progressive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs. © 2019 by the authors.
PB  - Basel : MDPI
T2  - Journal of Functional Biomaterials
T1  - Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging
SP  - 28
VL  - 10
IS  - 3
DO  - 10.3390/jfb10030028
ER  - 
@article{
author = "Catanzaro, Valeria and Digilio, Giuseppe and Capuana, Federico and Padovan, Sergio and Cutrin, Juan C. and Carniato, Fabio and Porta, Stefano and Grange, Cristina and Filipović, Nenad and Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/6689",
abstract = "Cell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200–600 µm diameter and 50–80 µm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T1-weighted (T1w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progressive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs. © 2019 by the authors.",
publisher = "Basel : MDPI",
journal = "Journal of Functional Biomaterials",
title = "Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging",
pages = "28",
volume = "10",
number = "3",
doi = "10.3390/jfb10030028"
}
Catanzaro, V., Digilio, G., Capuana, F., Padovan, S., Cutrin, J. C., Carniato, F., Porta, S., Grange, C., Filipović, N.,& Stevanović, M. (2019). Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging.
Journal of Functional BiomaterialsBasel : MDPI., 10(3), 28.
https://doi.org/10.3390/jfb10030028
Catanzaro V, Digilio G, Capuana F, Padovan S, Cutrin JC, Carniato F, Porta S, Grange C, Filipović N, Stevanović M. Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. Journal of Functional Biomaterials. 2019;10(3):28
Catanzaro Valeria, Digilio Giuseppe, Capuana Federico, Padovan Sergio, Cutrin Juan C., Carniato Fabio, Porta Stefano, Grange Cristina, Filipović Nenad, Stevanović Magdalena, "Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging" 10, no. 3 (2019):28,
https://doi.org/10.3390/jfb10030028 .
1
2
2

Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles

Filipović, Nenad; Veselinović, Ljiljana; Ražić, Slavica; Jeremić, Sanja; Filipič, Metka; Žegura, Bojana; Tomić, Sergej; Čolić, Miodrag; Stevanović, Magdalena

(Elsevier, 2019)

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/4600
AB  - Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles
SP  - 776
EP  - 789
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
ER  - 
@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/4600",
abstract = "Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "776-789",
volume = "96",
doi = "10.1016/j.msec.2018.11.073"
}
Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M.,& Stevanović, M. (2019). Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles.
Materials Science and Engineering CElsevier., 96, 776-789.
https://doi.org/10.1016/j.msec.2018.11.073
Filipović N, Veselinović L, Ražić S, Jeremić S, Filipič M, Žegura B, Tomić S, Čolić M, Stevanović M. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C. 2019;96:776-789
Filipović Nenad, Veselinović Ljiljana, Ražić Slavica, Jeremić Sanja, Filipič Metka, Žegura Bojana, Tomić Sergej, Čolić Miodrag, Stevanović Magdalena, "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles" 96 (2019):776-789,
https://doi.org/10.1016/j.msec.2018.11.073 .
1
5
8
8

Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles

Filipović, Nenad; Veselinović, Ljiljana; Ražić, Slavica; Jeremić, Sanja; Filipič, Metka; Žegura, Bojana; Tomić, Sergej; Čolić, Miodrag; Stevanović, Magdalena

(Elsevier, 2019)

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/4590
AB  - Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles
SP  - 776
EP  - 789
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
ER  - 
@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/4590",
abstract = "Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "776-789",
volume = "96",
doi = "10.1016/j.msec.2018.11.073"
}
Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M.,& Stevanović, M. (2019). Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles.
Materials Science and Engineering CElsevier., 96, 776-789.
https://doi.org/10.1016/j.msec.2018.11.073
Filipović N, Veselinović L, Ražić S, Jeremić S, Filipič M, Žegura B, Tomić S, Čolić M, Stevanović M. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C. 2019;96:776-789
Filipović Nenad, Veselinović Ljiljana, Ražić Slavica, Jeremić Sanja, Filipič Metka, Žegura Bojana, Tomić Sergej, Čolić Miodrag, Stevanović Magdalena, "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles" 96 (2019):776-789,
https://doi.org/10.1016/j.msec.2018.11.073 .
1
5
8
8

Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives

Stevanović, Magdalena; Lukić, Miodrag J.; Stanković, Ana; Filipović, Nenad; Kuzmanović, Maja; Janićijević, Željko

(Elsevier, 2019)

TY  - CHAP
AU  - Stevanović, Magdalena
AU  - Lukić, Miodrag J.
AU  - Stanković, Ana
AU  - Filipović, Nenad
AU  - Kuzmanović, Maja
AU  - Janićijević, Željko
PY  - 2019
UR  - http://www.sciencedirect.com/science/article/pii/B9780081028148000019
UR  - http://dais.sanu.ac.rs/123456789/5760
AB  - Nanotechnology has great potential in the biomedical field. Among other nanomaterials, inorganic nanoparticles have become extremely important since they possess unique physicochemical properties influenced by their specific surface structure. Consequently, inorganic nanoparticles exhibit enhanced functionalities such as biological response, antibacterial and antiviral properties, as well as optical, magnetic, and electrical responses. They have found applications in medicine, pharmacy, controlled drug delivery, optics, electronics, etc. In this chapter, reports on obtaining different metallic and ceramic inorganic nanoparticles such as gold, silver, selenium, copper, iron, zinc oxide, and hydroxyapatite for biomedical applications will be addressed. For each of these nanosystems, the main challenges regarding the currently achieved functional properties and further perspectives will also be presented.
PB  - Elsevier
T2  - Materials for Biomedical Engineering
T1  - Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives
SP  - 1
EP  - 46
DO  - 10.1016/B978-0-08-102814-8.00001-9
ER  - 
@article{
author = "Stevanović, Magdalena and Lukić, Miodrag J. and Stanković, Ana and Filipović, Nenad and Kuzmanović, Maja and Janićijević, Željko",
year = "2019",
url = "http://www.sciencedirect.com/science/article/pii/B9780081028148000019, http://dais.sanu.ac.rs/123456789/5760",
abstract = "Nanotechnology has great potential in the biomedical field. Among other nanomaterials, inorganic nanoparticles have become extremely important since they possess unique physicochemical properties influenced by their specific surface structure. Consequently, inorganic nanoparticles exhibit enhanced functionalities such as biological response, antibacterial and antiviral properties, as well as optical, magnetic, and electrical responses. They have found applications in medicine, pharmacy, controlled drug delivery, optics, electronics, etc. In this chapter, reports on obtaining different metallic and ceramic inorganic nanoparticles such as gold, silver, selenium, copper, iron, zinc oxide, and hydroxyapatite for biomedical applications will be addressed. For each of these nanosystems, the main challenges regarding the currently achieved functional properties and further perspectives will also be presented.",
publisher = "Elsevier",
journal = "Materials for Biomedical Engineering",
title = "Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives",
pages = "1-46",
doi = "10.1016/B978-0-08-102814-8.00001-9"
}
Stevanović, M., Lukić, M. J., Stanković, A., Filipović, N., Kuzmanović, M.,& Janićijević, Ž. (2019). Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives.
Materials for Biomedical EngineeringElsevier., 1-46.
https://doi.org/10.1016/B978-0-08-102814-8.00001-9
Stevanović M, Lukić MJ, Stanković A, Filipović N, Kuzmanović M, Janićijević Ž. Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives. Materials for Biomedical Engineering. 2019;:1-46
Stevanović Magdalena, Lukić Miodrag J., Stanković Ana, Filipović Nenad, Kuzmanović Maja, Janićijević Željko, "Chapter 1 - Biomedical inorganic nanoparticles: preparation, properties, and perspectives" (2019):1-46,
https://doi.org/10.1016/B978-0-08-102814-8.00001-9 .
1
2

Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles

Janićijević, Željko; Stanković, Ana; Žegura, Bojana; Veljović, Đorđe; Filipič, Metka; Stevanović, Magdalena

(Belgrade : Institute of Technical Sciences of SASA, 2019)

TY  - CONF
AU  - Janićijević, Željko
AU  - Stanković, Ana
AU  - Žegura, Bojana
AU  - Veljović, Đorđe
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/6968
AB  - Nanostructured zinc oxides are promising materials for numerous biomedical applications where they can serve as therapeutic agents or tools for sensing and imaging. Despite their favorable properties, wider use of zinc oxide nanoparticles in biomedicine is limited by toxicity issues. Therefore, new synthesis approaches should be devised to obtain zinc oxide nanoparticles which are safe-by-design. We present an innovative low-cost wet precipitation synthesis of gelatin modified zinc oxide nanoparticles at the gel/liquid interface. The diffusion of ammonia through the gelatin hydrogels of different porosities induces precipitation of the product in contact with the surface of the aqueous solution of zinc ions. After thermal treatment of the precipitate, adsorbed organic residues of decomposed gelatin act as modifiers of zinc oxide nanoparticles. We characterized the physicochemical properties of obtained gelatin modified zinc oxide nanoparticles by XRD, FTIR, DTA/TG, and SEM. The synthesized nanoparticles show hexagonal wurtzite structure and form flakelike aggregates. FTIR and DTA/TG analyses indicate that the thermal decomposition of adsorbed gelatin depends on the gelatin content of the hydrogel used in the synthesis. We also examined the viability of HepG2 cells, generation of intracellular reactive oxygen species, and genotoxicity using the MTS, DCFH-DA, and alkaline comet assay, respectively. Fabricated gelatin modified zinc oxide nanoparticles show very low toxicity potential at doses relevant for human exposure.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia
T1  - Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles
SP  - 3
EP  - 3
ER  - 
@conference{
author = "Janićijević, Željko and Stanković, Ana and Žegura, Bojana and Veljović, Đorđe and Filipič, Metka and Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/6968",
abstract = "Nanostructured zinc oxides are promising materials for numerous biomedical applications where they can serve as therapeutic agents or tools for sensing and imaging. Despite their favorable properties, wider use of zinc oxide nanoparticles in biomedicine is limited by toxicity issues. Therefore, new synthesis approaches should be devised to obtain zinc oxide nanoparticles which are safe-by-design. We present an innovative low-cost wet precipitation synthesis of gelatin modified zinc oxide nanoparticles at the gel/liquid interface. The diffusion of ammonia through the gelatin hydrogels of different porosities induces precipitation of the product in contact with the surface of the aqueous solution of zinc ions. After thermal treatment of the precipitate, adsorbed organic residues of decomposed gelatin act as modifiers of zinc oxide nanoparticles. We characterized the physicochemical properties of obtained gelatin modified zinc oxide nanoparticles by XRD, FTIR, DTA/TG, and SEM. The synthesized nanoparticles show hexagonal wurtzite structure and form flakelike aggregates. FTIR and DTA/TG analyses indicate that the thermal decomposition of adsorbed gelatin depends on the gelatin content of the hydrogel used in the synthesis. We also examined the viability of HepG2 cells, generation of intracellular reactive oxygen species, and genotoxicity using the MTS, DCFH-DA, and alkaline comet assay, respectively. Fabricated gelatin modified zinc oxide nanoparticles show very low toxicity potential at doses relevant for human exposure.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia",
title = "Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles",
pages = "3-3"
}
Janićijević, Ž., Stanković, A., Žegura, B., Veljović, Đ., Filipič, M.,& Stevanović, M. (2019). Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles.
Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, SerbiaBelgrade : Institute of Technical Sciences of SASA., 3-3.
Janićijević Ž, Stanković A, Žegura B, Veljović Đ, Filipič M, Stevanović M. Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles. Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia. 2019;:3-3
Janićijević Željko, Stanković Ana, Žegura Bojana, Veljović Đorđe, Filipič Metka, Stevanović Magdalena, "Synthesis, characterization and toxicity studies of gelatin modified zinc oxide nanoparticles" (2019):3-3

Chapter 1: Biomedical Applications of Nanostructured Polymeric Materials

Swain, Sarat Kumar; Jawaid, Mohammad; Stevanović, Magdalena

(Elsevier, 2019)

TY  - CHAP
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://www.sciencedirect.com/science/article/pii/B9780128167717000016
UR  - http://dais.sanu.ac.rs/123456789/6946
AB  - Nanostructured polymeric materials have been extensively used in different areas of biomedicine. They are polymeric materials in nanometer scale or composites containing polymeric nanomaterials. In particular, improvements in polymer-correlated nanomaterials have brought about a groundbreaking change to the fields of biomedicine and innovative biomaterials. The materials which belong to this group of nanostructured polymeric materials are a wide variety of nanoparticles, nanocapsules, nanogels, nanofibers, nanocomposites, micelles, dendrimers, and polymersomes. They can be used in controlled and targeted drug delivery, bioimaging, tissue engineering, and regenerative medicine. This chapter addresses issues regarding nanostructured polymeric materials, their production methods, characterizations, and biomedical applications.
PB  - Elsevier
T2  - Nanostructured Polymer Composites for Biomedical Applications
T1  - Chapter 1: Biomedical Applications of Nanostructured Polymeric Materials
SP  - 1
EP  - 19
DO  - 10.1016/B978-0-12-816771-7.00001-6
ER  - 
@article{
editor = "Swain, Sarat Kumar, Jawaid, Mohammad",
author = "Stevanović, Magdalena",
year = "2019",
url = "http://www.sciencedirect.com/science/article/pii/B9780128167717000016, http://dais.sanu.ac.rs/123456789/6946",
abstract = "Nanostructured polymeric materials have been extensively used in different areas of biomedicine. They are polymeric materials in nanometer scale or composites containing polymeric nanomaterials. In particular, improvements in polymer-correlated nanomaterials have brought about a groundbreaking change to the fields of biomedicine and innovative biomaterials. The materials which belong to this group of nanostructured polymeric materials are a wide variety of nanoparticles, nanocapsules, nanogels, nanofibers, nanocomposites, micelles, dendrimers, and polymersomes. They can be used in controlled and targeted drug delivery, bioimaging, tissue engineering, and regenerative medicine. This chapter addresses issues regarding nanostructured polymeric materials, their production methods, characterizations, and biomedical applications.",
publisher = "Elsevier",
journal = "Nanostructured Polymer Composites for Biomedical Applications",
title = "Chapter 1: Biomedical Applications of Nanostructured Polymeric Materials",
pages = "1-19",
doi = "10.1016/B978-0-12-816771-7.00001-6"
}
Swain, S. K., Jawaid, M.,& Stevanović, M. (2019). Chapter 1: Biomedical Applications of Nanostructured Polymeric Materials.
Nanostructured Polymer Composites for Biomedical ApplicationsElsevier., 1-19.
https://doi.org/10.1016/B978-0-12-816771-7.00001-6
Swain SK, Jawaid M, Stevanović M. Chapter 1: Biomedical Applications of Nanostructured Polymeric Materials. Nanostructured Polymer Composites for Biomedical Applications. 2019;:1-19
Swain Sarat Kumar, Jawaid Mohammad, Stevanović Magdalena, "Chapter 1: Biomedical Applications of Nanostructured Polymeric Materials" (2019):1-19,
https://doi.org/10.1016/B978-0-12-816771-7.00001-6 .
2

Polyester micro- and nanosized systems with therapeutic functionality

Stevanović, Magdalena

(ENIUS, 2019)

TY  - CONF
AU  - Stevanović, Magdalena
PY  - 2019
UR  - http://dais.sanu.ac.rs/123456789/5274
AB  - Among the families of synthetic polymers, polyesters are very attractive for various medical, pharmaceutical, industrial and other purposes. The major applications include drug delivery systems, tissue engineering applications, resorbable sutures, and orthopaedic fixation devices. Polyester, poly (lactide-co-glycolide) (PLGA) is a copolymer of poly lactic acid (PLA) and poly glycolic acid (PGA). PLGA is biocompatible and biodegradable copolymer whit tunable properties. It is approved by FDA and has been comprehensively studied for the development of different systems and materials for commercial use and in research. It is often used for preparing controlled drug delivery devices. PLGA polymer particles allow the encapsulation of the medicament within the polymer matrix, where the basic requirement for the controlled and balanced release of the medicament in the body is its ideal spherical shape and narrow distribution of their sizes. Until now, different active substances have been successfully encapsulated within PLGA polymer matrix thus creating micro or nanosized systems with sustained release and different functionalities. One example is simultaneously encapsulation of water-soluble antioxidant (ascorbic acid, vitamin C) and poly(L-glutamic acid)-capped silver nanoparticles (AgNpPGA), within PLGA particles. These PLGA/AgNpPGA/ascorbic acid microspheres have been examined in terms of structural characteristics, morphology, stability, in vitro degradation, antimicrobial activity, cytotoxicity and induction of intracellular reactive oxygen species. They have demonstrated synergistic effects i.e. effective antioxidative and, at the same time, prolonged antimicrobial activity.
PB  - ENIUS
C3  - Conference proceedings / ENIUS Workshop “Materials, Technology, and Biomimetics as enabling tools for a new generation of Urinary Stents” CA16217, Sofia, Bulgaria 31st January – 2nd February 2019
T1  - Polyester micro- and nanosized systems with therapeutic functionality
SP  - 51
EP  - 61
ER  - 
@conference{
author = "Stevanović, Magdalena",
year = "2019",
url = "http://dais.sanu.ac.rs/123456789/5274",
abstract = "Among the families of synthetic polymers, polyesters are very attractive for various medical, pharmaceutical, industrial and other purposes. The major applications include drug delivery systems, tissue engineering applications, resorbable sutures, and orthopaedic fixation devices. Polyester, poly (lactide-co-glycolide) (PLGA) is a copolymer of poly lactic acid (PLA) and poly glycolic acid (PGA). PLGA is biocompatible and biodegradable copolymer whit tunable properties. It is approved by FDA and has been comprehensively studied for the development of different systems and materials for commercial use and in research. It is often used for preparing controlled drug delivery devices. PLGA polymer particles allow the encapsulation of the medicament within the polymer matrix, where the basic requirement for the controlled and balanced release of the medicament in the body is its ideal spherical shape and narrow distribution of their sizes. Until now, different active substances have been successfully encapsulated within PLGA polymer matrix thus creating micro or nanosized systems with sustained release and different functionalities. One example is simultaneously encapsulation of water-soluble antioxidant (ascorbic acid, vitamin C) and poly(L-glutamic acid)-capped silver nanoparticles (AgNpPGA), within PLGA particles. These PLGA/AgNpPGA/ascorbic acid microspheres have been examined in terms of structural characteristics, morphology, stability, in vitro degradation, antimicrobial activity, cytotoxicity and induction of intracellular reactive oxygen species. They have demonstrated synergistic effects i.e. effective antioxidative and, at the same time, prolonged antimicrobial activity.",
publisher = "ENIUS",
journal = "Conference proceedings / ENIUS Workshop “Materials, Technology, and Biomimetics as enabling tools for a new generation of Urinary Stents” CA16217, Sofia, Bulgaria 31st January – 2nd February 2019",
title = "Polyester micro- and nanosized systems with therapeutic functionality",
pages = "51-61"
}
Stevanović, M. (2019). Polyester micro- and nanosized systems with therapeutic functionality.
Conference proceedings / ENIUS Workshop “Materials, Technology, and Biomimetics as enabling tools for a new generation of Urinary Stents” CA16217, Sofia, Bulgaria 31st January – 2nd February 2019ENIUS., 51-61.
Stevanović M. Polyester micro- and nanosized systems with therapeutic functionality. Conference proceedings / ENIUS Workshop “Materials, Technology, and Biomimetics as enabling tools for a new generation of Urinary Stents” CA16217, Sofia, Bulgaria 31st January – 2nd February 2019. 2019;:51-61
Stevanović Magdalena, "Polyester micro- and nanosized systems with therapeutic functionality" (2019):51-61

Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels

Padovan, Sergio; Catanzaro, Valeria; Capuana, Federico; Grange, Cristina; Koni, Malvina; Carrera, Carla; Filipović, Nenad; Stevanović, Magdalena

(2019)

TY  - CONF
AU  - Padovan, Sergio
AU  - Catanzaro, Valeria
AU  - Capuana, Federico
AU  - Grange, Cristina
AU  - Koni, Malvina
AU  - Carrera, Carla
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://eventclass.org/contxt_emim2019/online-program/session?s=PS+22#e83
UR  - http://dais.sanu.ac.rs/123456789/5251
AB  - *Introduction*In regenerative medicine, biocompatible hydrogels are increasingly used to encapsulate therapeutic cells prior to transplantation into the host to enhance their long term survival. Cell embedding within bioengineered hydrogels can shield cells from immune response and provide an optimal life-sustaining microenvironment to therapeutic cells. In addition, cell embedding offers the outstanding opportunity to insert microenvironment-responsive imaging labels within the hydrogel, paving the way for non-invasive monitoring of the extracellular microenvironment within the hydrogel. We have inserted redox-responsive MRI labels within cell-embedding hydrogels to follow-up the microenvironment redox state.*Methods*High molecular weight chitosan polymers were chemically conjugated with a Gd-HPDO3A-chelate through a disulfide bond, and interspersed within alginate-based hydrogel capsules. Human mesenchymal stem cells (hMSCs) as model therapeutic cells were embedded into such imaging labelled hydrogel. Embedded cells were incubated under simulated hypoxiaconditions, while being followed-up by T1-weighted MRI at 7T.*Results*Under reducing conditions, reductive cleavage of the disulfide bond in the Gd-chitosan probe yields a low molecular weight Gd-chelate that eventually diffuses out of the hydrogel capsule. The resulting change of MRI contrast enhancement along time is very sensitive to the oxygenation level within cell capsules. The kinetics of clearance of contrast enhancement is an indirect indicator of the survival of encapsulated cells.*Conclusions*The Gd-chitosan probe we developed is promising to follow-up non-invasively the redox microenvironment within cellembedding hydrogels. This approach will find useful application to monitor whether transplanted cells succeed to restore normal tissue oxygenation levels, especially in regenerative medicine approaches to ischemic diseases.
C3  - European Molecular Imaging Meeting - EMIM 2019, March 19-22, 2019, Scottish Event Campus - SEC, Glasgow, UK: Online Program
T1  - Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels
ER  - 
@conference{
author = "Padovan, Sergio and Catanzaro, Valeria and Capuana, Federico and Grange, Cristina and Koni, Malvina and Carrera, Carla and Filipović, Nenad and Stevanović, Magdalena",
year = "2019",
url = "https://eventclass.org/contxt_emim2019/online-program/session?s=PS+22#e83, http://dais.sanu.ac.rs/123456789/5251",
abstract = "*Introduction*In regenerative medicine, biocompatible hydrogels are increasingly used to encapsulate therapeutic cells prior to transplantation into the host to enhance their long term survival. Cell embedding within bioengineered hydrogels can shield cells from immune response and provide an optimal life-sustaining microenvironment to therapeutic cells. In addition, cell embedding offers the outstanding opportunity to insert microenvironment-responsive imaging labels within the hydrogel, paving the way for non-invasive monitoring of the extracellular microenvironment within the hydrogel. We have inserted redox-responsive MRI labels within cell-embedding hydrogels to follow-up the microenvironment redox state.*Methods*High molecular weight chitosan polymers were chemically conjugated with a Gd-HPDO3A-chelate through a disulfide bond, and interspersed within alginate-based hydrogel capsules. Human mesenchymal stem cells (hMSCs) as model therapeutic cells were embedded into such imaging labelled hydrogel. Embedded cells were incubated under simulated hypoxiaconditions, while being followed-up by T1-weighted MRI at 7T.*Results*Under reducing conditions, reductive cleavage of the disulfide bond in the Gd-chitosan probe yields a low molecular weight Gd-chelate that eventually diffuses out of the hydrogel capsule. The resulting change of MRI contrast enhancement along time is very sensitive to the oxygenation level within cell capsules. The kinetics of clearance of contrast enhancement is an indirect indicator of the survival of encapsulated cells.*Conclusions*The Gd-chitosan probe we developed is promising to follow-up non-invasively the redox microenvironment within cellembedding hydrogels. This approach will find useful application to monitor whether transplanted cells succeed to restore normal tissue oxygenation levels, especially in regenerative medicine approaches to ischemic diseases.",
journal = "European Molecular Imaging Meeting - EMIM 2019, March 19-22, 2019, Scottish Event Campus - SEC, Glasgow, UK: Online Program",
title = "Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels"
}
Padovan, S., Catanzaro, V., Capuana, F., Grange, C., Koni, M., Carrera, C., Filipović, N.,& Stevanović, M. (2019). Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels.
European Molecular Imaging Meeting - EMIM 2019, March 19-22, 2019, Scottish Event Campus - SEC, Glasgow, UK: Online Program.
Padovan S, Catanzaro V, Capuana F, Grange C, Koni M, Carrera C, Filipović N, Stevanović M. Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels. European Molecular Imaging Meeting - EMIM 2019, March 19-22, 2019, Scottish Event Campus - SEC, Glasgow, UK: Online Program. 2019;
Padovan Sergio, Catanzaro Valeria, Capuana Federico, Grange Cristina, Koni Malvina, Carrera Carla, Filipović Nenad, Stevanović Magdalena, "Redox-responsive MRI probes to follow-up hypoxia within cell-embedding hydrogels" (2019)

Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats

Dinić, Miroslav; Pecikoza, Uroš; Đokić, Jelena; Stepanović Petrović, Radica; Milenković, Marina; Stevanović, Magdalena; Filipović, Nenad; Begović, Jelena; Golić, Nataša; Lukić, Jovanka

(Lausanne : Frontiers, 2018)

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Pecikoza, Uroš
AU  - Đokić, Jelena
AU  - Stepanović Petrović, Radica
AU  - Milenković, Marina
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Lukić, Jovanka
PY  - 2018
UR  - http://dais.sanu.ac.rs/123456789/2347
AB  - The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1β and iNOS mRNAs in rat’s paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1β, TNF-α and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.
PB  - Lausanne : Frontiers
T2  - Frontiers in Pharmacology
T1  - Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats
SP  - Article 1
VL  - 9
DO  - 10.3389/fphar.2018.00001
ER  - 
@article{
author = "Dinić, Miroslav and Pecikoza, Uroš and Đokić, Jelena and Stepanović Petrović, Radica and Milenković, Marina and Stevanović, Magdalena and Filipović, Nenad and Begović, Jelena and Golić, Nataša and Lukić, Jovanka",
year = "2018",
url = "http://dais.sanu.ac.rs/123456789/2347",
abstract = "The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1β and iNOS mRNAs in rat’s paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1β, TNF-α and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.",
publisher = "Lausanne : Frontiers",
journal = "Frontiers in Pharmacology",
title = "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats",
pages = "Article 1",
volume = "9",
doi = "10.3389/fphar.2018.00001"
}
Dinić, M., Pecikoza, U., Đokić, J., Stepanović Petrović, R., Milenković, M., Stevanović, M., Filipović, N., Begović, J., Golić, N.,& Lukić, J. (2018). Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats.
Frontiers in PharmacologyLausanne : Frontiers., 9, Article 1.
https://doi.org/10.3389/fphar.2018.00001
Dinić M, Pecikoza U, Đokić J, Stepanović Petrović R, Milenković M, Stevanović M, Filipović N, Begović J, Golić N, Lukić J. Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. Frontiers in Pharmacology. 2018;9:Article 1
Dinić Miroslav, Pecikoza Uroš, Đokić Jelena, Stepanović Petrović Radica, Milenković Marina, Stevanović Magdalena, Filipović Nenad, Begović Jelena, Golić Nataša, Lukić Jovanka, "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats" 9 (2018):Article 1,
https://doi.org/10.3389/fphar.2018.00001 .
1
112
13
15

Biodegradable microparticles as scaffolds for cell therapy

Filipović, Nenad; Digilio, Giuseppe; Catanzaro, Valeria; Capuana, Federico; Cutrin, Juan C.; Carniato, Fabio; Porta, Stefano; Grange, Cristina; Stevanović, Magdalena

(Belgrade : Institute of Technical Sciences of SASA, 2018)

TY  - CONF
AU  - Filipović, Nenad
AU  - Digilio, Giuseppe
AU  - Catanzaro, Valeria
AU  - Capuana, Federico
AU  - Cutrin, Juan C.
AU  - Carniato, Fabio
AU  - Porta, Stefano
AU  - Grange, Cristina
AU  - Stevanović, Magdalena
PY  - 2018
UR  - http://dais.sanu.ac.rs/123456789/4721
AB  - Cell therapy is promising strategy that has attracted a lot of attention recently regarding regeneration of diverse tissues and treatment of various pathological conditions. Despite its great potential, several issues still need to be addressed. Among them administration route and dose, microenvironment conditions and host immune response are recognized as a major causes which lead to cells transplantation failure. In this work it is presented novel microstructural system based on biodegradable polymer poly(lactide-co-glycolide) (PLGA) and combination of biocompatible polyvinyl alcohol (PVA) and chitosan, as a scaffold for human mesenchymal stem cells (hMSCs) growth. The obtained microparticles with diameter 200-600 μm showed full biocompatibility with human hMSCs. Besides serving as a solid support, polymeric particles provided controlled release of contrast agent - gadolinium fluoride nanoparticles (Gd-NP) up to 5 weeks. The release of Gd-NP is enhanced by acidic conditions. Magnetic Resonance Imaging (MRI) of the samples embedded in 1% agar showed that contrast enhancement in T1-weighted (T1w) MR images is influenced by the amount of released Gd-NP. Based on these preliminary results, presented theranostic system could be considered for cells grafting.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia
T1  - Biodegradable microparticles as scaffolds for cell therapy
SP  - 7
EP  - 7
ER  - 
@conference{
author = "Filipović, Nenad and Digilio, Giuseppe and Catanzaro, Valeria and Capuana, Federico and Cutrin, Juan C. and Carniato, Fabio and Porta, Stefano and Grange, Cristina and Stevanović, Magdalena",
year = "2018",
url = "http://dais.sanu.ac.rs/123456789/4721",
abstract = "Cell therapy is promising strategy that has attracted a lot of attention recently regarding regeneration of diverse tissues and treatment of various pathological conditions. Despite its great potential, several issues still need to be addressed. Among them administration route and dose, microenvironment conditions and host immune response are recognized as a major causes which lead to cells transplantation failure. In this work it is presented novel microstructural system based on biodegradable polymer poly(lactide-co-glycolide) (PLGA) and combination of biocompatible polyvinyl alcohol (PVA) and chitosan, as a scaffold for human mesenchymal stem cells (hMSCs) growth. The obtained microparticles with diameter 200-600 μm showed full biocompatibility with human hMSCs. Besides serving as a solid support, polymeric particles provided controlled release of contrast agent - gadolinium fluoride nanoparticles (Gd-NP) up to 5 weeks. The release of Gd-NP is enhanced by acidic conditions. Magnetic Resonance Imaging (MRI) of the samples embedded in 1% agar showed that contrast enhancement in T1-weighted (T1w) MR images is influenced by the amount of released Gd-NP. Based on these preliminary results, presented theranostic system could be considered for cells grafting.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia",
title = "Biodegradable microparticles as scaffolds for cell therapy",
pages = "7-7"
}
Filipović, N., Digilio, G., Catanzaro, V., Capuana, F., Cutrin, J. C., Carniato, F., Porta, S., Grange, C.,& Stevanović, M. (2018). Biodegradable microparticles as scaffolds for cell therapy.
Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, SerbiaBelgrade : Institute of Technical Sciences of SASA., 7-7.
Filipović N, Digilio G, Catanzaro V, Capuana F, Cutrin JC, Carniato F, Porta S, Grange C, Stevanović M. Biodegradable microparticles as scaffolds for cell therapy. Program and the Book of Abstracts / Seventeenth Young Researchers' Conference Materials Sciences and Engineering, December 5-7, 2018, Belgrade, Serbia. 2018;:7-7
Filipović Nenad, Digilio Giuseppe, Catanzaro Valeria, Capuana Federico, Cutrin Juan C., Carniato Fabio, Porta Stefano, Grange Cristina, Stevanović Magdalena, "Biodegradable microparticles as scaffolds for cell therapy" (2018):7-7

Polyester micro- and nanospheres for controlled drug delivery, nanomedicine and other biomedical applications: progress and challenges

Stevanović, Magdalena

(2018)

TY  - CONF
AU  - Stevanović, Magdalena
PY  - 2018
UR  - http://dais.sanu.ac.rs/123456789/4668
AB  - Different medicaments (water-soluble vitamins), silver nanoparticles, selenium nanoparticles) have been successfully encapsulated into polyester micro and nanospheres thus creating nanoparticles with the various morphological characteristic. The crucial requirements for the controlled and balanced release of the medicament in the body are their ideal spherical shape and narrow size distribution. The size and shape of particles play the key role in their adhesion and interaction with the cell. Polymer degradation, also, plays a key role in medicament release from sustained release polyester systems, therefore in order to elucidate the mechanism governing release, it appears essential to analyze the in vitro degradation behavior of these devices. An integrated study of the nanosphere composition and structure was carried out by combining different techniques. In vitro degradation process and release tests, cytotoxicity, labeling polyester particles by 99mTc and biodistribution of PLGA nanoparticles without and with encapsulated medicament were examined.
C3  - 8th Annual International Symposium of Drug Delivery Systems – 2018, Theme: Meeting the Challenges with Innovations, Time: July 19-21, 2018 / Place: Saint Petersburg, Russia : Conference Abstract Book of SDDS-2018
T1  - Polyester micro- and nanospheres for controlled drug delivery, nanomedicine and other biomedical applications:  progress and challenges
ER  - 
@conference{
author = "Stevanović, Magdalena",
year = "2018",
url = "http://dais.sanu.ac.rs/123456789/4668",
abstract = "Different medicaments (water-soluble vitamins), silver nanoparticles, selenium nanoparticles) have been successfully encapsulated into polyester micro and nanospheres thus creating nanoparticles with the various morphological characteristic. The crucial requirements for the controlled and balanced release of the medicament in the body are their ideal spherical shape and narrow size distribution. The size and shape of particles play the key role in their adhesion and interaction with the cell. Polymer degradation, also, plays a key role in medicament release from sustained release polyester systems, therefore in order to elucidate the mechanism governing release, it appears essential to analyze the in vitro degradation behavior of these devices. An integrated study of the nanosphere composition and structure was carried out by combining different techniques. In vitro degradation process and release tests, cytotoxicity, labeling polyester particles by 99mTc and biodistribution of PLGA nanoparticles without and with encapsulated medicament were examined.",
journal = "8th Annual International Symposium of Drug Delivery Systems – 2018, Theme: Meeting the Challenges with Innovations, Time: July 19-21, 2018 / Place: Saint Petersburg, Russia : Conference Abstract Book of SDDS-2018",
title = "Polyester micro- and nanospheres for controlled drug delivery, nanomedicine and other biomedical applications:  progress and challenges"
}
Stevanović, M. (2018). Polyester micro- and nanospheres for controlled drug delivery, nanomedicine and other biomedical applications:  progress and challenges.
8th Annual International Symposium of Drug Delivery Systems – 2018, Theme: Meeting the Challenges with Innovations, Time: July 19-21, 2018 / Place: Saint Petersburg, Russia : Conference Abstract Book of SDDS-2018.
Stevanović M. Polyester micro- and nanospheres for controlled drug delivery, nanomedicine and other biomedical applications:  progress and challenges. 8th Annual International Symposium of Drug Delivery Systems – 2018, Theme: Meeting the Challenges with Innovations, Time: July 19-21, 2018 / Place: Saint Petersburg, Russia : Conference Abstract Book of SDDS-2018. 2018;
Stevanović Magdalena, "Polyester micro- and nanospheres for controlled drug delivery, nanomedicine and other biomedical applications:  progress and challenges" (2018)

Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants

Capuana, Federico; Padovan, Sergio; Grange, Cristina; Catanzaro, Valeria; Cutrin, J. C.; Stevanović, Magdalena; Filipović, Nenad; Digilio, G.

(European Society for Molecular Imaging, 2018)

TY  - CONF
AU  - Capuana, Federico
AU  - Padovan, Sergio
AU  - Grange, Cristina
AU  - Catanzaro, Valeria
AU  - Cutrin, J. C.
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Digilio, G.
PY  - 2018
UR  - http://eventclass.org/contxt_emim2018/online-program/session?s=101#153
UR  - http://dais.sanu.ac.rs/123456789/4667
AB  - Introduction: Cell encapsulation by hydrogels is intended to shield transplanted cells from the host hostile environment by preventing the infiltration of host immune cells. Cell scaffolding by solid biocompatible microparticles is intended to provide a structural support to implanted cells and to mimic the extracellular matrix, allowing cells to proliferate and/or differentiate in the desired way. We present strategies to label scaffolding biomaterials with microenvironment responsive MRI probes, for applications in the follow-up of cell transplants.

Methods: Microparticles (MPs) based on PLGA/chitosan were incorporated with gadolinium fluoride nanoparticles (GdNPs), as the MRI T1-contrast agent. The system is designed such to release Gd-NPs in the extracellular matrix (ECM), thus activating MRI contrast, unless MPs are attacked by the immune system (Foreign Body Response, FBR). To proof the concept, PLGA-based MPs were seeded with hMSCs and implanted into either immunocompetent or immunocompromised mice, and the transplants were followed-up by MRI for three weeks. Ex-vivo histologic assessment was carried out at the end of the follow-up.

Results/Discussion: Immunocompetent mice showed poor activation, if any, of MRI contrast within the cell graft. Immunocompromised mice, on the other hand, showed a progressive activation of MRI contrast. Ex-vivo histology showed extensive FBR directed against microparticles in immunocompetent mice, with some surviving hMSCs in the ECM but not on the scaffold surface. No significant FBR was detected in immunocompromised mice, and hMSCs were still adhering to the scaffolds.

Conclusions: The proposed system is able to assess whether or not cell grafts are subjected to innate immune response, an event that is likely correlated to the loss of transplanted cells.
PB  - European Society for Molecular Imaging
C3  - European Molecular Imaging Meeting - EMIM 2018, March 20-23, Kursaal San Sebastian, Spain : Online Program
T1  - Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants
ER  - 
@conference{
author = "Capuana, Federico and Padovan, Sergio and Grange, Cristina and Catanzaro, Valeria and Cutrin, J. C. and Stevanović, Magdalena and Filipović, Nenad and Digilio, G.",
year = "2018",
url = "http://eventclass.org/contxt_emim2018/online-program/session?s=101#153, http://dais.sanu.ac.rs/123456789/4667",
abstract = "Introduction: Cell encapsulation by hydrogels is intended to shield transplanted cells from the host hostile environment by preventing the infiltration of host immune cells. Cell scaffolding by solid biocompatible microparticles is intended to provide a structural support to implanted cells and to mimic the extracellular matrix, allowing cells to proliferate and/or differentiate in the desired way. We present strategies to label scaffolding biomaterials with microenvironment responsive MRI probes, for applications in the follow-up of cell transplants.

Methods: Microparticles (MPs) based on PLGA/chitosan were incorporated with gadolinium fluoride nanoparticles (GdNPs), as the MRI T1-contrast agent. The system is designed such to release Gd-NPs in the extracellular matrix (ECM), thus activating MRI contrast, unless MPs are attacked by the immune system (Foreign Body Response, FBR). To proof the concept, PLGA-based MPs were seeded with hMSCs and implanted into either immunocompetent or immunocompromised mice, and the transplants were followed-up by MRI for three weeks. Ex-vivo histologic assessment was carried out at the end of the follow-up.

Results/Discussion: Immunocompetent mice showed poor activation, if any, of MRI contrast within the cell graft. Immunocompromised mice, on the other hand, showed a progressive activation of MRI contrast. Ex-vivo histology showed extensive FBR directed against microparticles in immunocompetent mice, with some surviving hMSCs in the ECM but not on the scaffold surface. No significant FBR was detected in immunocompromised mice, and hMSCs were still adhering to the scaffolds.

Conclusions: The proposed system is able to assess whether or not cell grafts are subjected to innate immune response, an event that is likely correlated to the loss of transplanted cells.",
publisher = "European Society for Molecular Imaging",
journal = "European Molecular Imaging Meeting - EMIM 2018, March 20-23, Kursaal San Sebastian, Spain : Online Program",
title = "Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants"
}
Capuana, F., Padovan, S., Grange, C., Catanzaro, V., Cutrin, J. C., Stevanović, M., Filipović, N.,& Digilio, G. (2018). Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants.
European Molecular Imaging Meeting - EMIM 2018, March 20-23, Kursaal San Sebastian, Spain : Online ProgramEuropean Society for Molecular Imaging..
Capuana F, Padovan S, Grange C, Catanzaro V, Cutrin JC, Stevanović M, Filipović N, Digilio G. Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants. European Molecular Imaging Meeting - EMIM 2018, March 20-23, Kursaal San Sebastian, Spain : Online Program. 2018;
Capuana Federico, Padovan Sergio, Grange Cristina, Catanzaro Valeria, Cutrin J. C., Stevanović Magdalena, Filipović Nenad, Digilio G., "Biocompatible Materials labelled with Microenvironment Responsive MRI Probes for the follow-up of Cell Transplants" (2018)

Chapter 11 – Polymeric micro- and nanoparticles for controlled and targeted drug delivery

Stevanović, Magdalena

(Elsevier, 2017)

TY  - CHAP
AU  - Stevanović, Magdalena
PY  - 2017
UR  - http://dais.sanu.ac.rs/123456789/2334
AB  - Nanotechnology has great potential in the field of medicine and pharmacy because nano objects have comparable dimensions to biological entities. Polymer-based particles play an integral role as vehicles in the controlled delivery of different forms and types of active substances, such as anticancer drugs, antihypertensive and immunomodulatory agents, medical imaging contrast media, hormones, vitamins, and different macromolecules, such as nucleic acids (deoxyribonucleic acid, ribonucleic acid), proteins, antibodies, etc. The release of the active agent may be constant over a long period, it may be cyclic over a long period, or it may be triggered by the environment or other external events. The purpose behind controlling the drug delivery is to achieve more effective therapies while eliminating the potential for both under and overdosing. Other benefits of using controlled-delivery systems can include the maintenance of drug levels within a desired range, the need for fewer administrations, making optimal use of the drug in question, and increased patient compliance. This review article reports on obtaining polymeric micro- and nanoparticles with a special emphasis on obtaining polyester particles, the incorporation of different active substances within a polymer matrix, the degradation and release process of active substances from the polymeric particles, the physiochemical and biological properties of such obtained systems, as well as their application as drug-delivery systems.
PB  - Elsevier
T2  - Nanostructures for Drug Delivery
T1  - Chapter 11 – Polymeric micro- and nanoparticles for controlled and targeted drug delivery
SP  - 355
EP  - 378
DO  - 10.1016/B978-0-323-46143-6.00011-7
ER  - 
@article{
author = "Stevanović, Magdalena",
year = "2017",
url = "http://dais.sanu.ac.rs/123456789/2334",
abstract = "Nanotechnology has great potential in the field of medicine and pharmacy because nano objects have comparable dimensions to biological entities. Polymer-based particles play an integral role as vehicles in the controlled delivery of different forms and types of active substances, such as anticancer drugs, antihypertensive and immunomodulatory agents, medical imaging contrast media, hormones, vitamins, and different macromolecules, such as nucleic acids (deoxyribonucleic acid, ribonucleic acid), proteins, antibodies, etc. The release of the active agent may be constant over a long period, it may be cyclic over a long period, or it may be triggered by the environment or other external events. The purpose behind controlling the drug delivery is to achieve more effective therapies while eliminating the potential for both under and overdosing. Other benefits of using controlled-delivery systems can include the maintenance of drug levels within a desired range, the need for fewer administrations, making optimal use of the drug in question, and increased patient compliance. This review article reports on obtaining polymeric micro- and nanoparticles with a special emphasis on obtaining polyester particles, the incorporation of different active substances within a polymer matrix, the degradation and release process of active substances from the polymeric particles, the physiochemical and biological properties of such obtained systems, as well as their application as drug-delivery systems.",
publisher = "Elsevier",
journal = "Nanostructures for Drug Delivery",
title = "Chapter 11 – Polymeric micro- and nanoparticles for controlled and targeted drug delivery",
pages = "355-378",
doi = "10.1016/B978-0-323-46143-6.00011-7"
}
Stevanović, M. (2017). Chapter 11 – Polymeric micro- and nanoparticles for controlled and targeted drug delivery.
Nanostructures for Drug DeliveryElsevier., 355-378.
https://doi.org/10.1016/B978-0-323-46143-6.00011-7
Stevanović M. Chapter 11 – Polymeric micro- and nanoparticles for controlled and targeted drug delivery. Nanostructures for Drug Delivery. 2017;:355-378
Stevanović Magdalena, "Chapter 11 – Polymeric micro- and nanoparticles for controlled and targeted drug delivery" (2017):355-378,
https://doi.org/10.1016/B978-0-323-46143-6.00011-7 .
3

PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity

Stanković, Ana; Sezen, Meltem; Milenković, Marina; Kaišarević, Sonja; Andrić, Nebojša; Stevanović, Magdalena

(Hindawi, 2016)

TY  - JOUR
AU  - Stanković, Ana
AU  - Sezen, Meltem
AU  - Milenković, Marina
AU  - Kaišarević, Sonja
AU  - Andrić, Nebojša
AU  - Stevanović, Magdalena
PY  - 2016
UR  - http://dais.sanu.ac.rs/123456789/901
AB  - Copolymer poly (DL-lactide-co-glycolide) (PLGA) is extensively investigated for various biomedical applications such as controlled drug delivery or carriers in the tissue engineering. In addition, zinc oxide (ZnO) is widely used in biomedicine especially for materials like dental composites, as a constituent of creams for the treatment of a variety of skin irritations, to enhance the antibacterial activity of different medicaments and so on. Uniform, spherical ZnO nanoparticles (nano-ZnO) have been synthesized via microwave synthesis method. In addition to obtaining nano-ZnO, a further aim was to examine their immobilization in the PLGA polymer matrix (PLGA/nano-ZnO) and this was done by a simple physicochemical solvent/nonsolvent method. The samples were characterized by X-ray diffraction, scanning electron microscopy, laser diffraction particle size analyzer, differential thermal analysis, and thermal gravimetric analysis. The synthesized PLGA/nano-ZnO particles are spherical, uniform, and with diameters below 1 µm. The influence of the different solvents and the drying methods during the synthesis was investigated too. The biocompatibility of the samples is discussed in terms of in vitro toxicity on human hepatoma HepG2 cells by application of MTT assay and the antimicrobial activity was evaluated by broth microdilution method against different groups of microorganisms (Gram-positive bacteria, Gram-negative bacteria, and yeast Candida albicans).
PB  - Hindawi
T2  - Journal of Nanomaterials
T1  - PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity
SP  - 9425289
VL  - 2016
DO  - 10.1155/2016/9425289
ER  - 
@article{
author = "Stanković, Ana and Sezen, Meltem and Milenković, Marina and Kaišarević, Sonja and Andrić, Nebojša and Stevanović, Magdalena",
year = "2016",
url = "http://dais.sanu.ac.rs/123456789/901",
abstract = "Copolymer poly (DL-lactide-co-glycolide) (PLGA) is extensively investigated for various biomedical applications such as controlled drug delivery or carriers in the tissue engineering. In addition, zinc oxide (ZnO) is widely used in biomedicine especially for materials like dental composites, as a constituent of creams for the treatment of a variety of skin irritations, to enhance the antibacterial activity of different medicaments and so on. Uniform, spherical ZnO nanoparticles (nano-ZnO) have been synthesized via microwave synthesis method. In addition to obtaining nano-ZnO, a further aim was to examine their immobilization in the PLGA polymer matrix (PLGA/nano-ZnO) and this was done by a simple physicochemical solvent/nonsolvent method. The samples were characterized by X-ray diffraction, scanning electron microscopy, laser diffraction particle size analyzer, differential thermal analysis, and thermal gravimetric analysis. The synthesized PLGA/nano-ZnO particles are spherical, uniform, and with diameters below 1 µm. The influence of the different solvents and the drying methods during the synthesis was investigated too. The biocompatibility of the samples is discussed in terms of in vitro toxicity on human hepatoma HepG2 cells by application of MTT assay and the antimicrobial activity was evaluated by broth microdilution method against different groups of microorganisms (Gram-positive bacteria, Gram-negative bacteria, and yeast Candida albicans).",
publisher = "Hindawi",
journal = "Journal of Nanomaterials",
title = "PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity",
pages = "9425289",
volume = "2016",
doi = "10.1155/2016/9425289"
}
Stanković, A., Sezen, M., Milenković, M., Kaišarević, S., Andrić, N.,& Stevanović, M. (2016). PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity.
Journal of NanomaterialsHindawi., 2016, 9425289.
https://doi.org/10.1155/2016/9425289
Stanković A, Sezen M, Milenković M, Kaišarević S, Andrić N, Stevanović M. PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity. Journal of Nanomaterials. 2016;2016:9425289
Stanković Ana, Sezen Meltem, Milenković Marina, Kaišarević Sonja, Andrić Nebojša, Stevanović Magdalena, "PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity" 2016 (2016):9425289,
https://doi.org/10.1155/2016/9425289 .
5
7
8

Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums

Filipović, Nenad; Jeremić, Sanja; Đokić, Lidija; Ražić, Slavica; Stevanović, Magdalena

(Belgrade : Institute of Technical Sciences of SASA, 2016)

TY  - CONF
AU  - Filipović, Nenad
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Ražić, Slavica
AU  - Stevanović, Magdalena
PY  - 2016
UR  - http://dais.sanu.ac.rs/123456789/886
AB  - One of the most prominent properties of poly (є-caprolactone) (PCL) as a biodegradable polymer is slow degradation rate. Due to this advantage the PCL is often used in versatile systems for drug delivery or tissue engineering. When it comes to drug delivery systems, this property of PCL provides the slow release of encapsulated medicaments in order to avoid acute toxicity i.e. to enhance therapeutic efficiency, or protects medicaments from "aggressive" environment and ensures prolonged effect. Selenium nanoparticles (SeNp) recently gained attention as a potential candidate for cancer therapy and prevention with antibacterial properties as well. The major drawback of SeNp is substantial risk of toxicity. Degradation itself is a function of several material properties as well as the nature of surrounding medium. In this work it is examined the release of SeNp from PCL microparticles during the degradation in four different mediums: phosphate buffered saline (PBS), solution of lipase isolated from porcine pancreas in PBS, 0.1 M hydrochloric acid (HCL) and Psseudomonas aeruginosa cell free extract in PBS. The main idea was to compare the release of the selenium nanoparticles in physiological conditions (the first three medium) and in the pathological conditions (the fourth medium), respectively. Firstly, the PCL/SeNp were suspended in adequate medium and placed in water bath at 37 °C. At exact times, samples were collected and examined by different techniques: X-ray diffraction (XRD), inductively coupled plasma-atomic emission spectroscopy (ICP-AES), scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), differential scanning calorimetry (DSC). The release of selenium nanoparticles in physiological conditions occurred in a very slow manner without burst release while in the presence of bacterial extract the release was much more pronounced, even after 24 h.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Fifteenth Young Researchers' Conference Materials Sciences and Engineering, December 7-9, 2016, Belgrade
T1  - Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums
SP  - 8
EP  - 8
ER  - 
@conference{
author = "Filipović, Nenad and Jeremić, Sanja and Đokić, Lidija and Ražić, Slavica and Stevanović, Magdalena",
year = "2016",
url = "http://dais.sanu.ac.rs/123456789/886",
abstract = "One of the most prominent properties of poly (є-caprolactone) (PCL) as a biodegradable polymer is slow degradation rate. Due to this advantage the PCL is often used in versatile systems for drug delivery or tissue engineering. When it comes to drug delivery systems, this property of PCL provides the slow release of encapsulated medicaments in order to avoid acute toxicity i.e. to enhance therapeutic efficiency, or protects medicaments from "aggressive" environment and ensures prolonged effect. Selenium nanoparticles (SeNp) recently gained attention as a potential candidate for cancer therapy and prevention with antibacterial properties as well. The major drawback of SeNp is substantial risk of toxicity. Degradation itself is a function of several material properties as well as the nature of surrounding medium. In this work it is examined the release of SeNp from PCL microparticles during the degradation in four different mediums: phosphate buffered saline (PBS), solution of lipase isolated from porcine pancreas in PBS, 0.1 M hydrochloric acid (HCL) and Psseudomonas aeruginosa cell free extract in PBS. The main idea was to compare the release of the selenium nanoparticles in physiological conditions (the first three medium) and in the pathological conditions (the fourth medium), respectively. Firstly, the PCL/SeNp were suspended in adequate medium and placed in water bath at 37 °C. At exact times, samples were collected and examined by different techniques: X-ray diffraction (XRD), inductively coupled plasma-atomic emission spectroscopy (ICP-AES), scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), differential scanning calorimetry (DSC). The release of selenium nanoparticles in physiological conditions occurred in a very slow manner without burst release while in the presence of bacterial extract the release was much more pronounced, even after 24 h.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Fifteenth Young Researchers' Conference Materials Sciences and Engineering, December 7-9, 2016, Belgrade",
title = "Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums",
pages = "8-8"
}
Filipović, N., Jeremić, S., Đokić, L., Ražić, S.,& Stevanović, M. (2016). Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums.
Program and the Book of Abstracts / Fifteenth Young Researchers' Conference Materials Sciences and Engineering, December 7-9, 2016, BelgradeBelgrade : Institute of Technical Sciences of SASA., 8-8.
Filipović N, Jeremić S, Đokić L, Ražić S, Stevanović M. Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums. Program and the Book of Abstracts / Fifteenth Young Researchers' Conference Materials Sciences and Engineering, December 7-9, 2016, Belgrade. 2016;:8-8
Filipović Nenad, Jeremić Sanja, Đokić Lidija, Ražić Slavica, Stevanović Magdalena, "Comparison of the release of selenium nanoparticles from poly (є-caprolactone) microparticles in four different degradation mediums" (2016):8-8

45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity

Stevanović, Magdalena; Filipović, Nenad; Đurđević, Jelena; Lukić, Miodrag J.; Milenković, Marina; Boccaccini, Aldo

(2015)

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Đurđević, Jelena
AU  - Lukić, Miodrag J.
AU  - Milenković, Marina
AU  - Boccaccini, Aldo
PY  - 2015
UR  - http://dais.sanu.ac.rs/123456789/4670
AB  - n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.
T2  - Colloids and Surfaces B: Biointerfaces
T1  - 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity
SP  - 208
EP  - 215
VL  - 132
DO  - 10.1016/j.colsurfb.2015.05.024
ER  - 
@article{
author = "Stevanović, Magdalena and Filipović, Nenad and Đurđević, Jelena and Lukić, Miodrag J. and Milenković, Marina and Boccaccini, Aldo",
year = "2015",
url = "http://dais.sanu.ac.rs/123456789/4670",
abstract = "n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity",
pages = "208-215",
volume = "132",
doi = "10.1016/j.colsurfb.2015.05.024"
}
Stevanović, M., Filipović, N., Đurđević, J., Lukić, M. J., Milenković, M.,& Boccaccini, A. (2015). 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity.
Colloids and Surfaces B: Biointerfaces, 132, 208-215.
https://doi.org/10.1016/j.colsurfb.2015.05.024
Stevanović M, Filipović N, Đurđević J, Lukić MJ, Milenković M, Boccaccini A. 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity. Colloids and Surfaces B: Biointerfaces. 2015;132:208-215
Stevanović Magdalena, Filipović Nenad, Đurđević Jelena, Lukić Miodrag J., Milenković Marina, Boccaccini Aldo, "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity" 132 (2015):208-215,
https://doi.org/10.1016/j.colsurfb.2015.05.024 .
1
48
47
48

Synthesis of PLGA /nano-ZnO composite particles for biomedical applications

Stanković, Ana; Lukić, Miodrag J.; Jović, Maja; Sezen, Meltem; Milenković, Marina; Stevanović, Magdalena

(Rovinj : International Association of Physical Chemists, 2015)

TY  - CONF
AU  - Stanković, Ana
AU  - Lukić, Miodrag J.
AU  - Jović, Maja
AU  - Sezen, Meltem
AU  - Milenković, Marina
AU  - Stevanović, Magdalena
PY  - 2015
UR  - http://dais.sanu.ac.rs/123456789/857
AB  - Copolymer poly (DL-lactide-co-glycolide) (PLGA), due of its biodegradable and biocompatible nature, is widely used in various medical applications; controlled release of delivering drugs, carriers in the tissue engineering, etc. On the other hand, zinc oxide (ZnO) is extensively used in medicine and pharmacy for personal care products, as well as in biomedical materials like dental composites, as a material for treatment of a variety of skin irritations, to enhance the antibacterial activity of different medicaments, etc. In this research we have dealt with a procedure to prepare particles of poly (lactide-co-glycolide) and nano zinc oxide (PLGA/nano-ZnO). Nano-ZnO has been synthesized using a microwave synthesis method and additionally immobilized within PLGA by physicochemical solvent/non-solvent method. Firstly, ZnO has been dispersed in acetone and then additionally added dropwise in the PLGA/ethyl acetate (PLGA/nano-ZnO(EtAc) or PLGA/acetone (PLGA/nano-ZnO(Ac)) solutions, respectively. The as-prepared dispersions were dried in air atmosphere for 24 h. 
The characterization of the prepared samples was performed using X-ray powder diffraction (XRPD) method for the structure properties, field emission scanning electron microscopy (FE SEM) for the investigation of particles morphology, as well as Malvern’s Mastersizer instrument for particle size distribution. DTA-TG measurements were performed in order to investigate the samples thermal stability and mass loss percentage. The antimicrobial behavior of the synthesized PLGA/nano-ZnO particles was tested against gram-negative and gram-positive bacteria cultures and also against Candida Albicans, diploid fungus.
PB  - Rovinj : International Association of Physical Chemists
C3  - Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK
T1  - Synthesis of PLGA /nano-ZnO composite particles for biomedical applications
ER  - 
@conference{
author = "Stanković, Ana and Lukić, Miodrag J. and Jović, Maja and Sezen, Meltem and Milenković, Marina and Stevanović, Magdalena",
year = "2015",
url = "http://dais.sanu.ac.rs/123456789/857",
abstract = "Copolymer poly (DL-lactide-co-glycolide) (PLGA), due of its biodegradable and biocompatible nature, is widely used in various medical applications; controlled release of delivering drugs, carriers in the tissue engineering, etc. On the other hand, zinc oxide (ZnO) is extensively used in medicine and pharmacy for personal care products, as well as in biomedical materials like dental composites, as a material for treatment of a variety of skin irritations, to enhance the antibacterial activity of different medicaments, etc. In this research we have dealt with a procedure to prepare particles of poly (lactide-co-glycolide) and nano zinc oxide (PLGA/nano-ZnO). Nano-ZnO has been synthesized using a microwave synthesis method and additionally immobilized within PLGA by physicochemical solvent/non-solvent method. Firstly, ZnO has been dispersed in acetone and then additionally added dropwise in the PLGA/ethyl acetate (PLGA/nano-ZnO(EtAc) or PLGA/acetone (PLGA/nano-ZnO(Ac)) solutions, respectively. The as-prepared dispersions were dried in air atmosphere for 24 h. 
The characterization of the prepared samples was performed using X-ray powder diffraction (XRPD) method for the structure properties, field emission scanning electron microscopy (FE SEM) for the investigation of particles morphology, as well as Malvern’s Mastersizer instrument for particle size distribution. DTA-TG measurements were performed in order to investigate the samples thermal stability and mass loss percentage. The antimicrobial behavior of the synthesized PLGA/nano-ZnO particles was tested against gram-negative and gram-positive bacteria cultures and also against Candida Albicans, diploid fungus.",
publisher = "Rovinj : International Association of Physical Chemists",
journal = "Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK",
title = "Synthesis of PLGA /nano-ZnO composite particles for biomedical applications"
}
Stanković, A., Lukić, M. J., Jović, M., Sezen, M., Milenković, M.,& Stevanović, M. (2015). Synthesis of PLGA /nano-ZnO composite particles for biomedical applications.
Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPKRovinj : International Association of Physical Chemists..
Stanković A, Lukić MJ, Jović M, Sezen M, Milenković M, Stevanović M. Synthesis of PLGA /nano-ZnO composite particles for biomedical applications. Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK. 2015;
Stanković Ana, Lukić Miodrag J., Jović Maja, Sezen Meltem, Milenković Marina, Stevanović Magdalena, "Synthesis of PLGA /nano-ZnO composite particles for biomedical applications" (2015)

Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles

Boccaccini, Aldo; Stevanović, Magdalena; Filipović, Nenad; Veselinović, Ljiljana; Lukić, Miodrag J.; Milenković, Marina

(Weimar : Deutsche Gesellschaft für Materialkunde e.V., 2015)

TY  - CONF
AU  - Boccaccini, Aldo
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Lukić, Miodrag J.
AU  - Milenković, Marina
PY  - 2015
UR  - http://dais.sanu.ac.rs/123456789/856
AB  - In the bone tissue engineering field, there is growing interest in the application of bioglass scaffolds due to their bone bonding ability and osteoconductivity. However such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. enhanced bioactivity by incorporation of bioactive molecules or growth factors and effective antibacterial properties. A large number of epidemiological, preclinical, and clinical trials suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. Studies also provide evidence that Se intake may be necessary for bone health. Poly(lactide-co-glycolide) (PLGA) micro and nanoparticles are used for the controlled delivery of several classes of medicaments such as growth factors, antibiotics, antimicrobial agents etc. Uniform, stable, amorphous SeNps have been synthesized and additionally encapsulated within spherical PLGA particles (PLGA/SeNps). Bioglass scaffolds have been synthesized by foam replica method and additionally coated by SeNp or by PLGA with encapsulated SeNp. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, Bioglass®/SeNp and Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections.
PB  - Weimar : Deutsche Gesellschaft für Materialkunde e.V.
C3  - European Symposium and Exhibition on Biomaterials and Related Areas (Euro BioMAT), Weimar, Germany, 2015
T1  - Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles
ER  - 
@conference{
author = "Boccaccini, Aldo and Stevanović, Magdalena and Filipović, Nenad and Veselinović, Ljiljana and Lukić, Miodrag J. and Milenković, Marina",
year = "2015",
url = "http://dais.sanu.ac.rs/123456789/856",
abstract = "In the bone tissue engineering field, there is growing interest in the application of bioglass scaffolds due to their bone bonding ability and osteoconductivity. However such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. enhanced bioactivity by incorporation of bioactive molecules or growth factors and effective antibacterial properties. A large number of epidemiological, preclinical, and clinical trials suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. Studies also provide evidence that Se intake may be necessary for bone health. Poly(lactide-co-glycolide) (PLGA) micro and nanoparticles are used for the controlled delivery of several classes of medicaments such as growth factors, antibiotics, antimicrobial agents etc. Uniform, stable, amorphous SeNps have been synthesized and additionally encapsulated within spherical PLGA particles (PLGA/SeNps). Bioglass scaffolds have been synthesized by foam replica method and additionally coated by SeNp or by PLGA with encapsulated SeNp. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, Bioglass®/SeNp and Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections.",
publisher = "Weimar : Deutsche Gesellschaft für Materialkunde e.V.",
journal = "European Symposium and Exhibition on Biomaterials and Related Areas (Euro BioMAT), Weimar, Germany, 2015",
title = "Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles"
}
Boccaccini, A., Stevanović, M., Filipović, N., Veselinović, L., Lukić, M. J.,& Milenković, M. (2015). Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles.
European Symposium and Exhibition on Biomaterials and Related Areas (Euro BioMAT), Weimar, Germany, 2015Weimar : Deutsche Gesellschaft für Materialkunde e.V...
Boccaccini A, Stevanović M, Filipović N, Veselinović L, Lukić MJ, Milenković M. Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles. European Symposium and Exhibition on Biomaterials and Related Areas (Euro BioMAT), Weimar, Germany, 2015. 2015;
Boccaccini Aldo, Stevanović Magdalena, Filipović Nenad, Veselinović Ljiljana, Lukić Miodrag J., Milenković Marina, "Development and evaluation of 45S5 bioglass scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium nanoparticles" (2015)

45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity

Stevanović, Magdalena; Filipović, Nenad; Đurđević, Jelena; Lukić, Miodrag J.; Milenković, Marina; Boccaccini, Aldo

(2015)

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Đurđević, Jelena
AU  - Lukić, Miodrag J.
AU  - Milenković, Marina
AU  - Boccaccini, Aldo
PY  - 2015
UR  - http://dais.sanu.ac.rs/123456789/758
AB  - n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.
T2  - Colloids and Surfaces B: Biointerfaces
T1  - 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity
SP  - 208
EP  - 215
VL  - 132
DO  - 10.1016/j.colsurfb.2015.05.024
ER  - 
@article{
author = "Stevanović, Magdalena and Filipović, Nenad and Đurđević, Jelena and Lukić, Miodrag J. and Milenković, Marina and Boccaccini, Aldo",
year = "2015",
url = "http://dais.sanu.ac.rs/123456789/758",
abstract = "n the bone tissue engineering field, there is a growing interest in the application of bioactive glass scaffolds (45S5Bioglass®) due to their bone bonding ability, osteoconductivity and osteoinductivity. However, such scaffolds still lack some of the required functionalities to enable the successful formation of new bone, e.g. effective antibacterial properties. A large number of studies suggest that selenium (Se) has significant role in antioxidant protection, enhanced immune surveillance and modulation of cell proliferation. Selenium nanoparticles (SeNp) have also been reported to possess antibacterial as well as antiviral activities. In this investigation, uniform, stable, amorphous SeNp have been synthesized and additionally immobilized within spherical PLGA particles (PLGA/SeNp). These particles were used to coat bioactive glass-based scaffolds synthesized by the foam replica method. Samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). SeNp, 45S5Bioglass®/SeNp and 45S5Bioglass®/PLGA/SeNp showed a considerable antibacterial activity against Gram positive bacteria, Staphylococcus aureus and Staphylococcus epidermidis, one of the main causative agents of orthopedic infections. The functionalized Se-coated bioactive glass scaffolds represent a new family of bioactive, antibacterial scaffolds for bone tissue engineering applications.",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity",
pages = "208-215",
volume = "132",
doi = "10.1016/j.colsurfb.2015.05.024"
}
Stevanović, M., Filipović, N., Đurđević, J., Lukić, M. J., Milenković, M.,& Boccaccini, A. (2015). 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity.
Colloids and Surfaces B: Biointerfaces, 132, 208-215.
https://doi.org/10.1016/j.colsurfb.2015.05.024
Stevanović M, Filipović N, Đurđević J, Lukić MJ, Milenković M, Boccaccini A. 45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity. Colloids and Surfaces B: Biointerfaces. 2015;132:208-215
Stevanović Magdalena, Filipović Nenad, Đurđević Jelena, Lukić Miodrag J., Milenković Marina, Boccaccini Aldo, "45S5Bioglass®-based scaffolds coated with selenium nanoparticles or with poly(lactide-co-glycolide)/selenium particles: Processing, evaluation and antibacterial activity" 132 (2015):208-215,
https://doi.org/10.1016/j.colsurfb.2015.05.024 .
1
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Selenium nanoparticles as a potential candidate in cancer treatment

Filipović, Nenad; Ninić, Jana; Filipič, Metka; Filipović, Miloš; Stevanović, Magdalena

(Rovinj : International Association of Physical Chemists, 2015)

TY  - CONF
AU  - Filipović, Nenad
AU  - Ninić, Jana
AU  - Filipič, Metka
AU  - Filipović, Miloš
AU  - Stevanović, Magdalena
PY  - 2015
UR  - http://dais.sanu.ac.rs/123456789/858
AB  - The broad spectrum of selenium applications in pharmacy and medicine has been known for a while and strongly depends on its chemical form, size and shape. However, the use of Se often requires consumption over the long period, so the toxicity of Se is always a crucial concern. Majority of available pharmaceutical products contain organic forms of selenium or its salts, but recently, when it comes to cancer treatment, elemental selenium nanoparticles (SeNPs) have emerged as a novel selenium source with the advantage of reduced risk of selenium toxicity, but with same bioavailability and efficacy in increasing the activities of selenoenzimes. 
In this work we are presenting the fast, reproducible method for producing stable colloidal suspension of amorphous SeNPs (<80 nm). These SeNPS were successful incorporated within PCL microspheres by combining the high speed homogenization and the precipitation in a solvent/non-solvent system. The obtained PCL/SeNPs were characterized by Fourier transform infrared spectroscopy (FTIR), electron microscopy (SEM and TEM), X-ray diffraction (XRD) and thermal analysis methods (TGA-DTA). The cytotoxicity and the formation of intracellular reactive oxygen species of SeNPs as well as of PCL/SeNPs were investigated employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and using a fluorescent probe (DCFDA test) respectively. Both systems have shown good biocompatibility. The anticancer activity of SeNPs was examined on the HeLa cell line and it was demonstrated that SeNPs exhibits strong, a dose dependent, anticancer activity by preventing further HeLa cells growth and division. Bearing in mind that PCL is well known biodegradable polymer with low degradation rate, it is our opinion that PCL/SeNPs possess a great potential for cancer treatment.
PB  - Rovinj : International Association of Physical Chemists
C3  - Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK
T1  - Selenium nanoparticles as a potential candidate in cancer treatment
ER  - 
@conference{
author = "Filipović, Nenad and Ninić, Jana and Filipič, Metka and Filipović, Miloš and Stevanović, Magdalena",
year = "2015",
url = "http://dais.sanu.ac.rs/123456789/858",
abstract = "The broad spectrum of selenium applications in pharmacy and medicine has been known for a while and strongly depends on its chemical form, size and shape. However, the use of Se often requires consumption over the long period, so the toxicity of Se is always a crucial concern. Majority of available pharmaceutical products contain organic forms of selenium or its salts, but recently, when it comes to cancer treatment, elemental selenium nanoparticles (SeNPs) have emerged as a novel selenium source with the advantage of reduced risk of selenium toxicity, but with same bioavailability and efficacy in increasing the activities of selenoenzimes. 
In this work we are presenting the fast, reproducible method for producing stable colloidal suspension of amorphous SeNPs (<80 nm). These SeNPS were successful incorporated within PCL microspheres by combining the high speed homogenization and the precipitation in a solvent/non-solvent system. The obtained PCL/SeNPs were characterized by Fourier transform infrared spectroscopy (FTIR), electron microscopy (SEM and TEM), X-ray diffraction (XRD) and thermal analysis methods (TGA-DTA). The cytotoxicity and the formation of intracellular reactive oxygen species of SeNPs as well as of PCL/SeNPs were investigated employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and using a fluorescent probe (DCFDA test) respectively. Both systems have shown good biocompatibility. The anticancer activity of SeNPs was examined on the HeLa cell line and it was demonstrated that SeNPs exhibits strong, a dose dependent, anticancer activity by preventing further HeLa cells growth and division. Bearing in mind that PCL is well known biodegradable polymer with low degradation rate, it is our opinion that PCL/SeNPs possess a great potential for cancer treatment.",
publisher = "Rovinj : International Association of Physical Chemists",
journal = "Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK",
title = "Selenium nanoparticles as a potential candidate in cancer treatment"
}
Filipović, N., Ninić, J., Filipič, M., Filipović, M.,& Stevanović, M. (2015). Selenium nanoparticles as a potential candidate in cancer treatment.
Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPKRovinj : International Association of Physical Chemists..
Filipović N, Ninić J, Filipič M, Filipović M, Stevanović M. Selenium nanoparticles as a potential candidate in cancer treatment. Joint Event 4th World Conference on Physico-Chemical Methods in Drug Discovery and Development (PCMDDD-4) and 1st World Conference on ADMET and DMPK. 2015;
Filipović Nenad, Ninić Jana, Filipič Metka, Filipović Miloš, Stevanović Magdalena, "Selenium nanoparticles as a potential candidate in cancer treatment" (2015)

Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity

Stevanović, Magdalena; Bračko, Ines; Milenković, Marina; Filipović, Nenad; Nunić, Jana; Filipič, Metka; Uskoković, Dragan

(Elsevier, 2014)

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Bračko, Ines
AU  - Milenković, Marina
AU  - Filipović, Nenad
AU  - Nunić, Jana
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/574
AB  - A water-soluble antioxidant (ascorbic acid, vitamin C) was encapsulated together with poly(l-glutamic acid)-capped silver nanoparticles (AgNpPGA) within a poly(lactide-co-glycolide) (PLGA) polymeric matrix and their synergistic effects were studied. The PLGA/AgNpPGA/ascorbic acid particles synthesized by a physicochemical method with solvent/non-solvent systems are spherical, have a mean diameter of 775 nm and a narrow size distribution with a polydispersity index of 0.158. The encapsulation efficiency of AgNpPGA/ascorbic acid within PLGA was determined to be >90%. The entire amount of encapsulated ascorbic acid was released in 68 days, and the entire amount of AgNpPGAs was released in 87 days of degradation. The influence of PLGA/AgNpPGA/ascorbic acid on cell viability, generation of reactive oxygen species (ROS) in HepG2 cells, as well as antimicrobial activity against seven different pathogens was investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGA/ascorbic acid particles. We measured the kinetics of ROS formation in HepG2 cells by a DCFH-DA assay, and found that PLGA/AgNpPGA/ascorbic acid caused a significant decrease in DCF fluorescence intensity, which was 2-fold lower than that in control cells after a 5 h exposure. This indicates that the PLGA/AgNpPGA/ascorbic acid microspheres either act as scavengers of intracellular ROS and/or reduce their formation. Also, the results of antimicrobial activity of PLGA/AgNpPGA/ascorbic acid obtained by the broth microdilution method showed superior and extended activity of these particles. The samples were characterized using Fourier transform infrared spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, zeta potential and particle size analysis. This paper presents a new approach to the treatment of infection that at the same time offers a very pronounced antioxidant effect.
PB  - Elsevier
T2  - Acta Biomaterialia
T1  - Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity
SP  - 151
EP  - 162
VL  - 10
IS  - 1
DO  - 10.1016/j.actbio.2013.08.030
ER  - 
@article{
author = "Stevanović, Magdalena and Bračko, Ines and Milenković, Marina and Filipović, Nenad and Nunić, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/574",
abstract = "A water-soluble antioxidant (ascorbic acid, vitamin C) was encapsulated together with poly(l-glutamic acid)-capped silver nanoparticles (AgNpPGA) within a poly(lactide-co-glycolide) (PLGA) polymeric matrix and their synergistic effects were studied. The PLGA/AgNpPGA/ascorbic acid particles synthesized by a physicochemical method with solvent/non-solvent systems are spherical, have a mean diameter of 775 nm and a narrow size distribution with a polydispersity index of 0.158. The encapsulation efficiency of AgNpPGA/ascorbic acid within PLGA was determined to be >90%. The entire amount of encapsulated ascorbic acid was released in 68 days, and the entire amount of AgNpPGAs was released in 87 days of degradation. The influence of PLGA/AgNpPGA/ascorbic acid on cell viability, generation of reactive oxygen species (ROS) in HepG2 cells, as well as antimicrobial activity against seven different pathogens was investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGA/ascorbic acid particles. We measured the kinetics of ROS formation in HepG2 cells by a DCFH-DA assay, and found that PLGA/AgNpPGA/ascorbic acid caused a significant decrease in DCF fluorescence intensity, which was 2-fold lower than that in control cells after a 5 h exposure. This indicates that the PLGA/AgNpPGA/ascorbic acid microspheres either act as scavengers of intracellular ROS and/or reduce their formation. Also, the results of antimicrobial activity of PLGA/AgNpPGA/ascorbic acid obtained by the broth microdilution method showed superior and extended activity of these particles. The samples were characterized using Fourier transform infrared spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, zeta potential and particle size analysis. This paper presents a new approach to the treatment of infection that at the same time offers a very pronounced antioxidant effect.",
publisher = "Elsevier",
journal = "Acta Biomaterialia",
title = "Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity",
pages = "151-162",
volume = "10",
number = "1",
doi = "10.1016/j.actbio.2013.08.030"
}
Stevanović, M., Bračko, I., Milenković, M., Filipović, N., Nunić, J., Filipič, M.,& Uskoković, D. (2014). Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity.
Acta BiomaterialiaElsevier., 10(1), 151-162.
https://doi.org/10.1016/j.actbio.2013.08.030
Stevanović M, Bračko I, Milenković M, Filipović N, Nunić J, Filipič M, Uskoković D. Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity. Acta Biomaterialia. 2014;10(1):151-162
Stevanović Magdalena, Bračko Ines, Milenković Marina, Filipović Nenad, Nunić Jana, Filipič Metka, Uskoković Dragan, "Multifunctional PLGA particles containing poly(l-glutamic acid)-capped silver nanoparticles and ascorbic acid with simultaneous antioxidative and prolonged antimicrobial activity" 10, no. 1 (2014):151-162,
https://doi.org/10.1016/j.actbio.2013.08.030 .
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